| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 307,866 | +C | intergenic (+881/‑988) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 307,865 | 1 | . | C | 95.7% | 149.0 / ‑0.3 | 46 | intergenic (+880/‑989) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (21/23); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAC > NC_002947/307718‑308007 | aaGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑g > 4:103576/1‑148 (MQ=255) cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGTCAGCCGAAA‑G‑GGta < 2:173717/148‑2 (MQ=255) aTGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgtt > 6:104922/1‑144 (MQ=255) tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTCCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑g > 8:55461/1‑142 (MQ=255) gTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑c < 7:55461/142‑1 (MQ=255) ttCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTAcc > 3:25929/1‑148 (MQ=255) ctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTg > 5:14478/1‑148 (MQ=255) gTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATc < 7:61271/147‑1 (MQ=255) aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTga > 2:175674/1‑148 (MQ=255) ttCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgacga > 4:149169/1‑146 (MQ=255) ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGTGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAg > 6:156300/1‑148 (MQ=255) gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTggg < 1:66014/148‑1 (MQ=255) gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTggg < 1:68908/148‑1 (MQ=255) tgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc < 1:42573/148‑1 (MQ=255) gATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg < 4:104432/148‑1 (MQ=255) gATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg > 3:93305/1‑148 (MQ=255) tGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGTCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc < 2:114682/148‑1 (MQ=255) tGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc < 8:5220/148‑1 (MQ=255) tGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt < 2:48064/148‑1 (MQ=255) cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc < 1:175674/146‑1 (MQ=255) gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAAc < 7:183721/148‑1 (MQ=255) tGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATgg < 2:153783/148‑1 (MQ=255) gcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGaa < 3:103576/147‑1 (MQ=255) ggCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGTCGAAGGCGTTGTCTTCAACATGGAAggc < 1:55332/148‑1 (MQ=255) aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct > 6:49185/1‑148 (MQ=255) aTCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGATGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctgct > 6:143768/1‑148 (MQ=255) gCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCAt < 2:56921/148‑1 (MQ=255) gATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCg > 6:21125/1‑148 (MQ=255) tgtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc > 3:35258/1‑148 (MQ=255) tgtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc < 4:182960/148‑1 (MQ=255) ccTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc < 1:102498/148‑1 (MQ=255) ccTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc < 3:15808/148‑1 (MQ=255) ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga > 4:5560/1‑148 (MQ=255) ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga > 1:69563/1‑148 (MQ=255) cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa > 8:158510/1‑147 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga < 5:49185/145‑1 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc < 1:6469/147‑1 (MQ=255) gCCTGGCCGGCAATTGCAAGACTACCCTTTCGGCTGCCGAAATG‑CGTTACCTGATCGGTGACGTCGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa < 5:156300/148‑1 (MQ=255) cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCGCGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTc > 6:57436/1‑148 (MQ=255) cACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATc < 2:141749/148‑1 (MQ=255) cTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg < 3:5560/148‑1 (MQ=255) cTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg < 8:129782/148‑1 (MQ=255) gCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg > 1:96730/1‑146 (MQ=255) aaGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAg > 4:19562/1‑148 (MQ=255) gACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGcc > 8:14910/1‑148 (MQ=255) gACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGcc > 2:184679/1‑148 (MQ=255) tACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt > 6:17572/1‑147 (MQ=255) cccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa > 6:185328/1‑148 (MQ=255) ccTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa < 2:31167/147‑1 (MQ=255) gAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAc > 2:92848/1‑148 (MQ=255) | AAGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAC > NC_002947/307718‑308007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |