Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,755,091 Δ1 bp 10.0% coding (1417/3147 nt) czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,755,0910G.10.0% 116.9 / 7.9 40coding (1417/3147 nt)czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (19/16);  new base . (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGG  >  NC_002947/2754956‑2755238
                                                                                                                                        |                                                                                                                                                    
cACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCaaagatcg                                                                                                                                         <  1:142005/149‑7 (MQ=25)
          tGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATg                                                                                                                              >  3:134825/1‑149 (MQ=255)
           gCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑g                                                                                                                                             <  6:222553/133‑1 (MQ=255)
           gCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑g                                                                                                                                             >  5:222553/1‑133 (MQ=255)
             tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGcc                                                                                                                           >  5:172677/1‑149 (MQ=255)
             tGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGcc                                                                                                                           >  7:132790/1‑149 (MQ=255)
                  cgcgGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTAc                                                                                                                      >  1:240259/1‑149 (MQ=255)
                      gcgAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTg                                                                                                                  <  6:165688/149‑1 (MQ=255)
                                    gAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCGTTACCGTGGTCATGGCCCTGtt                                                                                                    >  5:241546/1‑149 (MQ=255)
                                    gAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGACTTTACCGTGGTCATGGCCCTGtt                                                                                                    >  8:247208/1‑149 (MQ=255)
                                     aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GAt                                                                                                                                           >  6:202100/1‑109 (MQ=255)
                                     aGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GAt                                                                                                                                           <  5:202100/109‑1 (MQ=255)
                                        tgtTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgc                                                                                                <  6:172677/149‑1 (MQ=255)
                                        tgtTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGgc                                                                                                <  8:132790/149‑1 (MQ=255)
                                               cgatgaacgcgAAGCACGCCATCCGCTGATATAACTTCAGTAGATCATCATGGAGGTCTACCTGCAGATATTTGAACT‑GCCCGGGGTCTAGGGAAA‑GATGTTACAACATATGGCCTTTACAGTCATCATGGCCCTGTTGGGCGCgagga                                                                                         >  4:241971/8‑149 (MQ=255)
                                                gCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCgatgat                                                                                        <  4:134825/149‑1 (MQ=255)
                                                     ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCa                                                                                                                                                <  4:92982/89‑1 (MQ=18)
                                                     ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCTCGACCCCGGTC‑AGGGCa                                                                                                                                                >  3:92982/1‑89 (MQ=18)
                                                     ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAACGATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                           <  1:176779/126‑1 (MQ=255)
                                                     ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATgg                                                                                                           >  2:176779/1‑125 (MQ=255)
                                                     ccGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCttt                                                                                   >  5:189971/1‑149 (MQ=255)
                                                      cgcgAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTc                                                                                  >  4:212836/1‑149 (MQ=255)
                                                          aaGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTc                                                                              <  6:259289/149‑1 (MQ=255)
                                                                                  ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGcc                                                      <  2:240259/149‑1 (MQ=255)
                                                                                  ggCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCACTGATGGGCGCTATGATACTTTCGGTAACGTTCGTTCAGGCAGACATTGcc                                                      >  4:199679/1‑149 (MQ=21)
                                                                                         gATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTc                                                <  7:247208/148‑1 (MQ=255)
                                                                                           tcatcaTGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGt                                              >  4:229349/1‑148 (MQ=255)
                                                                                                    gggtgTCCCTGCCGATCTGTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAg                                    <  6:189971/148‑1 (MQ=255)
                                                                                                           ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCAtt                                                         >  4:3448/1‑121 (MQ=255)
                                                                                                           ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCAtt                                                         <  3:3448/121‑1 (MQ=255)
                                                                                                           ccTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAag                              <  3:212836/148‑1 (MQ=255)
                                                                                                                cGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGGTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGACCTGTTCGTCACCGGCAAGGTCAaggaagag                        >  7:59914/1‑149 (MQ=255)
                                                                                                                 gATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagag                        >  5:109508/1‑148 (MQ=255)
                                                                                                                  aTCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAaggaagagg                       >  3:180352/1‑148 (MQ=255)
                                                                                                                    tcttGCCCTCGACCCCGGTC‑AGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCACGGTCAaggaagaggaag                    >  1:122073/3‑149 (MQ=255)
                                                                                                                            t‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGt             >  1:100176/1‑148 (MQ=255)
                                                                                                                               agcGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCTCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCTCCGGCAAGGTCAAGGAGGAGGCAGGCCTGGTGAt          <  3:241971/147‑1 (MQ=255)
                                                                                                                               aCCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCCTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGAt          <  6:241546/149‑1 (MQ=255)
                                                                                                                                    gtgcGAGGGCAA‑TATGTTCCACCCTATGGCCTTTACCGTCGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGAAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTa     <  3:199679/146‑1 (MQ=255)
                                                                                                                                       cGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTacgg  >  7:107804/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
CACGGTCGCATGCTGACCCGCGGCGAACGGTTCCATGAGGTGTTTGCCGCTGCCCGCGAAGCACGCCGGCCGCTGATCTATGGGCAGTTGATCATCATGGTGGTGTACCTGCCGATCTTTGCCCT‑GACCGGGGTCGAGGGCAA‑GATGTTCCACCCTATGGCCTTTACCGTGGTCATGGCCCTGTTGGGCGCGATGATCCTTTCGGTCACGTTCGTTCCGGCAGCCATTGCCCTGTTCGTCACCGGCAAGGTCAAGGAAGAGGAAGGCCTGGTGATGCGTACGG  >  NC_002947/2754956‑2755238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑