Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,697,896 Δ1 bp 6.9% coding (1929/2289 nt) PP_1492 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,697,8960G. 6.9% 185.0 / 6.7 58coding (1929/2289 nt)PP_1492two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (29/25);  new base . (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

CTTGCTCGATGATGGCTCGGTGGTCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCACCGACATCGACAT  >  NC_002947/1697749‑1698042
                                                                                                                                                   |                                                                                                                                                  
cTTGCTCGATGATGGCTCGGTGGTCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCtggtgg                                                                                                                                                   >  3:192101/1‑149 (MQ=255)
                   gtggtCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCg                                                                                                                                 >  7:209807/1‑148 (MQ=255)
                   gtggtCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCg                                                                                                                                 >  7:24490/1‑148 (MQ=255)
                      gtCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCGGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGGGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTAcg                                                                                                                             >  4:165814/1‑149 (MQ=255)
                        ccTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCATAcgc                                                                                                                            >  1:106303/1‑148 (MQ=255)
                         cTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGATGACTCGCTGACCGTAcgcg                                                                                                                           >  8:87784/1‑148 (MQ=255)
                          tGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGaa                                                                                                                         >  4:259101/1‑149 (MQ=255)
                                   aTGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAgcg                                                                                                                 >  3:211711/1‑148 (MQ=255)
                                    tGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAgcgc                                                                                                                >  5:183352/1‑148 (MQ=255)
                                        gAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAgct                                                                                                           >  7:160272/1‑149 (MQ=255)
                                           gACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAgctgct                                                                                                        >  5:128138/1‑149 (MQ=255)
                                             cTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTgg                                                                                                      <  6:145838/149‑1 (MQ=255)
                                             cTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTgg                                                                                                      <  3:181794/149‑1 (MQ=255)
                                                gCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCCCATCGCGCGCGGCAGCAGCGGAGCCCTTGGCGTAGTCCGCAACCGCATCCTCGTGGTCGACCACTCGCTGCCCGTACGCGAACTGCAGCTCCAGCTGCTGGGc                                                                                                    >  1:49752/1‑148 (MQ=255)
                                                       tcgctGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTgg                                                                                            >  6:175074/1‑149 (MQ=255)
                                                             gAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTac                                                                                        <  6:128138/147‑1 (MQ=255)
                                                              aaaaaCTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTacgac                                                                                     <  2:171442/149‑1 (MQ=255)
                                                                   cTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACgtggc                                                                                <  8:24490/149‑1 (MQ=255)
                                                                   cTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACgtggc                                                                                <  8:209807/149‑1 (MQ=255)
                                                                      cTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACgtggcggt                                                                             <  5:175074/149‑1 (MQ=255)
                                                                         aGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACgtggcggtg                                                                            <  3:219230/147‑1 (MQ=255)
                                                                          gCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACgtggcggtggcg                                                                         >  2:118632/1‑149 (MQ=255)
                                                                           cACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGtggtggtggcgg                                                                        <  8:133030/149‑1 (MQ=255)
                                                                           cACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGtggcggtggcgg                                                                        <  4:132453/149‑1 (MQ=255)
                                                                            aCGGGCAGCCTGGAGCGCATCGGGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTggcggtggcggt                                                                       <  3:165814/149‑1 (MQ=255)
                                                                                      tGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGCCGACTCGCTGACCGTACGAGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATg                                                             >  1:25641/1‑149 (MQ=255)
                                                                                       ggAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATg                                                             >  3:156040/1‑148 (MQ=255)
                                                                                          gcgcATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTacg                                                                                       >  3:57495/1‑119 (MQ=255)
                                                                                          gcgcATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTacg                                                                                       <  4:57495/119‑1 (MQ=255)
                                                                                          gcgcATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACg                                                         >  1:13866/1‑149 (MQ=255)
                                                                                          gcgcATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACg                                                         >  1:9507/1‑149 (MQ=255)
                                                                                             cATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCt                                                      >  5:189909/1‑149 (MQ=255)
                                                                                             cATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCt                                                      <  1:118632/149‑1 (MQ=255)
                                                                                              aTCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTg                                                     >  2:4841/1‑149 (MQ=255)
                                                                                               gcGAGCGCTGCAGCAGCTGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGGGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTgg                                                    <  2:49752/148‑1 (MQ=255)
                                                                                                 gAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGTGGTGGCGGTGGATGGCATGGACGGCTGGaa                                                  <  4:192101/149‑1 (MQ=255)
                                                                                                 gAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGaa                                                  >  6:8601/1‑149 (MQ=255)
                                                                                                 gAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGa                                                   <  3:259101/148‑1 (MQ=255)
                                                                                                          agcagcGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGc                                               <  4:94480/143‑1 (MQ=255)
                                                                                                          agcagcGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGc                                               >  3:94480/1‑143 (MQ=255)
                                                                                                            cagcGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCa                                       <  1:4841/149‑1 (MQ=255)
                                                                                                             agcGGAGCCCGTGGCGTAGTCCGCAAACGCTTACTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAAAGACCTGCGCAg                                      >  5:33094/1‑149 (MQ=255)
                                                                                                             agcGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATg                                                             <  8:192905/126‑1 (MQ=255)
                                                                                                             agcGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATg                                                             >  7:192905/1‑126 (MQ=255)
                                                                                                               cGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTg                                    <  5:8601/149‑1 (MQ=255)
                                                                                                                 gAGCCCGGGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAg                                  <  6:189909/149‑1 (MQ=255)
                                                                                                                    cccGTGGCGTAGTCCGCAAACGCATCCTGGT‑GTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTggcggtggcggt                                                                       <  1:6535/108‑1 (MQ=255)
                                                                                                                    cccGTGGCGTAGTCCGCAAACGCATCCTGGT‑GTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTggcggtggcggt                                                                       >  2:6535/1‑108 (MQ=255)
                                                                                                                    cccGTGGCGTAGTCCGCAAACGCATCCTGGT‑GTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTggcggtggcggt                                                                       >  2:2255/1‑108 (MQ=255)
                                                                                                                    cccGTGGCGTAGTCCGCAAACGCATCCTGGT‑GTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTggcggtggcggt                                                                       <  1:2255/108‑1 (MQ=255)
                                                                                                                              aGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGATGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGAGAGGACTTCGACCTGt                     >  2:91975/1‑149 (MQ=255)
                                                                                                                               gTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGtt                    <  4:156040/149‑1 (MQ=255)
                                                                                                                               gTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGt                     <  1:33421/148‑1 (MQ=255)
                                                                                                                                tCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGtt                    >  7:131648/1‑148 (MQ=255)
                                                                                                                                     aaaCGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCAc              <  8:160272/149‑1 (MQ=255)
                                                                                                                                     aaaCGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCAc              >  4:210546/1‑149 (MQ=255)
                                                                                                                                     aaaCGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCAc              >  4:214154/1‑149 (MQ=255)
                                                                                                                                         gCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACTCCCTGCGCAGt                                     <  4:206777/122‑1 (MQ=255)
                                                                                                                                         gCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGt                                     >  3:206777/1‑122 (MQ=255)
                                                                                                                                               tggtggTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCCCcgacatcga     >  5:197977/1‑148 (MQ=255)
                                                                                                                                                 gtggtCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCACcgacatcgacat  <  4:211711/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTGCTCGATGATGGCTCGGTGGTCCTGATCGTCGATGTCGAAGACTTGCTACGCTCGCTGGAAAAACTGCTCAGCACGGGCAGCCTGGAGCGCATCGAGCGCGGCAGCAGCGGAGCCCGTGGCGTAGTCCGCAAACGCATCCTGGTGGTCGACGACTCGCTGACCGTACGCGAACTGCAGCGCAAGCTGCTGGGCAACCGTGGCTACGACGTGGCGGTGGCGGTGGATGGCATGGACGGCTGGAACGCCCTGCGCAGTGAGGACTTCGACCTGTTGATCACCGACATCGACAT  >  NC_002947/1697749‑1698042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑