Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	137.5 / NA	47	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (27/20);  total (27/20)

ACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674624‑5674888                                                                                                                                    |                                                                                                                                        aCCCGCAAGGGCTGATAGAAGTACCGCGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTAGATCACTACAGGAGCGGCCTTGTGTCGTTATAGGGCCGCCAAGCGG‑CCCCCGCGGTTTTCAGCTg                                                                                                                        >  8:151369/1‑148 (MQ=37)  cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCt                                                                                                                         <  2:56945/146‑1 (MQ=255)   ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  6:45957/1‑146 (MQ=255)   ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  6:33505/1‑146 (MQ=255)   ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  4:176891/1‑146 (MQ=255)    cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTg                                                                                                                        >  7:111323/1‑145 (MQ=255)            cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                             <  7:21346/148‑1 (MQ=255)             tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                            <  8:42170/148‑1 (MQ=255)                   aaGTACCTAGGGTGCTGCTTCGCAGACGTTTCACGACACACGGCCGATCCTGCTGAGAATTTGTTGATCTCTACAGGAGCGGCGTTGTGTCGTTATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                       <  6:125688/147‑1 (MQ=255)                     gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                    <  1:55657/148‑4 (MQ=25)                     gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                    <  7:27048/148‑4 (MQ=25)                        ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcaca                                                                                                 >  8:88816/1‑148 (MQ=255)                               ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                           >  6:80536/1‑147 (MQ=255)                               ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCCCACCTTCg                                                                                           >  8:76468/1‑147 (MQ=37)                                  tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacag                                                                                       >  7:70308/1‑147 (MQ=255)                                   gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  8:70308/144‑1 (MQ=255)                                     ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCCCACCTTCGACCCTgc                                                                                    >  7:117364/1‑147 (MQ=37)                                     ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                     >  5:46721/1‑147 (MQ=255)                                          aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                 >  6:178307/1‑146 (MQ=255)                                           gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtcca                                                                              >  1:165749/1‑148 (MQ=255)                                               tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                           >  8:30424/1‑147 (MQ=255)                                                 tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  4:96230/1‑148 (MQ=255)                                                 tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  7:82704/1‑148 (MQ=255)                                                   aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      >  4:124635/1‑148 (MQ=255)                                                    cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  5:45957/147‑1 (MQ=255)                                                     gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                    >  7:184688/1‑148 (MQ=255)                                                        acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTg                                                                 >  1:59125/1‑148 (MQ=255)                                                         caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCGTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                <  5:80536/148‑1 (MQ=255)                                                               cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                          <  7:88816/148‑1 (MQ=255)                                                                     tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCg                                                                                           >  8:144114/1‑109 (MQ=255)                                                                      gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          <  7:144114/109‑1 (MQ=255)                                                                      gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                   >  2:186474/1‑148 (MQ=255)                                                                        agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                 >  3:9129/1‑148 (MQ=255)                                                                                        tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  7:128349/1‑148 (MQ=255)                                                                                        tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGt                                 >  7:151301/1‑148 (MQ=255)                                                                                          tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg                               >  5:99734/1‑148 (MQ=255)                                                                                           aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  6:46721/143‑1 (MQ=255)                                                                                             aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                             <  2:176815/147‑1 (MQ=255)                                                                                                aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         <  8:111323/148‑1 (MQ=255)                                                                                                  cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                       <  7:30424/148‑1 (MQ=255)                                                                                                      cTTGTGTGGTGATAGGGCCGCAAAGC‑GTCCGCCTCGGTTTTCAGCTGGGAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTATCACCCGGGCCTTTGTTGCCGAAGTCGTTCAGGTTCAGc                   <  3:146639/148‑1 (MQ=255)                                                                                                          tgtCGTGATAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCATGTCCAGGGTCTGCGAAGTAGCAACCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                <  4:171236/147‑1 (MQ=255)                                                                                                                  tAGGGCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc        <  2:165749/147‑1 (MQ=255)                                                                                                                     ggCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt     >  5:159290/1‑147 (MQ=255)                                                                                                                      gCCGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt     >  4:189702/1‑146 (MQ=255)                                                                                                                       ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  8:161852/148‑1 (MQ=255)                                                                                                                       ccGCAAAGCGG‑CCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  3:124635/148‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        ACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑G‑CGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674624‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑