| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 87.5% | 40.3 / ‑1.2 | 27 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (5/16); minor base A (3/0); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.3% | 66.7 / ‑4.0 | 27 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (9/17); minor base C (0/1); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TC‑GGG‑‑CA‑‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499378‑1499642 || ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGAt > 2:110908/1‑149 (MQ=25) aTTCAGAGCCTCAACGAACCCTTGCCACCACAGCAGGCCTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGcc < 5:232901/149‑1 (MQ=18) tCAGAGCCTGAACCAAGCCTTGCCACCACAGCCGGACTGCGGCGGCCAACCGGGGGGGGGGGGCGTGCCCGCTAAACAGGCGCCGGGGTGCAGGGCACGGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCa < 6:131971/148‑1 (MQ=16) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 7:195855/142‑1 (MQ=21) cgCCTTGCCACCACAGCAGGACTGAGGCTCCCAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTGC‑GC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAg < 1:334360/137‑1 (MQ=18) aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 4:305657/149‑1 (MQ=21) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TAGC‑GGG‑‑CA‑‑CGCCCGCGCCCCCAGACTGATTTCCATTAAGCGGGCCGATAGCTACAGt > 3:265072/1‑149 (MQ=21) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGCTTCCAGCGAAAACCGCGCCGCGGTGCATTGCACCCGCGTTGCCCGTGT‑TGGCGGGG‑‑CC‑‑CC‑CCGCGACCACACACTGATTGCCATTAACCGGGCCAATCGCGTCAGAGACgg > 6:272630/1‑149 (MQ=255) acggcggCTGCCAACGGGTGGGAGCGGGCGGGCCCGCTAAACTGGCGCGGGGGTGCAGGGCACCGGCTTGGCGGGGGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 4:142990/144‑1 (MQ=19) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGTGGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 7:294001/149‑1 (MQ=21) atgCTGCGAATCGGTGGGTGCGGGCGTGCCCGCGAATCAGGCGCCGCGGTGCAGGGCTCCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑GGCCCTCTCCCACAGTCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 5:272630/145‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGGCTCA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 1:110908/148‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt > 1:225631/1‑149 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 3:169986/149‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg > 1:228572/1‑149 (MQ=21) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TAGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt > 1:42049/1‑149 (MQ=25) ccAAACGGTGGGCGCGGGCGTGCCCGCTAAACAGGGGCCGCGGTGCATGGCACGGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt < 4:64777/148‑1 (MQ=25) ccAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc < 4:140810/149‑1 (MQ=21) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc > 2:162143/1‑149 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 3:253382/1‑149 (MQ=37) cccGCTAAACTGGCGCCGCGGGGCAGGCCCCCGGCTTTGCCGGTGT‑T‑CG‑CGG‑‑CAACCC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 5:241240/149‑1 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 6:135857/1‑148 (MQ=37) cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 2:228572/149‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg < 2:225631/149‑1 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:326800/149‑1 (MQ=37) gccgcGTTGCATGGCACCGGCTTTGCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 3:60397/149‑1 (MQ=37) gCCGGTGT‑TCGC‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtc < 5:135857/148‑1 (MQ=255) ttcgc‑GGG‑‑CA‑‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa > 7:365014/5‑149 (MQ=255) || GGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TC‑GGG‑‑CA‑‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499378‑1499642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |