| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,489,475 | C→T | 8.9% | intergenic (+32/‑44) | PP_4824 → / → PP_4825 | two‑component system sensor histidine kinase/response regulator/MarC family membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,489,475 | 0 | C | T | 8.9% | 152.4 / 3.2 | 56 | intergenic (+32/‑44) | PP_4824/PP_4825 | two‑component system sensor histidine kinase/response regulator/MarC family membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (33/17); new base T (2/3); total (35/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCAGGCATGGACGGACACATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAGCAAG > NC_002947/5489330‑5489623 | gcaggcaTGGACGGACACATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTcgc > 2:360380/1‑148 (MQ=255) gcaTGGACGGACACATGGCCAAACCCGAGGAGTAGTCGCAGTTGCGTGACCTGATTCAATACTGGCCCAATAACCGGGAAGCCAAGCTGGACCCGGCACAAACGTCCTGACCGATATCCAATCCTCTTGAAGGAGCGGCCACGAGacac > 6:53056/1‑149 (MQ=255) aTGGACGGACACATGGCCAAACCGGTGGAGTTGTCGCAGTTGTGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACaa < 6:363064/149‑1 (MQ=255) gacggacACATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAgg < 5:240871/148‑1 (MQ=255) ggacACATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCcgc > 2:309571/1‑149 (MQ=255) acacATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGCGCGGCCTTGTGTCGCGAGGCcgctc < 8:54064/149‑3 (MQ=255) aTGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGc < 1:360380/149‑1 (MQ=255) tGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCa > 2:117711/1‑149 (MQ=255) ccAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATa > 3:319716/1‑148 (MQ=255) cAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATaaa > 3:300529/1‑149 (MQ=255) cGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAAccc > 1:378996/1‑147 (MQ=255) tGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTTACCGATATCCAATGCTATTGCAGGAGCGGCCTCGCGACACAAGTACGCGCCGGCATAAAACAAAgt > 5:111986/1‑147 (MQ=255) tGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTg > 6:200467/1‑149 (MQ=255) tCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGc < 5:304406/148‑1 (MQ=255) gCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATCCTc > 4:255541/1‑148 (MQ=255) gTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCAt > 2:382582/1‑149 (MQ=255) tGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAgttg > 5:381256/1‑149 (MQ=255) ttCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCCAGGAACACTGCTCATGCTCCATGAgttgtt > 2:297408/1‑148 (MQ=255) ttCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAgttgtt < 6:196958/148‑1 (MQ=255) tCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTTGTGTCGCGAGGCcgc > 8:174256/1‑102 (MQ=255) tCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTTGTGTCGCGAGGCcgc < 7:174256/102‑1 (MQ=255) tGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTa > 6:387710/1‑149 (MQ=255) gggCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTAc > 4:217387/1‑149 (MQ=255) ccAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCt > 8:205570/1‑148 (MQ=255) aaTCACCGGGAAGCCAAGGTGGACCCGGCACTAACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACCAGGCCGCGCCGGCATAAACCCAAGGAACACAGCTGATGCTCCATGAGTAGTTCAGCGTCTACCTGa > 7:18221/1‑148 (MQ=255) cGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCt < 3:217387/149‑1 (MQ=255) cGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCt > 1:60805/1‑149 (MQ=255) gggAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTc < 1:382582/149‑1 (MQ=255) gCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGc > 3:301819/1‑142 (MQ=255) gCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGc < 4:301819/142‑1 (MQ=255) ccAAGGTGGACCCGGCACATAAGTCCTGACCGATATACAATGCTCTTGCAGGAGCGGCCTATCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑Gctc < 5:53056/149‑1 (MQ=255) aaGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTa > 8:41846/1‑149 (MQ=255) gACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGccc > 8:87496/1‑149 (MQ=255) aCCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCg < 5:200467/149‑1 (MQ=255) ggCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑g > 3:212176/1‑133 (MQ=255) ggCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑g < 4:212176/133‑1 (MQ=255) ggCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGttct > 1:263517/1‑149 (MQ=255) cacaTACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGttcttc < 5:387710/149‑1 (MQ=255) tACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGc > 6:220679/1‑149 (MQ=255) aCGTCCTGACCGATATCCAATGATCTTGCAGGAGCGGACTCGCGACACAAGGCCGAGCCGGGATAACCCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCATCGTCTACCTGTAAATGCTCGT‑GCTCTACAGAACGTTCTTCGTGCt > 6:259521/1‑149 (MQ=255) tCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTc > 3:20875/1‑149 (MQ=255) cTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCgt > 7:157742/1‑149 (MQ=255) gACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCgtgt > 8:34072/1‑149 (MQ=255) gACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCgt < 5:308096/147‑1 (MQ=255) gACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCgt > 6:308096/1‑147 (MQ=255) ccAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGc > 3:333578/1‑149 (MQ=255) cAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGcc > 6:66635/1‑149 (MQ=255) ttGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGt > 8:332071/1‑149 (MQ=255) gCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTgg > 5:19658/1‑149 (MQ=255) cAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGc < 7:34072/149‑1 (MQ=255) cAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGc < 2:60805/149‑1 (MQ=255) aGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGcc > 6:314019/1‑149 (MQ=255) cgCGGCCTTGTGTCGCGAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCg > 4:324719/2‑113 (MQ=255) cgCGGCCTTGTGTCGCGAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCg < 3:324719/112‑1 (MQ=255) gCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAg < 1:138334/149‑1 (MQ=255) ccTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGTCGCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAg < 1:309571/149‑1 (MQ=255) ccTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAGc < 5:220679/149‑1 (MQ=255) cgcgACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAGCAAg < 4:333578/149‑1 (MQ=255) | GCAGGCATGGACGGACACATGGCCAAACCGGTGGAGTTGTCGCAGTTGCGTGAGCTGATTCAATACTGGGCCAATCACCGGGAAGCCAAGGTGGACCCGGCACATACGTCCTGACCGATATCCAATGCTCTTGCAGGAGCGGCCTCGCGACACAAGGCCGCGCCGGCATAAACCCAAGGAACACTGCTCATGCTCCATGAGTTGTTCAGCGTCTACCTGAAAATGCTCGT‑GCTCTACAGCCCGTTCTTCGTGCTGTCGTGTTTCATCAGCCTGACCCGTGGCCACTCCAGCAAG > NC_002947/5489330‑5489623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |