| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,082,642 | Δ1 bp | 6.9% | coding (392/1083 nt) | purK ← | 5‑(carboxyamino)imidazole ribonucleotide synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,082,642 | 0 | A | . | 6.9% | 166.2 / 6.2 | 58 | coding (392/1083 nt) | purK | 5‑(carboxyamino)imidazole ribonucleotide synthase |
| Reads supporting (aligned to +/- strand): ref base A (23/30); new base . (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTACACGGGCAC > NC_002947/6082494‑6082787 | gCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTACCCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGa > 6:260255/1‑149 (MQ=255) cGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATg > 2:34865/1‑149 (MQ=255) tGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCCGGCCGctgctg > 1:104207/1‑149 (MQ=255) tGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTg < 3:241655/149‑1 (MQ=255) aaCGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCGCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGc < 2:183218/149‑1 (MQ=255) cACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTg > 8:253292/1‑149 (MQ=255) cGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTgcg < 7:124518/149‑1 (MQ=255) ccTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTcc < 5:136634/149‑1 (MQ=255) cTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTccc > 6:95927/1‑148 (MQ=255) gcagGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCg < 2:108918/149‑1 (MQ=255) gCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCt > 7:242363/1‑149 (MQ=255) aTGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGc < 6:217001/149‑1 (MQ=255) cACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATg < 8:246451/115‑1 (MQ=255) cACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATg > 7:246451/1‑115 (MQ=255) cACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCgaga < 7:77289/149‑1 (MQ=255) tGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCCATGCTGGCCACGGCGGCGTGCACATAGGCCGGCGCGAGGATg > 4:158243/1‑149 (MQ=255) ccAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCg < 8:242363/149‑1 (MQ=255) ggCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGc > 1:30137/1‑126 (MQ=255) ggCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGc < 2:30137/126‑1 (MQ=255) cccACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTACCCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTggg < 2:257330/149‑1 (MQ=255) cccACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTgg < 1:26011/148‑1 (MQ=255) cccACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTgg < 7:253292/148‑1 (MQ=255) cccACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACGGCGGCGTCCAGATCGGCCAGCGAGAGGATGTAGGCGAAGGCCGGGGTtgg > 8:239697/1‑149 (MQ=255) cacGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGc < 6:79781/115‑1 (MQ=255) cacGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGc > 5:79781/1‑115 (MQ=255) acGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTcc > 1:114072/1‑102 (MQ=255) acGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTcc < 2:114072/102‑1 (MQ=255) acGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGcc < 3:210453/149‑1 (MQ=255) tGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTGCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGGGGGGTTGCCCAGGTcgc > 5:157070/1‑149 (MQ=255) gCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCgg < 8:31641/149‑1 (MQ=255) gTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGCTGCTGGCCACTGCGGCATCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTAGCGGaa > 7:142095/1‑149 (MQ=255) gcagcaCCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATg > 6:61880/1‑149 (MQ=255) cagcaCCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGaa > 8:131487/1‑144 (MQ=255) cagcaCCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGaa < 7:131487/144‑1 (MQ=255) cTTCTGGCCCTGGCCGGCGTAACCCAGGGTGCGGGGTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGGCCAGATCGGCCTGCGAGAGGATGGCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCtttt < 8:136247/149‑1 (MQ=255) tCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCAGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTc > 4:62609/1‑148 (MQ=255) cTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTc < 5:260255/147‑1 (MQ=255) gCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGGCCAGGTCGCGGAACATGCTCTTTTCGaa < 1:151835/147‑1 (MQ=255) ccTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAgg < 1:34865/149‑1 (MQ=255) cTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGc < 7:239697/149‑1 (MQ=255) ccGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTc < 3:62609/149‑1 (MQ=255) cgtcgtAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGccc < 4:152838/113‑1 (MQ=255) cgtcgtAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGccc > 3:152838/1‑113 (MQ=255) cgtAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCAc > 6:272795/1‑147 (MQ=255) tAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCACGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGCAGGCCGGGGTGGGGATGCCCAGGTCGCGGACCATGCTCTTTTCGAACAGGCGGTCACGAGc > 7:124124/1‑149 (MQ=255) ccAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCCCTGGGGCGTCCAGATCGGCCGGCGAGAGGGTGTCGGCGCAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCGCGTTTAGAACCCGCGGTCACGCGCGCTg > 6:237694/1‑149 (MQ=255) cAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGgcgc > 1:42936/1‑149 (MQ=255) gcatcggcctgcCGGCCG‑TGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTg > 6:251994/5‑149 (MQ=255) cACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGgcg < 7:211923/145‑1 (MQ=255) tcggcctgcCGGCCG‑TGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCgg < 4:157380/91‑1 (MQ=255) tcggcctgcCGGCCG‑TGCTGGCCACTGAGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCACGTCGCgg > 3:157380/2‑92 (MQ=255) aCGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTggg < 5:95927/149‑1 (MQ=255) aCGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTggg < 2:104207/149‑1 (MQ=255) ggcctgcCGGCCG‑TGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTa < 5:251994/149‑1 (MQ=255) ccggcAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACCTGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTac > 6:22615/1‑149 (MQ=255) gcAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTACACg > 1:200789/1‑149 (MQ=255) aGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTacac > 6:111480/1‑146 (MQ=255) aGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTacac < 5:111480/146‑1 (MQ=255) ccGATGCTGGCCCCTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGCAGGCCGGGGTGGGGAGGCCCAGGGCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTACACGGGCAc < 3:53732/149‑1 (MQ=255) ccGATGCTGGCCCCTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGACCCTGCGCGTGTCGAACAGGCGGGCACGAGCGATGCGCAGCGCATCGGCGCGGGGGGACCCGGGCAc > 6:133779/1‑149 (MQ=255) | GCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGCGGTCACGAGCGATGCGCAGCGATTCGGCGCTGGGGTACACGGGCAC > NC_002947/6082494‑6082787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |