Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	142.6 / NA	42	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499060‑499344                                                                                                                                                |                                                                                                                                              gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                                          >  5:124014/1‑149 (MQ=255)    cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGcc                                                                                                                                       >  6:261894/1‑149 (MQ=255)    cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGca                                                                                                                                       >  7:2461/1‑148 (MQ=255)         gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                                   >  2:83856/1‑148 (MQ=255)         gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                                   >  2:279740/1‑148 (MQ=255)          cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGaa                                                                                                                                 <  1:184110/149‑1 (MQ=255)             ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                              >  3:202611/1‑149 (MQ=255)                  aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                                         >  7:226121/1‑149 (MQ=255)                  aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                                         <  7:46129/149‑1 (MQ=255)                  aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg                                                                                                                         <  7:239338/149‑1 (MQ=255)                      aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggcct                                                                                                                     >  1:317039/1‑149 (MQ=255)                       cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctggcctg                                                                                                                    <  6:131281/149‑1 (MQ=255)                        gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                                                   <  4:202611/149‑1 (MQ=255)                             aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                               <  1:279740/148‑1 (MQ=255)                                ccgAAGTTGAACTGTAACGCGCTGGCGGAGCGGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggc                                                                                                                            <  1:256542/130‑1 (MQ=255)                                cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                              <  5:7591/130‑1 (MQ=255)                                cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                              >  6:7591/1‑130 (MQ=255)                                cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGAGCTGATTGCGACATCCGGGTGCCCGAAACTTGCCGAAGCCTAGGTGGGCGCGGGCATGCCCGCGACCGggc                                                                                                                            >  2:256542/1‑132 (MQ=255)                                 aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCAGAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGTCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTa                                                                                                          <  1:91128/149‑1 (MQ=255)                                   aaGTTGAAAGTGAACTCGCTGGCGGAGCTCGTCAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGAGCCCGAAACTTGCCGAAGCCTATGTGGGAGCggg                                                                                                                                             >  1:118976/1‑112 (MQ=255)                                        gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTAGGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                                                   <  7:203418/133‑1 (MQ=255)                                             tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATTTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                              <  5:124893/149‑1 (MQ=255)                                                    gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCTGCCCGCGACCGGGCCTGGGCTGCGGATCTCTATGGCTGAGCCGGGCGCTTc                                                                                       >  4:322583/1‑149 (MQ=255)                                                        gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                   <  2:317039/149‑1 (MQ=255)                                                                 gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                                                   <  2:221542/108‑1 (MQ=255)                                                                 gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgc                                                                                                                   >  1:221542/1‑108 (MQ=255)                                                                     aGTACGCCATGGACCACACGCTTATCTGATGGCGACATACGGGGCCCCCAAACTTCCCGACGCCAATGTGGGCGCGGCCTGGCCCGCTAACGGGCCTGGCCTGCGGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTc                                                                       <  4:226325/148‑1 (MQ=255)                                                                      gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                             <  1:318429/125‑1 (MQ=255)                                                                      gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                             >  2:318429/1‑125 (MQ=255)                                                                       tACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCAAGGGGCTGGGCGGCGGTTATGAATTGCTGCGGCGGCCGCGTAGCGGGCCTGGCCCCGcccc                                                                    >  3:169928/1‑148 (MQ=255)                                                                         cGCCAGGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTTTGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCcaca                                                                  <  7:85690/149‑1 (MQ=39)                                                                                   cacaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTgg                                                        >  5:282060/1‑149 (MQ=255)                                                                                    acaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCCCAGGTTTCGTga                                                        >  7:88828/1‑147 (MQ=255)                                                                                               tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                                             <  6:282060/148‑1 (MQ=255)                                                                                                gatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAgg                                           >  3:306027/1‑149 (MQ=255)                                                                                                 atTGCGACATCCGGGTGCCCGAAACTTGCCGACGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTCTGGCTTAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAg                                            <  5:261894/147‑1 (MQ=39)                                                                                                             ggggGCCCGAAACTTGCCGACGCCTATGTGGTAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTTTGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc                              <  1:83856/149‑1 (MQ=39)                                                                                                              ggTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCGCACCGGCCAGGCCGGGGGATATCTATGGCTGAGCCGGCCTCATAGCGGGCAAGCCCGCACCCACAGGTTTCGTGGTTGGCTCCCAAGGGCATTcccaaaccc                             >  2:307343/1‑142 (MQ=255)                                                                                                                     gAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCTGATCTCTATGGGTGAGCCGGCCTCTTCGCGGGCCTGCCCGCTCCCACAGGTTTGGTGGTTGGCGCGCAAGGGCCTTCCAATCCCGCCATccg                      >  2:223179/1‑148 (MQ=255)                                                                                                                      aaaCTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCtt                     <  1:223179/149‑1 (MQ=255)                                                                                                                      aaaCTTCCCTACGCCTATGGGGGCGCGGGCATGCCCGCTAACGGGCCGGGCCGGCTTAGCTCTATGGCTGAGCCGGCCTCTTCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCtt                     <  3:322583/149‑1 (MQ=37)                                                                                                                                     tATGTGGGAGCGGGCATGCCCGCCAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAgg                                           <  5:316632/112‑1 (MQ=255)                                                                                                                                          gggAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGATTCGTGGTTGGCTCCCAAGGGCATTCCAATCCCGCCATCCATCTAGGGCGATCCATacaag  >  5:276615/1‑147 (MQ=38)                                                                                                                                                |                                                                                                                                              GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499060‑499344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑