Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	38.9 / NA	13	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACA  >  NC_002947/499058‑499345                                                                                                                                                   |                                                                                                                                               aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑ggg                                                                                                                                              >  5:182533/1‑148 (MQ=255)   gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCa                                                                                                                                            >  3:172114/1‑148 (MQ=255)      cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCCTGc                                                                                                                                         >  5:64375/1‑148 (MQ=255)               ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCCCGAAcc                                                                                                                                >  3:150746/1‑147 (MQ=255)                       cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGggcctggc                                                                                                                        <  8:178173/148‑1 (MQ=255)                          gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGCGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATTCCCGCGCACGggccgtgccgg                                                                                                                     >  6:192957/1‑141 (MQ=255)                                  cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGCAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATTCCCGCGCACCGGCCGGGCCGGGGGAtctc                                                                                                             >  7:82616/1‑148 (MQ=255)                                   aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                             >  8:62829/1‑147 (MQ=255)                                                           cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  6:143187/148‑1 (MQ=255)                                                                    tGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGcc                                                                             <  2:161267/146‑1 (MQ=255)                                                                                 ggAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCATGCCC‑CGACCGGGCCTTGCGGGCGGGGGTCGATGGCGGGGCCCGGCCCGTAGCGGGGAAGCCCGCGCCcacctggtt                                                              >  7:81233/1‑142 (MQ=255)                                                                                               tctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑GGGCCTTCCCCCGAACCGGCCTGGCCTGCGGATCTCGATGGCTGAGCCGGCCTCATAGCCGGCCAGCCCCCAACCACAGGGTTCGTGGTTGGGTCg                                                 >  6:38888/1‑147 (MQ=255)                                                                                                                          aCTTGCCGAAGCCTATGTGGGAGC‑GGGCATG‑CCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCtt                      >  7:2998/1‑146 (MQ=255)                                                                                                                                             ggAGC‑GGGCTTGCCCGCTACGGGGCCGGGCCTGCTTATCTCTTTGCCTGAGCGGGCGTCTTCGGGGGATGGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGATCGCCCTacaagg  <  6:137831/148‑3 (MQ=255)                                                                                                                                                   |                                                                                                                                               AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGC‑AGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACA  >  NC_002947/499058‑499345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑