| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 43,457 | Δ1 bp | 11.4% | intergenic (+23/+70) | PP_0040 → / ← cadA‑I | hypothetical protein/cadmium translocating P‑type ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 43,457 | 0 | T | . | 11.4% | 81.8 / 7.6 | 35 | intergenic (+23/+70) | PP_0040/cadA‑I | hypothetical protein/cadmium translocating P‑type ATPase |
| Reads supporting (aligned to +/- strand): ref base T (11/19); new base . (2/2); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
GTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCG > NC_002947/43319‑43600 | gTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGC‑GAT‑CAGCGCAc < 3:168703/149‑1 (MQ=255) tCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCGGAT‑A‑GCGCCCgcc > 4:105981/1‑147 (MQ=255) gTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGCCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGAccc < 1:262306/149‑1 (MQ=255) cGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCCACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGtact < 7:87113/149‑1 (MQ=255) ggTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGtactt < 8:48831/149‑1 (MQ=255) gAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGGATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGtacttctactt > 5:206201/1‑147 (MQ=255) aaaGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCAtt > 5:272774/1‑149 (MQ=255) aaaGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAA‑A‑GCGCACGCCCCGGACTTATACTTCAAGTCAtt > 8:4121/1‑149 (MQ=255) gCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTcc < 2:98268/149‑1 (MQ=255) cATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCt < 2:182803/147‑1 (MQ=255) aTTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCt > 6:123904/1‑149 (MQ=255) aTTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCt > 2:172492/1‑149 (MQ=255) ccTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTAAGTGAGGGGATTTGCCTGCTTAggt > 3:177167/1‑147 (MQ=255) aCGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCCTAATTCTAATTGAGGTGCTTTAACTGCTTAGCTTCATGATTg > 4:60220/1‑148 (MQ=255) gTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGGGCTGAT‑A‑GCGCACGGCCCGGACTTATAAGTCAGGTGATTTACCGGCTTAGGTTAAAGAATGGAGCTCAGGGATGAGGTgg > 4:43177/1‑148 (MQ=255) tGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑G‑GCGCACGCCCCGGACTTGGACTTGAGGTCATTTGCCAGCTTTGGTTGATTATTTTAGCTCAGGGTTCAGGTggg > 4:182166/1‑147 (MQ=255) cGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCtt < 3:105981/148‑1 (MQ=255) ggAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑A‑GCCCACGAACCCTACGTAAAAATAAGGTAATTTAACTGCTTATCTTGATGATTGAAGCTAAGGGATAAAGTGGttat > 4:166325/1‑147 (MQ=255) gAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTg < 6:272774/149‑1 (MQ=255) gCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAACAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTa < 3:216131/149‑1 (MQ=255) cccTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAg < 3:60220/149‑1 (MQ=255) cTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGc < 3:166325/149‑1 (MQ=255) gcgcATGGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑TCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAgg < 6:270709/149‑1 (MQ=255) aTCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATTATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGtt < 3:109684/149‑1 (MQ=255) tCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTg < 6:206201/149‑1 (MQ=255) aTCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAgac < 1:172492/149‑1 (MQ=255) gacgaGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAgacga < 5:123904/148‑1 (MQ=255) ataaaaGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGtgtg < 5:34983/147‑1 (MQ=255) gggTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCAt < 3:43177/149‑1 (MQ=255) ggTCGTGCGC‑GATAA‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTACCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATg > 2:136720/1‑149 (MQ=255) gTCGTGCGC‑GAT‑CAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGc < 7:161650/93‑1 (MQ=38) gTCGTGCGC‑GAT‑CAGCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGc > 8:161650/1‑93 (MQ=38) gTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATc < 5:59903/149‑1 (MQ=255) gTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATc < 4:177167/149‑1 (MQ=255) tGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCg > 3:35555/1‑149 (MQ=255) | GTTTCGTAGCCGGTGTCATGAGCCTCAAAGCTGCATTGGCCTGCGGCTTACGGCATGGCTCACCTGAGTGCGGAAAGGCCCTTGAAAGCCTCAAGCGCATCGGGATCGACGAGTGAGAGAAAAGTACGGGTCGTGCGCTGAT‑C‑GCGCACGACCCGTACTTCTACTTCAGGTCATTTCCCTGCTTAGCTTCATGATTGAAGCTCAGGGATCAGGTGCTTCTGCGGTAGGCCAACAGGCGCAAGGCGTTGAAGACGACCAACAGTGTGGAGCCCTCATGGATCG > NC_002947/43319‑43600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |