Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,549,191 Δ1 bp 10.0% intergenic (+63/‑73) pydB → / → pydX bifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,549,1910G.10.0% 152.8 / 10.9 50intergenic (+63/‑73)pydB/pydXbifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base . (2/3);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

AACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCT  >  NC_002947/4549049‑4549337
                                                                                                                                               |                                                                                                                                                   
aaCGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCtgtg                                                                                                                                               <  3:211870/149‑1 (MQ=255)
      cggcGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCg                                                                                                                                         <  6:194059/149‑1 (MQ=38)
          gTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGccc                                                                                                                                     <  3:186470/149‑1 (MQ=255)
              ccGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAg                                                                                                                                  >  7:194500/1‑148 (MQ=255)
                     tgtATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGAcc                                                                                                                          <  4:57697/149‑1 (MQ=255)
                         tGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCaa                                                                                                                      >  8:101980/1‑149 (MQ=255)
                                cTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGt                                                                                                               >  8:226274/1‑149 (MQ=255)
                                   gggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGc                                                                                                            <  2:31264/149‑1 (MQ=255)
                                    ggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGcc                                                                                                           >  8:148970/1‑149 (MQ=255)
                                       gcCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGc                                                                                                        <  7:80924/149‑1 (MQ=255)
                                         cGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCAc                                                                                                      >  2:197977/1‑149 (MQ=255)
                                            gCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCa                                                                                                    >  2:135944/1‑148 (MQ=255)
                                             ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc                                                                                                                                       <  7:205949/112‑1 (MQ=25)
                                             ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc                                                                                                                                       >  8:205949/1‑112 (MQ=25)
                                                       gCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATAACTGTCGCCTGCACCAAAAAAAATa                                                                                           >  6:164671/1‑146 (MQ=255)
                                                          gCCGATCCCGGTTCCGCGCTGACGTCATTGGGGCTGCGGTGCAGCCCAGCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAgg                                                                                     <  4:169720/149‑1 (MQ=39)
                                                             gATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTa                                                                                  <  7:101980/149‑1 (MQ=255)
                                                               tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                                           >  1:204009/1‑138 (MQ=255)
                                                               tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                                           <  2:204009/138‑1 (MQ=255)
                                                                  cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga                                                                                       >  5:245123/1‑139 (MQ=255)
                                                                  cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga                                                                                       <  6:245123/139‑1 (MQ=255)
                                                                        cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGAc                                                                       <  5:164671/148‑1 (MQ=255)
                                                                        cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGATAC‑AACCGTGAc                                                                       >  6:213843/1‑148 (MQ=255)
                                                                           gcTGAAGTCATTGGGGCTGCTGTGCAGCCAATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTTTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAAc                                                                   <  1:124196/149‑1 (MQ=255)
                                                                             tGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCAGTCGCCTGCACCAAAAAAAATATAGAGGCTAC‑AAACGTGACCAACGc                                                                 >  8:56695/1‑149 (MQ=255)
                                                                              gAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATag                                                                                          <  8:60622/124‑1 (MQ=255)
                                                                               aaGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGccc                                                               >  6:203332/1‑149 (MQ=255)
                                                                                aGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCt                                                              <  5:249271/149‑1 (MQ=255)
                                                                                   cATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCCCCAAAAAAAATAGAGAGGCTAC‑AACCGTGCCCAACGCCCTGac                                                           >  3:22860/1‑148 (MQ=255)
                                                                                    aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                                           <  7:97981/117‑1 (MQ=255)
                                                                                    aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                                           >  8:97981/1‑117 (MQ=255)
                                                                                    aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGa                                                            <  7:148970/147‑1 (MQ=255)
                                                                                    aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAAc                                                          >  2:192198/1‑149 (MQ=255)
                                                                                        gtgctgctGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAt                                                       <  7:56695/146‑1 (MQ=255)
                                                                                         ggCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAttt                                                     <  1:197977/149‑1 (MQ=255)
                                                                                                  gCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGcccc                                           >  4:251810/1‑149 (MQ=255)
                                                                                                      cccATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGCCCCCggg                                       <  3:251810/149‑1 (MQ=255)
                                                                                                         aTCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGcc                                     >  6:171349/1‑149 (MQ=255)
                                                                                                          tCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTTGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGATCCATTTGCCGCGCCCCCGGGCCg                                    <  7:161928/149‑1 (MQ=255)
                                                                                                            gcgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCgg                                   <  7:226274/148‑1 (MQ=255)
                                                                                                             cgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGgc                                  <  1:192198/149‑1 (MQ=255)
                                                                                                                   aaGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc                            >  3:97561/1‑148 (MQ=255)
                                                                                                                      gCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGt                        <  1:135944/149‑1 (MQ=255)
                                                                                                                         gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc                            <  7:43984/142‑1 (MQ=38)
                                                                                                                         gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc                            >  8:43984/1‑142 (MQ=38)
                                                                                                                            cccACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGcc                   >  4:227274/1‑148 (MQ=255)
                                                                                                                               acaAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGCCCAGTTGGCCGAgc               >  4:5855/1‑149 (MQ=255)
                                                                                                                                   g‑gACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCtt           >  4:25243/1‑149 (MQ=255)
                                                                                                                                     gACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑ACCCGTGACCAACGCCCTGACCCATTTGCCGCGCCCCCGGGCCGGCGCCGCCCAGTTGGCCGAGCGCtt           >  7:239487/1‑148 (MQ=255)
                                                                                                                                             tCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCt  <  4:97561/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
AACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTTCAGCGACCT  >  NC_002947/4549049‑4549337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑