| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,437,203 | Δ1 bp | 9.3% | coding (123/2148 nt) | fadB → | enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,437,203 | 0 | C | . | 9.3% | 130.5 / 7.3 | 43 | coding (123/2148 nt) | fadB | enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (20/19); new base . (2/2); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01 | |||||||||||
TTCCATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCTGCCTGAGGCCGAACTGGTCGCTGGCAACCTGG > NC_002947/2437058‑2437351 | ttCCATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAg > 7:144233/1‑149 (MQ=255)ttCCATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAg < 8:144233/149‑1 (MQ=255)ttCCATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAg > 1:72164/1‑149 (MQ=255) ccATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAg < 1:253363/147‑1 (MQ=255) gTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGAt > 1:10283/1‑149 (MQ=255) cAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCG‑CAGGCCGTCGAt < 2:139366/140‑1 (MQ=255) cAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCG‑CAGGCCGTCGAt < 2:143813/140‑1 (MQ=255) cAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCG‑CAGGCCGTCGAt > 1:143813/1‑140 (MQ=255) cAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCG‑CAGGCCGTCGAt > 1:139366/1‑140 (MQ=255) aGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCg < 8:98620/148‑1 (MQ=255) ttGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGc < 7:190918/149‑1 (MQ=255) ttGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGc < 2:72164/149‑1 (MQ=255) gatgatTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGcc > 6:261636/1‑148 (MQ=255) atTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGc > 2:1880/1‑149 (MQ=255) aCGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACAACAAGGGTGAGTCCGTCAACAAGTTCAACACCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATTCCATCCGGGCCGATGATTCg > 7:117659/1‑149 (MQ=255) gAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGcc < 8:135351/120‑1 (MQ=255) gAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGcc > 7:135351/1‑120 (MQ=255) gAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGt < 6:258443/122‑1 (MQ=255) gAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGt > 5:258443/1‑122 (MQ=255) gAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGt > 6:151860/1‑149 (MQ=255) aaaGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAggg > 3:66981/1‑149 (MQ=255) aaGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGc < 2:10283/149‑1 (MQ=255) aCGGGTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCGTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGt < 8:180725/149‑1 (MQ=255) gTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAg < 5:261636/149‑1 (MQ=255) gTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAg < 1:1880/149‑1 (MQ=255) ctctTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTc > 2:167103/1‑125 (MQ=255) ctctTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTgg > 8:24463/1‑148 (MQ=255) tctTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGc > 3:81407/1‑148 (MQ=255) aGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACgt < 5:151860/149‑1 (MQ=255) gCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTc < 5:157656/149‑1 (MQ=255) tcgtcgAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCATGATCGTCAGGAGTGGCAAGGACGTGTTCAtc < 4:66981/149‑1 (MQ=255) gtcgAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCAtcgt > 2:15229/1‑149 (MQ=255) tcgAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCAtcgtc > 3:233700/1‑149 (MQ=255) tCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAg > 5:2327/1‑149 (MQ=255) gTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTtcgtc < 4:81407/149‑1 (MQ=255) gTCCGTCAACAAGTTCAACCGCCTAACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGAc > 6:20411/1‑148 (MQ=255) caaGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGa < 2:42341/129‑1 (MQ=255) aaGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCt > 2:127100/1‑149 (MQ=255) gTTCAACCGCCTTACCCGGAAGGATGTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGTCGTGAGCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGTCGCCGACATCACCGAGTTCGGCGACAACTTCAAGCTGc < 8:117659/149‑1 (MQ=255) ccGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCAtc > 2:153021/1‑100 (MQ=255) ccGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGAACGTCAGGAGTGGCAAGGACGTGTTCAtc < 1:153021/100‑1 (MQ=255) aCCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCTGCCTGAGGCCGaa < 1:15229/147‑1 (MQ=255) atGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCAtcgtcg > 4:53505/2‑85 (MQ=255) gcCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCTGCCTGAGGCCGAACTGGTCGCTGTCAACCTg < 1:127100/149‑1 (MQ=255) ccAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCTGCCTGAGGCCGAACTGGTCGCTGGCAACCTgg < 7:24463/149‑1 (MQ=255) | TTCCATATCGTGGAGATCAGTTGATGATTTACGAAGGTAAAGCCATCACGGTTAAGGCTCTTGAAAGTGGCATCGTCGAGCTCAAGTTCGACCTCAAGGGTGAGTCCGTCAACAAGTTCAACCGCCTTACCCTGAACGAGCTGCGCCAGGCCGTCGATGCCATCCGGGCCGATGCTTCGGTCAAGGGCGTGATCGTCAGGAGTGGCAAGGACGTGTTCATCGTCGGCGCCGACATCACCGAGTTCGTCGACAACTTCAAGCTGCCTGAGGCCGAACTGGTCGCTGGCAACCTGG > NC_002947/2437058‑2437351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |