Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,221,923 Δ1 bp 6.9% coding (667/840 nt) PP_4602 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,221,9230G. 6.9% 184.5 / 7.0 58coding (667/840 nt)PP_4602AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base . (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

CTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGCTCGTCCACT  >  NC_002947/5221783‑5222059
                                                                                                                                            |                                                                                                                                        
cTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGatcatc                                                                                                                                   <  4:257660/148‑1 (MQ=255)
   gcgcGGCGTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGcc                                                                                                                               <  3:102513/149‑1 (MQ=255)
        gcgTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGc                                                                                                                          <  1:117626/149‑1 (MQ=255)
        gcgTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGc                                                                                                                          >  7:233949/1‑149 (MQ=255)
          gTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGt                                                                                                                        <  2:70643/149‑1 (MQ=255)
               gCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGt                                                                                                                   <  1:72664/149‑1 (MQ=255)
                   gTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGaa                                                                                                               >  8:173355/1‑149 (MQ=255)
                   gTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGaa                                                                                                               >  5:113454/1‑149 (MQ=255)
                           ctTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCt                                                                                                       >  3:83219/1‑149 (MQ=255)
                            ttGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCtt                                                                                                      >  3:60297/1‑149 (MQ=255)
                                    gcggcTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATa                                                                                                <  6:113454/147‑1 (MQ=255)
                                    gcggcTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAgc                                                                                              <  5:588/149‑1 (MQ=255)
                                           aaGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAg                                                                                       >  6:180417/1‑149 (MQ=255)
                                            aGGCNCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGc                                                                                      >  5:62517/1‑149 (MQ=255)
                                                gcTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTg                                                                                                     >  6:57529/1‑130 (MQ=255)
                                                gcTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTg                                                                                                     <  5:57529/130‑1 (MQ=255)
                                                gcTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTggg                                                                                  <  7:9281/149‑1 (MQ=255)
                                                    tgGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGcc                                                                                <  4:110553/147‑1 (MQ=255)
                                                      gTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTgg                                                                                                                 <  5:58619/112‑1 (MQ=255)
                                                      gTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTgg                                                                                                                 >  6:58619/1‑112 (MQ=255)
                                                       tCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACgg                                                                            >  6:109262/1‑148 (MQ=255)
                                                             tAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATAc                                                                      <  8:233949/148‑1 (MQ=255)
                                                             tAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATAc                                                                      <  1:258278/148‑1 (MQ=255)
                                                                ccGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCgg                                                                   >  3:54085/1‑148 (MQ=255)
                                                                  gCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGAGCTCCAGCTGGGCCACGGAAATACCGGCaa                                                                >  6:264770/1‑149 (MQ=255)
                                                                    aGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATg                                                              <  6:62517/149‑1 (MQ=255)
                                                                     gcgcATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCGCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGc                                                             <  4:217533/149‑1 (MQ=255)
                                                                             ccACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTg                                                                                                     >  4:20237/1‑101 (MQ=255)
                                                                             ccACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTg                                                                                                     <  3:20237/101‑1 (MQ=255)
                                                                              cACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCgg                                                           >  5:140975/1‑142 (MQ=255)
                                                                              cACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCgg                                                           <  6:140975/142‑1 (MQ=255)
                                                                                        aTCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGAGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGc                                           <  5:264770/148‑1 (MQ=255)
                                                                                            gCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATc                                      >  1:52244/1‑149 (MQ=255)
                                                                                            gCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATc                                      >  1:85876/1‑149 (MQ=255)
                                                                                            gCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATc                                      <  2:85876/149‑1 (MQ=255)
                                                                                            gCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGT‑GATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCg                                     >  8:18354/1‑149 (MQ=255)
                                                                                            gCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGT‑GATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCg                                     >  8:22349/1‑149 (MQ=255)
                                                                                               gCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCGTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGc                                   <  7:191388/149‑1 (MQ=255)
                                                                                                 tCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTg                                 >  7:85622/1‑149 (MQ=255)
                                                                                                   cGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATa                                                                                                >  4:162727/1‑84 (MQ=255)
                                                                                                   cGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATa                                                                                                <  3:162727/84‑1 (MQ=255)
                                                                                                   cGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGT‑GATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGt                               <  7:18354/148‑1 (MQ=255)
                                                                                                   cGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGT‑GATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGt                               <  7:22349/148‑1 (MQ=255)
                                                                                                      aTGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGa                             >  3:173546/1‑148 (MQ=255)
                                                                                                       tGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGaaa                           <  4:54085/149‑1 (MQ=255)
                                                                                                            cagGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCt                      >  3:72719/1‑149 (MQ=255)
                                                                                                             agGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTg                     <  2:52244/149‑1 (MQ=255)
                                                                                                                   tGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCATTAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAAt               <  7:173355/149‑1 (MQ=255)
                                                                                                                   tGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAAt               <  2:96695/149‑1 (MQ=255)
                                                                                                                       cATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGc           >  6:43432/1‑149 (MQ=255)
                                                                                                                       cATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGc           >  2:218437/1‑149 (MQ=255)
                                                                                                                       cATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGc           >  3:58159/1‑149 (MQ=255)
                                                                                                                        aTGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGc                                           <  2:6621/116‑1 (MQ=255)
                                                                                                                        aTGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGc                                           >  1:6621/1‑116 (MQ=255)
                                                                                                                             ccAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTg                                                                                                                  <  6:253517/40‑1 (MQ=255)
                                                                                                                             ccAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTg                                                                                                                  >  5:253517/1‑40 (MQ=255)
                                                                                                                               aGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGCTCGTCCAc   <  4:173546/149‑1 (MQ=255)
                                                                                                                                ggCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGCTCGTCCACt  <  4:83219/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
CTGGCGCGGCGTAAAGCCGGTCAGCTGCTTGAACTGGCGGCTGAAGGCGCTGTGGTCGGTGTAGCCGCAGCGCATCGCCACTTCAGTGATCGGCAGCTCCGAATGCAGCAGGCGATGGGCATGCTCCAGGCGCGCCTTGTGGATCATCTGCCGGGGCGTGAGGTGGAACACCCGCTTGCAATAGCGCTCCAGCTGGGCCACGGAAATACCGGCAATGCGGGTCAGCTCGCTCATGCTGATCGGCTGGTGAAAATGCTGGCGAATGTGCTCGTCCACT  >  NC_002947/5221783‑5222059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑