Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,769,871 Δ1 bp 6.9% coding (1001/4716 nt) PP_5060 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,769,8710T. 6.9% 176.1 / 7.1 58coding (1001/4716 nt)PP_5060hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/23);  new base . (2/2);  total (33/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGGGCGA  >  NC_002947/5769732‑5770015
                                                                                                                                              |                                                                                                                                                
gTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACGGAACt                                                                                                                                         >  4:207288/1‑149 (MQ=255)
        tGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGCGGGGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCg                                                                                                                                    >  1:77389/1‑148 (MQ=255)
           ccTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtgg                                                                                                                                         >  4:65600/1‑134 (MQ=255)
           ccTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtgg                                                                                                                                         <  3:65600/138‑5 (MQ=255)
            cTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtg                                                                                                                                          >  1:114986/1‑133 (MQ=255)
            cTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtg                                                                                                                                          <  2:114986/136‑4 (MQ=255)
             tGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCAATGAACTCGGCGAAAAatga                                                                                                                            >  1:108806/1‑149 (MQ=255)
              gcgcTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCCACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAatcat                                                                                                                           >  7:55966/1‑149 (MQ=255)
               cgcTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAatgatg                                                                                                                          >  6:49662/1‑149 (MQ=255)
                  tGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCgg                                                                                                                       >  3:240464/1‑149 (MQ=255)
                      ctgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTAGCGACAGGCCCTTGTGAAGCGCCAGGCCCAGACCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATTAATCGGGGTc                                                                                                                   >  4:143158/1‑149 (MQ=255)
                       tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACt                                                                                                                                              <  1:205315/121‑1 (MQ=255)
                       tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACt                                                                                                                                              >  2:205315/1‑121 (MQ=255)
                       tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCg                                                                                                                  >  4:164423/1‑149 (MQ=255)
                        gtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGc                                                                                                                 >  3:41056/1‑149 (MQ=255)
                          gctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAg                                                                                                               >  5:71057/1‑149 (MQ=255)
                           ctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAg                                                                                                               <  7:19612/148‑1 (MQ=255)
                            tgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGc                                                                                                             >  3:137008/1‑149 (MQ=255)
                             gtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGc                                                                                                             >  7:31184/1‑148 (MQ=255)
                              ttGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGcc                                                                                                            <  6:71057/148‑1 (MQ=255)
                                      gCGACAAGTCGATCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                                   <  8:104706/149‑1 (MQ=255)
                                       cGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                                   >  2:189142/1‑148 (MQ=255)
                                       cGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                                   <  1:189142/148‑1 (MQ=255)
                                        gACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CTATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCagta                                                                                                 <  7:21144/149‑1 (MQ=255)
                                         aCAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGaa                                                                                                                                  >  4:101175/1‑115 (MQ=255)
                                         aCAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGaa                                                                                                                                  <  3:101175/115‑1 (MQ=255)
                                           aaGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTg                                                                                              <  5:17400/149‑1 (MQ=255)
                                              tCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTc                                                                                           >  7:56532/1‑149 (MQ=255)
                                                gAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTcc                                                                                          <  5:49662/148‑1 (MQ=255)
                                                    aTGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                     <  7:165841/149‑1 (MQ=255)
                                                         ttGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGa                                                                                >  7:32906/1‑149 (MQ=255)
                                                              ggTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTg                                                                           >  1:102947/1‑149 (MQ=255)
                                                               gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                     <  7:67721/138‑1 (MQ=255)
                                                               gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                     >  8:67721/1‑138 (MQ=255)
                                                               gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGt                                                                          >  2:198351/1‑149 (MQ=255)
                                                                  aGC‑‑GGTCTGGCTATCCGTATCGGCTCGCCACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGa                                                                       >  1:152218/1‑149 (MQ=255)
                                                                    c‑‑GGTCTGGCTATCCGTATCGGGTCGGGACAGGCCCTTGGGAAGCGACAGGCCCAGCCCACCAGTG‑GCATGGTGACTGAAGTCTTCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATc                                                                     <  8:32906/149‑1 (MQ=255)
                                                                               tATCCGTATCGGCTCGCGACAGACCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAg                                                            >  7:41520/1‑149 (MQ=255)
                                                                                 tCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAgc                                                           <  8:31184/148‑1 (MQ=255)
                                                                                   cGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAgcgc                                                         <  1:268399/148‑1 (MQ=255)
                                                                                    gTATCGGGTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAg                                                       >  3:215692/1‑149 (MQ=255)
                                                                                    gTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAg                                                       >  2:228579/1‑149 (MQ=255)
                                                                                         ggCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCgg                                                   <  8:41520/148‑1 (MQ=255)
                                                                                                   aGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTc                                        <  2:77389/149‑1 (MQ=255)
                                                                                                       ccTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAg                                     >  6:62807/1‑148 (MQ=255)
                                                                                                            ggAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCTACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGa                                <  1:198351/148‑1 (MQ=255)
                                                                                                            ggAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGa                                <  5:62807/148‑1 (MQ=255)
                                                                                                              aaGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGAttt                             >  7:27138/1‑149 (MQ=255)
                                                                                                                 cgcCAGGCCCAGCCCTCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCca                          <  4:240464/149‑1 (MQ=255)
                                                                                                                  gcCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCcag                         <  4:215692/149‑1 (MQ=255)
                                                                                                                   ccAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCcag                         >  8:49766/1‑148 (MQ=255)
                                                                                                                         ccagcccaCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAg                  >  7:112162/1‑149 (MQ=255)
                                                                                                                         ccagcccaCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAg                  >  8:106543/1‑149 (MQ=255)
                                                                                                                               caccaGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTg            <  3:164423/149‑1 (MQ=255)
                                                                                                                                accaGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTg            >  6:132305/1‑148 (MQ=255)
                                                                                                                                     tG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAgg      <  3:207288/149‑1 (MQ=255)
                                                                                                                                         cATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGGGCg   >  3:95556/1‑149 (MQ=255)
                                                                                                                                          aTGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGGGCGa  <  7:49766/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGGGCGA  >  NC_002947/5769732‑5770015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑