Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	88.4 / NA	27	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

TGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCG  >  NC_002947/6013771‑6014020                                                                                                                    |                                                                                                                                        tggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCAACCACAGGGGCAGCACAGGTGGCGAAAGC‑AGTGCCGGACCGTTGGGAGCGGGCGTGCCCGCGGATTGCCGCAACCCCTTTATCTTGTTtccg                                                                                                        >  6:169979/1‑148 (MQ=255)                  tCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                                       <  1:123806/148‑1 (MQ=255)                    gAATAAACGCGACACCTGCTTCGCGGGGCCCCCCCCAAACACACCTGAAGCACAAGTCTAGAACCCATGTGCGGTA‑CAGTGGGACCCGGGGTGGCCGCGAAAGGGCGCCAACCATTTATATTGTTTGCCCCCGCCCCCCccacaaaa                                                                                      >  8:7531/1‑145 (MQ=255)                     actAAACGCCACACCTGCTTCCCGGGCACGCCCGCTCCCACAGCTGCAGCACAGTGCTCGAACGC‑TGTGCCGAACCAGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaacaa                                                                                     <  7:4994/145‑1 (MQ=37)                     aaTAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGCCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaacaa                                                                                     <  7:7531/147‑1 (MQ=255)                          aCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGGGCTGCACTGGTCTCGACCGC‑TTTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTg                                                                               <  5:119467/148‑1 (MQ=38)                           cgcgACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCt                                                                                <  5:169182/146‑1 (MQ=255)                                       ttCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttcttc                                                                   <  3:134291/147‑1 (MQ=255)                                       ttCGCGGGCACGCCCGCTCCCACAGGTCCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                                                                    <  5:192413/146‑1 (MQ=255)                                       ttCGCGGGCACGCCCGCTCCCACAGGGGCAGCACAGGGCGCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                                                                    <  3:157041/146‑1 (MQ=255)                                           cgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTTCCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTaa                                                              >  7:133491/1‑148 (MQ=255)                                           cgGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTa                                                               <  3:70902/147‑1 (MQ=255)                                              gCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGcc                                                           >  6:176541/1‑148 (MQ=255)                                                aCGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCCGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGcccc                                                         >  7:55210/1‑148 (MQ=255)                                                   cccGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                                      <  7:830/148‑1 (MQ=255)                                                       cTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCt                                                  >  4:81475/1‑148 (MQ=255)                                                          ccACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                                               >  4:112039/1‑148 (MQ=255)                                                           cacaGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCACCCCTGTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGGTCTTCTTTAAGCCCCTGCGGCTGccc                                              >  5:3273/1‑148 (MQ=255)                                                           cacaGGTACGGCACAGCGTTCGAGACC‑TGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                                               >  1:132034/1‑147 (MQ=21)                                                               ggTGCAGCACAGGGCTCGAACGC‑TGGCCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAAc                                          <  1:70745/148‑1 (MQ=255)                                                                                    gC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTg                                                 >  5:41936/1‑120 (MQ=255)                                                                                    gC‑TGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCtt                     <  1:167406/148‑1 (MQ=255)                                                                                     c‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                <  6:41936/120‑1 (MQ=255)                                                                                       ggtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc                   <  7:85736/147‑1 (MQ=255)                                                                                           ccGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc               <  6:69873/148‑1 (MQ=255)                                                                                                 cTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTcc         >  8:91846/1‑148 (MQ=255)                                                                                                        aGCGGGCGTGCCCGCGAATTGCCGAAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAAATGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCACCg  <  4:13661/148‑1 (MQ=255)                                                                                                                    |                                                                                                                                        TGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGC‑TGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCG  >  NC_002947/6013771‑6014020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑