Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	96.1%	164.1 / NA	51	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/23);  minor base T (0/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921315‑2921605                                                                                                                                                  |                                                                                                                                                  cacGTGGCTGCGGGCGGCCGGTAACGACAACCTGACCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCaggg                                                                                                                                                <  1:159516/149‑1 (MQ=255)   cGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCaggggg                                                                                                                                              <  6:230514/149‑1 (MQ=255)    gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggggg                                                                                                                                             >  1:44495/1‑149 (MQ=255)       gCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                           <  6:95518/148‑1 (MQ=255)             ggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACg                                                                                                                                    <  2:140882/149‑1 (MQ=255)                   cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTcc                                                                                                                              >  3:266896/1‑149 (MQ=255)                     gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                             >  4:34535/1‑148 (MQ=255)                        aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCaa                                                                                                                          <  2:44495/148‑1 (MQ=255)                          gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                       >  2:98997/1‑149 (MQ=255)                            cAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTg                                                                                                                     <  3:34535/149‑1 (MQ=255)                            cAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTg                                                                                                                     <  1:98997/149‑1 (MQ=255)                             aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGt                                                                                                                      <  2:138510/147‑1 (MQ=255)                             aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGt                                                                                                                      >  1:138510/1‑147 (MQ=255)                               ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                  >  5:168514/1‑149 (MQ=255)                                        gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                          >  3:198703/1‑148 (MQ=255)                                         ggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa                                                                                                        >  4:354751/1‑149 (MQ=255)                                                    tGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGtggtgg                                                                                             >  2:373369/1‑149 (MQ=255)                                                      aCAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggt                                                                                           <  5:374286/149‑1 (MQ=255)                                                        aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                    <  6:371136/122‑1 (MQ=255)                                                        aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                    >  5:371136/1‑122 (MQ=255)                                                         gCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGtgggtggg                                                                                        <  5:75025/149‑1 (MQ=255)                                                            ccgagctcggcaacggcaaTGACACCCTGCAGGGCGGAGTTGGTAACTACACATTGTTCGGCGAGGTGGGTACCGACACGCTGATCTGGGGGGACGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGc                                                                                      <  2:40382/141‑1 (MQ=255)                                                                  gcggcCACGGCAATGACACCCTGCAGGCCGGCGATGGTAACGACATATTGTTCGGCGAGGTGGGTACCGACAACCTGATGTGGGTGGCCGGCAACGACTTCCTCTACGGTGCGGCATTTAACGATACGGTGGTGGGTGTGGCCGGTAAc                                                                               <  2:161722/149‑1 (MQ=255)                                                                  gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAc                                                                               >  2:308298/1‑149 (MQ=255)                                                                    ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGCCACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCCACGGTGCGGCAGGTAACGATACGGTGGTGGGTGCGGcccgatcgg                                                                              >  1:40382/1‑141 (MQ=255)                                                                    ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                                  <  6:122517/96‑1 (MQ=255)                                                                    ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                                  >  5:122517/1‑96 (MQ=255)                                                                      caacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGaca                                                                           <  3:354751/149‑1 (MQ=255)                                                                          ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                       >  6:54084/1‑149 (MQ=255)                                                                              aTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                                   <  1:373369/149‑1 (MQ=255)                                                                                 acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGt                                                                                  <  1:368664/131‑1 (MQ=255)                                                                                 acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGt                                                                                  >  2:368664/1‑131 (MQ=255)                                                                                 acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGc                                                                 >  3:153779/1‑148 (MQ=255)                                                                                    cccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAtagatag                                                              >  7:46042/1‑142 (MQ=255)                                                                                     ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGAAACCATGATCGCCAcc                                                             >  3:239621/1‑148 (MQ=255)                                                                                          aGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATgg                                                        >  6:74837/1‑148 (MQ=255)                                                                                            ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCa                                                                        >  5:83881/1‑130 (MQ=255)                                                                                            ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCa                                                                        <  6:83881/130‑1 (MQ=255)                                                                                                     ggttaagACACATTGGTGGGCGACGTGTGTTACGACACCCTGTTCATGGGGGGCGGGAAAGTATTTCTTAACGGTGCGGCATGTAAAGATAAGGTGGTGGGTGGGGCCGGTAACGTCACCATGTTCGGCACCGTTGGTAACGATgtgt                                             <  4:198703/142‑1 (MQ=255)                                                                                                      gTAAGGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           <  7:194109/149‑1 (MQ=255)                                                                                                      gTAACGACACATTGTTCGGCGACGTTGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGtt                                            <  5:74837/148‑1 (MQ=255)                                                                                                               caTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCt                                  <  4:266896/149‑1 (MQ=255)                                                                                                                aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                                 >  5:269452/1‑149 (MQ=255)                                                                                                                  tGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                                 >  7:257493/1‑147 (MQ=255)                                                                                                                    ttCGGCGACGTGGGTACCGACACCCTGATCAGGGTGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                              <  1:308298/148‑1 (MQ=255)                                                                                                                        gCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAAGGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt                         >  1:284660/1‑149 (MQ=255)                                                                                                                        gCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt                         <  6:269452/149‑1 (MQ=255)                                                                                                                         cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                        <  6:168514/149‑1 (MQ=255)                                                                                                                               gggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc                   >  6:239343/1‑148 (MQ=255)                                                                                                                                     cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCTTGATCGCCACCGATGGAAACGATGTGTTc                                           <  1:26990/118‑1 (MQ=255)                                                                                                                                     cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                           >  2:26990/1‑118 (MQ=255)                                                                                                                                           ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTTGTGTGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc       >  4:264263/1‑148 (MQ=255)                                                                                                                                               aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  <  3:264263/149‑1 (MQ=255)                                                                                                                                               aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  >  1:157175/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921315‑2921605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑