Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7501.G97.9% 156.0 / ‑3.0 47intergenic (+112/+28)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (27/19);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674611‑5674888
                                                                                                                                            |                                                                                                                                           
cccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccccct                                                                                                                                     >  8:27048/1‑141 (MQ=255)
             aCCCGCAAGGGCTGATAGAAGTACCGCGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTAGATCACTACAGGAGCGGCCTTGTGTCGTTATAGGGCCGCCAAGCGG‑CCCCCGCGGTTTTCAGCTg                                                                                                                        >  8:151369/1‑148 (MQ=37)
              cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCt                                                                                                                         <  2:56945/146‑1 (MQ=255)
               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  6:45957/1‑146 (MQ=255)
               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  6:33505/1‑146 (MQ=255)
               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  4:176891/1‑146 (MQ=255)
                cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  7:111323/1‑145 (MQ=255)
                        cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                             <  7:21346/148‑1 (MQ=255)
                         tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                            <  8:42170/148‑1 (MQ=255)
                               aaGTACCTAGGGTGCTGCTTCGCAGACGTTTCACGACACACGGCCGATCCTGCTGAGAATTTGTTGATCTCTACAGGAGCGGCGTTGTGTCGTTATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                       <  6:125688/147‑1 (MQ=255)
                                 gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                    <  1:55657/148‑4 (MQ=25)
                                 gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                    <  7:27048/148‑4 (MQ=25)
                                    ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcaca                                                                                                 >  8:88816/1‑148 (MQ=255)
                                           ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                           >  6:80536/1‑147 (MQ=255)
                                           ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCCCACCTTCg                                                                                           >  8:76468/1‑147 (MQ=37)
                                              tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacag                                                                                       >  7:70308/1‑147 (MQ=255)
                                               gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  8:70308/144‑1 (MQ=255)
                                                 ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCCCACCTTCGACCCTgc                                                                                    >  7:117364/1‑147 (MQ=37)
                                                 ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                     >  5:46721/1‑147 (MQ=255)
                                                      aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                 >  6:178307/1‑146 (MQ=255)
                                                       gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcca                                                                              >  1:165749/1‑148 (MQ=255)
                                                           tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                           >  8:30424/1‑147 (MQ=255)
                                                             tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  4:96230/1‑148 (MQ=255)
                                                             tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  7:82704/1‑148 (MQ=255)
                                                               aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      >  4:124635/1‑148 (MQ=255)
                                                                cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  5:45957/147‑1 (MQ=255)
                                                                 gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                    >  7:184688/1‑148 (MQ=255)
                                                                    acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg                                                                 >  1:59125/1‑148 (MQ=255)
                                                                     caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCGTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                <  5:80536/148‑1 (MQ=255)
                                                                           cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                          <  7:88816/148‑1 (MQ=255)
                                                                                 tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                           >  8:144114/1‑109 (MQ=255)
                                                                                  gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  7:144114/109‑1 (MQ=255)
                                                                                  gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                   >  2:186474/1‑148 (MQ=255)
                                                                                    agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                 >  3:9129/1‑148 (MQ=255)
                                                                                                    tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  7:128349/1‑148 (MQ=255)
                                                                                                    tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  7:151301/1‑148 (MQ=255)
                                                                                                      tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                               >  5:99734/1‑148 (MQ=255)
                                                                                                       aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  6:46721/143‑1 (MQ=255)
                                                                                                         aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                             <  2:176815/147‑1 (MQ=255)
                                                                                                            aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         <  8:111323/148‑1 (MQ=255)
                                                                                                              cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                       <  7:30424/148‑1 (MQ=255)
                                                                                                                  cTTGTGTGGTGATAGGGCCGCAAAGC‑GTCCGCCTCGGTTTTCAGCTGGGAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTATCACCCGGGCCTTTGTTGCCGAAGTCGTTCAGGTTCAGc                   <  3:146639/148‑1 (MQ=255)
                                                                                                                      tgtCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCATGTCCAGGGTCTGCGAAGTAGCAACCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                <  4:171236/147‑1 (MQ=255)
                                                                                                                              tAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc        <  2:165749/147‑1 (MQ=255)
                                                                                                                                 ggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt     >  5:159290/1‑147 (MQ=255)
                                                                                                                                  gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt     >  4:189702/1‑146 (MQ=255)
                                                                                                                                   ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  8:161852/148‑1 (MQ=255)
                                                                                                                                   ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  3:124635/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674611‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑