Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,566,959 T→A 7.2% Q176L (CAG→CTG)  mutL ← DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,566,9590TA 7.2% 238.9 / 7.3 83Q176L (CAG→CTG) mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base T (41/36);  new base A (3/3);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-02

CATGAAGCCCGGGCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCT  >  NC_002947/5566811‑5567105
                                                                                                                                                    |                                                                                                                                                    
cATGAAGCCCGGGCCGCAGATGGCACCCACCCTCCGTGCAAGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCt                                                                                                                                                      >  5:7075/1‑149 (MQ=255)
  tGAAGCCCGGGCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCTTTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGc                                                                                                                                                    <  3:225524/149‑1 (MQ=255)
    aaGCCCGGGCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGc                                                                                                                                                    >  2:328356/1‑147 (MQ=255)
      gCCCGGGCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTGCAGGAAGt                                                                                                                                                <  6:108527/149‑1 (MQ=255)
          gggCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATc                                                                                                                                           <  5:45831/149‑1 (MQ=255)
            gCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTGCAGGAAGT‑GATccg                                                                                                                                         <  3:84914/149‑3 (MQ=255)
              cGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTGCAGGAAGT‑GATccg                                                                                                                                         <  1:110916/147‑3 (MQ=255)
              cGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTGCAGGAAGT‑GATccg                                                                                                                                         >  2:110916/1‑145 (MQ=255)
              cGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAAt                                                                                                                                       >  7:22694/1‑149 (MQ=255)
              cGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAAt                                                                                                                                       >  7:280892/1‑149 (MQ=255)
              cGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCACGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAAt                                                                                                                                       >  2:238472/1‑149 (MQ=255)
               gCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAAtt                                                                                                                                      >  3:211874/1‑149 (MQ=255)
                      gCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGtttt                                                                                                                              <  6:339451/149‑1 (MQ=255)
                        acccacccGCCGGGCACGGGCGATTTCTGCGTGGGCCTCGGGCAGGCTGAGGATGCGCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGACACGGGCCCGCGCCAGCCGCCGGAGCACTTCCTGCACGT‑GAGCGAATTC‑GGTTGtct                                                                                                                            <  6:132528/149‑1 (MQ=255)
                          ccacccGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGCATCACTTCCTGCAAGT‑GATCGAAGTC‑GGTTTTCTCg                                                                                                                          >  8:305205/1‑149 (MQ=255)
                           cacccGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCgg                                                                                                                         <  1:111549/149‑1 (MQ=255)
                           cacccGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCgg                                                                                                                         >  4:210570/1‑149 (MQ=255)
                              ccgccgTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCtt                                                                                                                      <  6:19916/149‑1 (MQ=255)
                               cgccgTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCttt                                                                                                                     >  7:29368/1‑149 (MQ=255)
                                 ccgTGCACGGGGGATTTCGTCGTGGGCCTCGTGCAGGCTGTGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCTACATCGAAACGGGCCAGCGCCAGCCGCCGGATTATTTCCTGCAAGT‑GATTCAATTC‑GGTTTTCTCGGCTTTGa                                                                                                                   <  7:59622/149‑1 (MQ=255)
                                 ccgTGCACGGGGGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGGCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGa                                                                                                                   <  7:138143/149‑1 (MQ=255)
                                 ccgTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGa                                                                                                                   <  3:210570/149‑1 (MQ=255)
                                         gggCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTg                                                                                                           >  3:320190/1‑149 (MQ=255)
                                         gggCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTg                                                                                                           >  4:13616/1‑149 (MQ=255)
                                            cGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTgcgc                                                                                                        <  1:330412/149‑1 (MQ=255)
                                               tttCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTg                                                                                                     >  6:143456/1‑149 (MQ=255)
                                                   gtcgtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCg                                                                                                 <  4:211874/149‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGTAACTTGCGCCTGGCCggg                                                                                               <  4:205359/148‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCTCCTGGCCgggg                                                                                              <  6:7075/149‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCgggg                                                                                              <  2:247056/149‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCgggg                                                                                              <  3:187153/149‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCggg                                                                                               <  1:328356/148‑1 (MQ=255)
                                                      gtGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCggg                                                                                               <  1:238472/148‑1 (MQ=255)
                                                          gCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTAtt                                                                                          <  7:56851/149‑1 (MQ=255)
                                                            cTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGATATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCGGGCCGGGGTATTg                                                                                         >  4:284253/1‑148 (MQ=255)
                                                            cTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTga                                                                                        <  2:317817/149‑1 (MQ=255)
                                                              cGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTg                                                                                         >  5:329597/1‑146 (MQ=255)
                                                               gTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTgaag                                                                                      >  8:299304/1‑148 (MQ=255)
                                                                   aGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACaa                                                                                 <  8:22694/149‑1 (MQ=255)
                                                                     gCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAAt                                                                               >  4:168187/1‑149 (MQ=255)
                                                                     gCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAAt                                                                               <  7:299304/149‑1 (MQ=255)
                                                                           gATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCa                                                                         >  8:373572/1‑149 (MQ=255)
                                                                           gATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCa                                                                         >  5:271470/1‑149 (MQ=255)
                                                                           gATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCAGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCgcgc                                                                          >  5:276331/1‑148 (MQ=255)
                                                                                tctTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTc                                                                     >  3:328201/1‑148 (MQ=255)
                                                                                tctTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTc                                                                     >  1:291893/1‑148 (MQ=255)
                                                                                               gcAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTaccgacc                                                     >  7:3831/1‑149 (MQ=255)
                                                                                                   gTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGt                                                 >  7:335856/1‑149 (MQ=255)
                                                                                                    tGGAAGCCGCCATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGGAAGG‑GATCGCATTC‑GGGTTTCTCGGCGTTGAGGGACTTGCGGCTGGGCGGGGTATTGAGGAACGAGTCGGGCACTTCCACGGCGGTACCGCCCggggg                                                >  8:216658/1‑147 (MQ=255)
                                                                                                       aaGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTgcgc                                             >  6:151218/1‑149 (MQ=255)
                                                                                                          ccGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGATTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGcgccgc                                          >  4:16644/1‑149 (MQ=255)
                                                                                                           cGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGTGGATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGcgccgc                                          >  1:289585/1‑149 (MQ=255)
                                                                                                           cGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCgccgcc                                         <  1:143874/149‑1 (MQ=255)
                                                                                                           cGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCgccgcc                                         >  1:276311/1‑149 (MQ=255)
                                                                                                                tCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTg                                    >  3:283051/1‑149 (MQ=255)
                                                                                                                  gAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTtc                                                                                                                             <  4:379759/58‑1 (MQ=255)
                                                                                                                  gAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTtc                                                                                                                             >  3:379759/1‑58 (MQ=255)
                                                                                                                   aaaCGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTCGGGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCa                                  >  1:96659/1‑149 (MQ=255)
                                                                                                                    aaCGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCAcc                                >  3:315073/1‑149 (MQ=255)
                                                                                                                    aaCGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCAcc                                >  5:272425/1‑149 (MQ=255)
                                                                                                                      cGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCcg                              >  7:230060/1‑149 (MQ=255)
                                                                                                                         gccagcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCgcgg                           >  7:215128/1‑149 (MQ=255)
                                                                                                                          ccagcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCgcggc                          <  6:271470/149‑1 (MQ=255)
                                                                                                                          ccagcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCgcggc                          <  6:276331/149‑1 (MQ=255)
                                                                                                                          ccagcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCgcggc                          <  4:328201/149‑1 (MQ=255)
                                                                                                                          ccagcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCgcggc                          >  5:255146/1‑149 (MQ=255)
                                                                                                                            agcgccagcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGt                        >  7:243094/1‑149 (MQ=255)
                                                                                                                                  agcCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTc                  <  1:226951/149‑1 (MQ=255)
                                                                                                                                    ccgccgGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTcgc                >  1:273734/1‑149 (MQ=255)
                                                                                                                                     cgccgGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTcgcg               <  8:3831/149‑1 (MQ=255)
                                                                                                                                       ttcgATCACTTGCAGGAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGc             >  1:212185/4‑149 (MQ=255)
                                                                                                                                       ccgGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGc             <  4:315073/149‑1 (MQ=255)
                                                                                                                                          gATCACTTGCAGGAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTAAAGAACAAATCGTGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCtt          >  6:12304/1‑149 (MQ=255)
                                                                                                                                            tCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCg        >  7:38278/1‑149 (MQ=255)
                                                                                                                                            tCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCg        <  4:283051/149‑1 (MQ=255)
                                                                                                                                             cACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCgg       >  6:86044/1‑149 (MQ=255)
                                                                                                                                              aCTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGt      <  2:291893/149‑1 (MQ=255)
                                                                                                                                               cTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGtt     >  8:249797/1‑149 (MQ=255)
                                                                                                                                                  ccTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTcgcg               <  7:214222/136‑1 (MQ=255)
                                                                                                                                                  ccTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTcgcg               >  8:214222/1‑136 (MQ=255)
                                                                                                                                                  ccTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGttt    <  6:255146/147‑1 (MQ=255)
                                                                                                                                                  ccTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGttt    <  3:168187/147‑1 (MQ=255)
                                                                                                                                                  ccTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTc   <  5:143456/148‑1 (MQ=255)
                                                                                                                                                   cTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCt  <  2:96659/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CATGAAGCCCGGGCCGCAGATGGCACCCACCCGCCGTGCACGGGCGATTTCGTCGTGGGCCTCGTGCAGGCTGAGGATGCTCTTGCCATTGTGGCGCAGGTGGAAGCCGACATCGAAACGGGCCAGCGCCAGCCGCCGGATCACTTCCTGCAAGT‑GATCGAATTC‑GGTTTTCTCGGCTTTGAGGAACTTGCGCCTGGCCGGGGTATTGAAGAACAAATCGCGCACTTCCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCT  >  NC_002947/5566811‑5567105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑