Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,061 A→T 9.8% intergenic (+38/+65) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0610AT 9.8% 130.5 / 6.1 57intergenic (+38/+65)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base A (21/26);  new base T (2/3);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

AGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGC  >  NC_002947/5536914‑5537203
                                                                                                                                                    |                                                                                                                                              
aGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAg                                                                                                                                               <  4:100989/149‑1 (MQ=255)
 gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAgg                                                                                                                                              <  4:101565/149‑1 (MQ=255)
 gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAgg                                                                                                                                              <  3:30411/149‑1 (MQ=255)
 gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAgg                                                                                                                                              <  6:299457/149‑1 (MQ=255)
 gATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAgg                                                                                                                                              <  7:281279/149‑1 (MQ=255)
   tCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGt                                                                                                                                            <  5:117026/149‑1 (MQ=255)
   tCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGt                                                                                                                                            <  7:257034/149‑1 (MQ=255)
     gATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTg                                                                                                                                           >  7:84124/1‑148 (MQ=255)
       tGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTGACCCGCGCATACGAGGGTggaa                                                                                                                                        >  3:369982/1‑146 (MQ=255)
       tGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAc                                                                                                                                        <  4:27864/149‑1 (MQ=255)
         ccTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGTGGGTTCACCCTCGTATTCGCGGGTGAAcc                                                                                                                                       <  8:127772/148‑1 (MQ=255)
         ccTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCTCGTATTCGCGGGTGAAcc                                                                                                                                       <  3:78105/148‑1 (MQ=255)
          cTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGAGGGTTCACCCGGGGATAAGAGGGTGAACCCa                                                                                                                                     >  6:390270/1‑149 (MQ=255)
           ttCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAc                                                                                                                                    >  3:18873/1‑149 (MQ=255)
           ttCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAc                                                                                                                                    >  1:313188/1‑149 (MQ=255)
             ccTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAcc                                                                                                                                   <  2:280229/148‑1 (MQ=255)
               tGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGt                                                                                                                                            >  3:32932/1‑137 (MQ=255)
                    gggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCGGAATACGCGGGGGCACCCACCGACGCCtt                                                                                                                           >  8:252032/1‑149 (MQ=255)
                       cAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGACGGTGAACCCACCGACGCATTcg                                                                                                                         >  1:257753/1‑149 (MQ=255)
                       cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                        <  5:153625/149‑1 (MQ=255)
                       cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                        <  4:18873/149‑1 (MQ=255)
                       cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                        <  2:313188/149‑1 (MQ=255)
                         gCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGTAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                      <  3:9038/149‑1 (MQ=25)
                         gCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                      <  5:211390/149‑1 (MQ=25)
                          ccGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                     <  8:84124/149‑1 (MQ=255)
                           cGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAACCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                    <  6:37553/149‑1 (MQ=255)
                             gTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGCATACGAGGGTTAACCCACCCACCCATTAGCGGGTg                                                                                                                   >  3:337611/1‑148 (MQ=255)
                                gACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAAc                                                                                                                <  5:156190/148‑1 (MQ=21)
                                   cAGGCAGTGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                            <  2:7000/149‑1 (MQ=255)
                                                     atcG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCt                                                                                                        <  3:24169/135‑1 (MQ=255)
                                                          tcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCGGGGTTCGCCGTCAGc                                                                                      >  5:347581/1‑149 (MQ=255)
                                                              cACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATATGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTGCGGGCGGGTTCGGCGTCAGCCgtt                                                                                  >  6:83460/1‑149 (MQ=37)
                                                                      gCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCCGAATAAGAGGGTGAACCCCCCGAACCAATACCCGGTGAACCCCCGCCAGCCCGGTTCGCCGGGTGCCGTTGATTaaat                                                                          >  8:187790/1‑146 (MQ=255)
                                                                        acgccccACCCCAAGCGCCACCTCCCGTTCGCTCCTTTATAAGTGCCCTGCTGGAGCGGGTTCACCCGCTAATACGAGGGTCAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                        <  3:237711/145‑1 (MQ=25)
                                                                        aaGCCCCAGCCCAAGCGCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                        <  7:252032/149‑1 (MQ=255)
                                                                        aaGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGATTACGAGGGTGAACCCACCGACGCATTCCCGGGTGCTCCCGCTCCTGCACGGTTCGCCGGCAGCCATTGATTACTTGa                                                                        >  7:258953/1‑149 (MQ=255)
                                                                            cccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCGCCAGCAGGGTTCGCCGTCAGCCTTTGATTACATGACGAt                                                                    >  6:397433/1‑149 (MQ=255)
                                                                             ccaccaCACGCGCCAACTGCCGTACGCTCATTGATATGGGGCCTCCAGGTGCGGGTACACCCGCTAATACTAGGGTTAACCCACCGACGCATGCGGGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATg                                                                   <  7:148356/149‑1 (MQ=255)
                                                                                  acacGCGCGAACTGCCGTTCGCTCCTTGATAAGTGCCGTGCAGCAGCGGGTTCACCCGCGTATACGAGGGGTAACCCACCGACGCATTGGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                              <  5:390270/149‑1 (MQ=255)
                                                                                    aaGCGCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGc                                                            <  5:397433/149‑1 (MQ=255)
                                                                                     aGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCTGCCTTTGATTACTTGACGATGACCTTGcc                                                           <  7:187790/149‑1 (MQ=255)
                                                                                               gcggtgcGCTCCTTTATACGGGGCCGCCCGCCGCGGGTCCCCCCGCTAATACGCGGGCCACCCCACCCACGCATTCGCGGGTCACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                 <  2:206459/143‑1 (MQ=255)
                                                                                                ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                 <  1:131243/148‑1 (MQ=255)
                                                                                                 cGTGCGCTCCTTGATAAGGGGCCTGCCGGAGCGGGTTCCCCCGCTAATACGCGGGTGAACCCACCGACGCATGCGCGGGTGAACCCGCTCCTGCGGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCga                                               <  6:347581/149‑1 (MQ=255)
                                                                                                                                   gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg             >  8:229950/1‑149 (MQ=255)
                                                                                                                                       aCCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac         >  8:355349/1‑149 (MQ=25)
                                                                                                                                        cccGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac         >  6:39464/1‑148 (MQ=25)
                                                                                                                                        cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacc        >  1:269272/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATACGAGGGTGACCCCACCGACGCCTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGCGAAGGTGCAGAAGAAGATGTAGTcacca       >  5:112244/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGAtgt                                          <  6:134124/114‑1 (MQ=255)
                                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGAtgt                                          >  5:134124/1‑114 (MQ=255)
                                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca       >  7:317449/1‑149 (MQ=255)
                                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca       >  2:151459/1‑149 (MQ=255)
                                                                                                                                           gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa              <  2:189692/140‑1 (MQ=255)
                                                                                                                                           gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa              >  1:189692/1‑140 (MQ=255)
                                                                                                                                           gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCaccacc     >  5:120084/1‑149 (MQ=255)
                                                                                                                                              aaTACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGc  <  8:197497/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGC  >  NC_002947/5536914‑5537203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑