Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,079 C→T 10.7% intergenic (+56/+47) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0790CT10.7% 122.9 / 4.8 48intergenic (+56/+47)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base T (2/3);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

GGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCG  >  NC_002947/5536934‑5537222
                                                                                                                                                  |                                                                                                                                               
gggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCGGAATACGCGGGGGCACCCACCGACGCCtt                                                                                                                                              >  8:252032/1‑149 (MQ=255)
   cAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGACGGTGAACCCACCGACGCATTcg                                                                                                                                            >  1:257753/1‑149 (MQ=255)
   cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                                           <  5:153625/149‑1 (MQ=255)
   cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                                           <  2:313188/149‑1 (MQ=255)
   cAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgc                                                                                                                                           <  4:18873/149‑1 (MQ=255)
     gCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGTAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                         <  3:9038/149‑1 (MQ=25)
     gCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                         <  5:211390/149‑1 (MQ=25)
      ccGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCggg                                                                                                                                        <  8:84124/149‑1 (MQ=255)
       cGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAACCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                       <  6:37553/149‑1 (MQ=255)
         gTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGCATACGAGGGTTAACCCACCCACCCATTAGCGGGTg                                                                                                                                      >  3:337611/1‑148 (MQ=255)
            gACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAAc                                                                                                                                   <  5:156190/148‑1 (MQ=21)
               cAGGCAGTGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                                               <  2:7000/149‑1 (MQ=255)
                                 atcG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCt                                                                                                                           <  3:24169/135‑1 (MQ=255)
                                      tcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCGGGGTTCGCCGTCAGc                                                                                                         >  5:347581/1‑149 (MQ=255)
                                          cACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATATGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTGCGGGCGGGTTCGGCGTCAGCCgtt                                                                                                     >  6:83460/1‑149 (MQ=37)
                                                  gCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCCCGAATAAGAGGGTGAACCCCCCGAACCAATACCCGGTGAACCCCCGCCAGCCCGGTTCGCCGGGTGCCGTTGATTaaat                                                                                             >  8:187790/1‑146 (MQ=255)
                                                    acgccccACCCCAAGCGCCACCTCCCGTTCGCTCCTTTATAAGTGCCCTGCTGGAGCGGGTTCACCCGCTAATACGAGGGTCAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                           <  3:237711/145‑1 (MQ=25)
                                                    aaGCCCCAGCCCAAGCGCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                           <  7:252032/149‑1 (MQ=255)
                                                    aaGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGATTACGAGGGTGAACCCACCGACGCATTCCCGGGTGCTCCCGCTCCTGCACGGTTCGCCGGCAGCCATTGATTACTTGa                                                                                           >  7:258953/1‑149 (MQ=255)
                                                        cccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCGCCAGCAGGGTTCGCCGTCAGCCTTTGATTACATGACGAt                                                                                       >  6:397433/1‑149 (MQ=255)
                                                         ccaccaCACGCGCCAACTGCCGTACGCTCATTGATATGGGGCCTCCAGGTGCGGGTACACCCGCTAATACTAGGGTTAACCCACCGACGCATGCGGGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATg                                                                                      <  7:148356/149‑1 (MQ=255)
                                                              acacGCGCGAACTGCCGTTCGCTCCTTGATAAGTGCCGTGCAGCAGCGGGTTCACCCGCGTATACGAGGGGTAACCCACCGACGCATTGGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                                                 <  5:390270/149‑1 (MQ=255)
                                                                aaGCGCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGc                                                                               <  5:397433/149‑1 (MQ=255)
                                                                 aGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCTGCCTTTGATTACTTGACGATGACCTTGcc                                                                              <  7:187790/149‑1 (MQ=255)
                                                                           gcggtgcGCTCCTTTATACGGGGCCGCCCGCCGCGGGTCCCCCCGCTAATACGCGGGCCACCCCACCCACGCATTCGCGGGTCACCCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                                    <  2:206459/143‑1 (MQ=255)
                                                                            ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCatcatc                                                                    <  1:131243/148‑1 (MQ=255)
                                                                             cGTGCGCTCCTTGATAAGGGGCCTGCCGGAGCGGGTTCCCCCGCTAATACGCGGGTGAACCCACCGACGCATGCGCGGGTGAACCCGCTCCTGCGGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCga                                                                  <  6:347581/149‑1 (MQ=255)
                                                                                                               gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg                                >  8:229950/1‑149 (MQ=255)
                                                                                                                   aCCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                            >  8:355349/1‑149 (MQ=25)
                                                                                                                    cccGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                            >  6:39464/1‑148 (MQ=25)
                                                                                                                    cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacc                           >  1:269272/1‑149 (MQ=255)
                                                                                                                     ccGCGAATACGAGGGTGACCCCACCGACGCCTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGCGAAGGTGCAGAAGAAGATGTAGTcacca                          >  5:112244/1‑149 (MQ=255)
                                                                                                                     ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGAtgt                                                             >  5:134124/1‑114 (MQ=255)
                                                                                                                     ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGAtgt                                                             <  6:134124/114‑1 (MQ=255)
                                                                                                                     ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca                          >  7:317449/1‑149 (MQ=255)
                                                                                                                     ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca                          >  2:151459/1‑149 (MQ=255)
                                                                                                                       gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa                                 <  2:189692/140‑1 (MQ=255)
                                                                                                                       gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa                                 >  1:189692/1‑140 (MQ=255)
                                                                                                                       gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCaccacc                        >  5:120084/1‑149 (MQ=255)
                                                                                                                          aaTACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGc                     <  8:197497/149‑1 (MQ=255)
                                                                                                                                 gggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGc              >  6:168440/1‑149 (MQ=255)
                                                                                                                                  ggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCt             >  6:166212/1‑149 (MQ=255)
                                                                                                                                   gTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt            >  8:177024/1‑149 (MQ=255)
                                                                                                                                       aCCCACCGCCGGATTCGCGGGGGAACCCGCTCCTGCTGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGaa        <  8:258953/149‑1 (MQ=255)
                                                                                                                                       aCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                                      >  3:59798/1‑119 (MQ=255)
                                                                                                                                       aCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                                      <  4:59798/119‑1 (MQ=255)
                                                                                                                                           aCCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAgtgt    <  4:337611/149‑1 (MQ=255)
                                                                                                                                             cgtcGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCg  <  2:257753/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCG‑TCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTCG  >  NC_002947/5536934‑5537222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑