Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7501.G98.1% 169.4 / ‑2.9 52intergenic (+112/+28)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (24/27);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674606‑5674890
                                                                                                                                                 |                                                                                                                                             
gAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggcc                                                                                                                                           >  6:205432/1‑146 (MQ=255)
      ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCg                                                                                                                                     >  1:223143/1‑149 (MQ=255)
        aGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGt                                                                                                                                   <  1:25283/149‑1 (MQ=255)
           gctgctGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccccct                                                                                                                                       >  7:282740/1‑135 (MQ=255)
                tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTc                                                                                                                               <  4:235309/145‑1 (MQ=255)
                tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTc                                                                                                                               >  3:235309/1‑145 (MQ=255)
                  aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCACTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCt                                                                                                                           <  7:30524/147‑1 (MQ=255)
                    ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc                                                                                                                                                >  2:10163/1‑125 (MQ=255)
                    ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc                                                                                                                                                <  1:10163/125‑1 (MQ=255)
                      gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  4:114520/1‑148 (MQ=255)
                      gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  3:24941/1‑148 (MQ=255)
                                    aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAGGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGcca                                                                                                       <  7:160292/149‑3 (MQ=37)
                                    aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       <  7:264714/149‑1 (MQ=255)
                                             gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  5:300545/149‑1 (MQ=255)
                                             gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  5:313008/149‑1 (MQ=255)
                                               gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            <  3:114520/149‑1 (MQ=255)
                                                ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTGTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTAAGCTGGCAGAAATTACTTCTGCTTCAAACCTTCgc                                                                                            >  5:123091/1‑147 (MQ=37)
                                                  ctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                         <  4:24941/149‑1 (MQ=255)
                                                  ctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            <  7:95687/146‑1 (MQ=255)
                                                        cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                   >  5:47248/1‑149 (MQ=255)
                                                        cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                   >  5:127266/1‑149 (MQ=255)
                                                         gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt                                                                                        >  7:100117/1‑143 (MQ=255)
                                                           aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                  >  3:352478/1‑147 (MQ=255)
                                                             cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGAGCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGAC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTATGATTCACACCTTCGACACTGATAtccagg                                                                              >  5:67625/1‑149 (MQ=38)
                                                               cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGCTAtccaggtc                                                                            >  7:259807/1‑149 (MQ=255)
                                                                    aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                       >  5:179421/1‑149 (MQ=255)
                                                                         acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                      >  5:114061/1‑110 (MQ=25)
                                                                         acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                      <  6:114061/113‑4 (MQ=25)
                                                                           aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                 >  3:164126/1‑148 (MQ=255)
                                                                            aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                               <  5:205432/149‑1 (MQ=255)
                                                                              gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAg                                                              >  5:291824/1‑148 (MQ=255)
                                                                              gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt                                                             <  4:352478/149‑1 (MQ=255)
                                                                                       gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                    >  1:193365/1‑149 (MQ=255)
                                                                                        cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                   >  2:296206/1‑149 (MQ=255)
                                                                                            gaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                               <  2:223143/149‑1 (MQ=255)
                                                                                              aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                              <  6:179421/148‑1 (MQ=255)
                                                                                                  cTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATg                                          <  1:133745/148‑1 (MQ=255)
                                                                                                     gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                       <  6:47248/148‑1 (MQ=255)
                                                                                                          ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                       <  2:88145/95‑1 (MQ=255)
                                                                                                          ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                       >  1:88145/1‑95 (MQ=255)
                                                                                                           tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                          >  5:65201/1‑107 (MQ=255)
                                                                                                           tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                          <  6:65201/107‑1 (MQ=255)
                                                                                                           tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    <  6:67625/145‑1 (MQ=255)
                                                                                                           tACAGCAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCACCTGGCAGAAAATACTTATGCTTCACACCTTCGACACAGATATCAACATCAAGGGTCTGACAACTAGCACCCTGGCCATTTAAGCCGAAGACgt                                >  4:15959/1‑149 (MQ=19)
                                                                                                            aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcc                                                                           <  2:67281/105‑1 (MQ=255)
                                                                                                            aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcc                                                                           >  1:67281/1‑105 (MQ=255)
                                                                                                             cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttc                              <  7:293765/149‑1 (MQ=255)
                                                                                                               ggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcag                            <  6:291824/149‑1 (MQ=255)
                                                                                                                gAGCGGCCTTGTGTCGTTATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACTCTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                                <  7:260520/128‑1 (MQ=255)
                                                                                                                     gCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttcag                      <  7:75546/149‑1 (MQ=255)
                                                                                                                          gtgtCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg                 >  6:317509/1‑149 (MQ=255)
                                                                                                                                  aTAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc          <  2:193365/148‑1 (MQ=255)
                                                                                                                                     gggCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt       >  5:27927/1‑148 (MQ=255)
                                                                                                                                       tcctcAAAGC‑GGGCCCCTCGGTTTTCAGCTTGCAGAAATTACTTCTTCTTGACTCCTTCGACAATGATATCCAGGTCCAGGGTCTGCTAAGTAGCACCCGGGCCTTTGATGCCGATGTCGTTGAGGTTCAGCGTGGTGTTGGCGTTGaa    <  3:15959/145‑1 (MQ=255)
                                                                                                                                       gCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  6:27927/149‑1 (MQ=255)
                                                                                                                                           cAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  5:156651/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674606‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑