| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,740,805 | Δ1 bp | 100% | intergenic (+42/+711) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,805 | 0 | T | . | 91.2% | 124.6 / 4.6 | 34 | intergenic (+42/+711) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (19/12); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTG‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGG > NC_002947/4740661‑4740948 | cTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGCTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCGAGGGCAGCcttctt < 1:143061/149‑1 (MQ=255) gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat > 8:48804/1‑147 (MQ=255) gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCCGCCCTCGGGCAGCCttttttat < 7:41632/149‑3 (MQ=255) tccagtgcgagatccagcccGTACAAGAGCGGCAGTCCGCGCCTGCGGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCATGTAAGCTGCAGTAGCCGAACGCAAGCAGGCTGCCCTCGGGCAGccttttttat < 4:47223‑M2/128‑3 (MQ=255) aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGTCTGCCCTCGGGCAGCCttttttatt < 5:164356/149‑4 (MQ=255) gaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgt < 5:230714/149‑7 (MQ=255) aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc > 4:27703/1‑142 (MQ=255) aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtc > 3:259107/1‑142 (MQ=255) tccagcccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAAAAGGCTGCCCGAGGGCAGCcttctt > 8:19732‑M2/9‑133 (MQ=255) tccagcccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAAAAGGCTGCCCGAGGGCAGCcttctt < 7:19732‑M2/125‑1 (MQ=255) cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCctc > 4:150675‑M2/4‑149 (MQ=255) aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCt > 5:272339/1‑149 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat < 1:67042/112‑3 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttat > 2:67042/1‑110 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGa < 3:5260/149‑1 (MQ=255) cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCcgcg > 1:162942/1‑147 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgggcaaggc < 7:142200/142‑8 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag < 5:276343/149‑1 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACAGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgggcaaggc > 8:142200/1‑135 (MQ=255) gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag > 5:161064/1‑149 (MQ=255) aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc < 2:16883/149‑1 (MQ=255) acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgg < 8:44293/124‑1 (MQ=255) cacCGCCGACCAGACCCGCGACATCGTTCCCCTTAAAGCCCCCAAGTACGCTGCATTAGCCGACCGCAAGAAGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCT‑GGGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGTGGCCGGcaca < 4:196404/148‑1 (MQ=255) cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCACAAACCGGCCATGCCCGCCAAGAGGCCGCcacacaca > 1:244794/1‑149 (MQ=255) taaGCGTGACTTCGTTGCCCTTAAATACCGAATTTATGCTGCAGTAGCGTAACGAAATATGGGTGCCCT‑GGGTAAGCTTTTTTTTTTTGTCGT‑GTGCGGGCCTTTGGGGGGCTTGGCCCGCTATGTGGCCGGCACAGACACCACAAAAc < 2:191120/148‑1 (MQ=255) cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACCGACACCACAAACCCTCAGgttttat > 8:132394/1‑147 (MQ=255) gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCTAACGCAAGAAGGCTGCCGTCGGG‑ATGCGTTTTTTATTTTTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct < 7:132394/148‑1 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga < 4:132404/109‑1 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga > 3:132404/1‑109 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTTGCCGCGAAGAGGCCGGCACAGAAACCACAAAAACACAGTTTTtctagatag > 7:8265/1‑142 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc > 2:41262/1‑146 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc < 1:41262/146‑1 (MQ=255) tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCCCAGCCACCACAAAACCTCAGGTTTTCTccgcccgg > 6:215386/1‑148 (MQ=255) aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCAGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc > 1:115554/1‑149 (MQ=255) gCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGc < 6:272339/149‑1 (MQ=255) gCTGCAGTAGCCTAACGCAAGAAGGCTGCCCGCGGGCAGCC‑TTTTTTATTTTGCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg < 5:91902/149‑1 (MQ=255) cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 7:48804/149‑1 (MQ=255) aaCCCAAGACGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCT‑GTCCCGGCCTGTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc < 3:27703/149‑1 (MQ=255) aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACAACAGTTTTTATAAGCCCGCTCACGCCGCCCTTTAAg > 2:170877/1‑131 (MQ=37) aaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGGTTTTCTCCGCCCGCTCCCGCAGCCCTTTAAggg > 7:13920/4‑133 (MQ=37) agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCGCAGCAACCACAAAACCGCAGTTTTTATACGCCCGCGCCCGCAGCCCAGTCAGGGGATTGTAAGgcgccgcc > 4:237043/1‑145 (MQ=255) gaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCACTTAGCGGCCTTGCCAGCGAAGAGGCCGGCACACACACCACAAAACCGAAGTTTTTATCCGCCCCCTCACGCAACCCATTCAGGGTATTGAAAGGCGCATcca > 7:37394/1‑149 (MQ=255) tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGCCACCACAAAACCTCAGTGTTTCTCCGCCCGCGCCCGCCCCCCGTTCAGGGGTATGACCGGCGCATCcaccacta > 8:246562/1‑147 (MQ=255) cccgagggCAGCC‑TTCTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGGGCAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCTTTAAGGGTATTGAACGGCGCATCCCCCACGAAc > 3:43366/6‑149 (MQ=255) cccTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc < 2:162942/149‑1 (MQ=255) gggcatgtttttttATTTTTCCGTGTGCTGCCCTTTGCGGGGCTTGGCCGGCTATGCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt < 2:244794/140‑1 (MQ=255) gCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTgg > 3:95010/1‑149 (MQ=255) | CTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTG‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGG > NC_002947/4740661‑4740948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |