| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 96.8% | 101.1 / ‑3.0 | 31 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (18/12); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932494‑1932779 | cggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑ccc < 5:145350/148‑2 (MQ=255) cTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑cccgcgcag > 7:107742/1‑141 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAgg > 7:47213/1‑148 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAGGATATGCGTCCCCTGTATGAGCGGGCTTTC‑CCGCGAAGGGCt > 1:55307/1‑148 (MQ=38) aCATTCCACAGCGGAATTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCACCCCCGCGCCTAAAACGTATATGCGTCCCCTGTCGGAGGGGGC‑TTGCCCGCGAAGGGCTGCAAGGCa < 2:123333/143‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 3:163439/1‑148 (MQ=255) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 6:178022/1‑147 (MQ=255) cgCGGCATTGAAGGTCTAACTAAAATAGCATTAAACGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAATGTATATGCGGCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGt < 6:176960/145‑1 (MQ=25) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAGCCCGCGCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGCCTACGCGGATCa > 2:41554/1‑148 (MQ=38) tctcCCGAAAATAGCTGTAAACGCTGGGGCCGGTTTGCGGCCCGTCGCGGGCACGCCCGCTCCTACAACGGATTTCCGTCCCGTGTCGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:109586/148‑1 (MQ=37) ctcACTAAAATAGCTGTAAAGGTTGGGGCGGATTTGGGGCCCTTGGGGGGCATCCCCGCTCATACAAAGTATATGCTTCCCCTGTAGTAGGGGGG‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg < 2:55307/147‑1 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAAGCGGATCAGc > 4:28713/1‑147 (MQ=255) aGCATTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 6:178776/148‑1 (MQ=255) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 3:58293/148‑1 (MQ=255) gCAGTAAACGCTGGGGCCGCTTGGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATAGGCGGCCCCTGTCGGAGCGGGC‑TGGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCGCTGATCCgcgtagt < 7:17070/148‑7 (MQ=25) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCTACAAAGGCTATGCGTCCCCGGTAGGAGCGGGC‑GTGCCCGCGCAGGGCTGCCAGGCCGCCCCAAATTGTCGCCTCCGCGGCTCCGCGCTCCGGCCGGGGGa > 5:193205/1‑148 (MQ=25) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAACGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt > 8:47580/1‑147 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCAAAAAACGATATTCGTACCCTGTATGAGCCGGC‑TTGCCCGCGAAGGGCAGCAAGGCAGCCCCAAAATGTCGACAACGCGGATCAGCGATCCCGCCCGGTGATGTTCAGCTCCAg > 2:52550/1‑147 (MQ=255) cTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGCGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCCAATTGTCGACTACGCGGCTGAGCGATCCGGCAGGGGGATGTTCAGCTCCAGAATCGAGCAGCTg > 6:98374/1‑148 (MQ=38) gCCCGCTCATACAAAGGATATGCTTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 3:188526/148‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 5:178022/148‑1 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 5:38649/1‑148 (MQ=255) ccGCTCCTACAAAGGATATGCGCCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 7:19352/1‑148 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAt > 6:163091/1‑72 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt < 5:163091/72‑1 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGGTTTCCAGCTCGatat > 7:56853/1‑147 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 1:32869/1‑148 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCAGGCAGGGTGATGTTCAGCTCCAGAATCGACCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 8:152944/1‑148 (MQ=255) gCGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg > 8:9051/1‑148 (MQ=255) cGTCCCCTGTAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 3:99211/1‑148 (MQ=255) tAGGAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt > 1:46955/1‑148 (MQ=255) gAGCGGGC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 4:99211/147‑1 (MQ=255) ggC‑TTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat < 7:110870/148‑1 (MQ=255) | CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932494‑1932779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |