| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 362,755:1 | +G | 11.8% | coding (728/966 nt) | lcdH ← | L‑carnitine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 362,755 | 1 | . | G | 11.8% | 171.8 / 20.8 | 51 | coding (728/966 nt) | lcdH | L‑carnitine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (23/22); new base G (4/2); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01 | |||||||||||
GATGCTGCGCTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAG > NC_002947/362613‑362900 | gATGCTGCGCTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCG‑ATGCCGGCATGCGCCAc < 7:237220/149‑1 (MQ=255) tgctgcGCTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCg < 6:115613/149‑1 (MQ=255) gcTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAgg < 6:128229/149‑1 (MQ=255) gcTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAgg > 5:128229/1‑149 (MQ=255) tCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGaa > 7:53365/1‑149 (MQ=255) cGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAAt > 6:24194/1‑149 (MQ=255) ggAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAAt < 7:78684/148‑1 (MQ=255) gAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAAtgt < 2:160796/149‑1 (MQ=255) aGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGc < 2:108784/149‑1 (MQ=255) aGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgc < 6:266491/149‑1 (MQ=255) gCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgcg > 2:243554/1‑149 (MQ=255) tCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCaaa < 1:43521/149‑1 (MQ=255) tCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgcgc > 3:9248/1‑141 (MQ=255) tCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgcgc < 4:9248/141‑1 (MQ=255) aGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGGGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAAt < 3:98943/149‑1 (MQ=255) ggtCCGGTGCTGGCAGGTACGTCCCGGGCAGCTTCAGGGCGGGGCCGAACTGGCACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATc < 8:132661/147‑1 (MQ=255) cGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGcgt > 1:268580/1‑149 (MQ=255) gCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgcgc < 6:18869/126‑1 (MQ=255) gCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTgcgc > 5:18869/1‑126 (MQ=255) gCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGAtt > 3:234767/1‑149 (MQ=255) gTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGc > 3:144838/1‑149 (MQ=255) cGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGccc < 4:144838/148‑1 (MQ=255) gTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGACCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCgt > 8:119686/1‑149 (MQ=255) tCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCGCATGAACTAACAGAGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCGCgtg > 6:209780/1‑149 (MQ=255) ccAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCgtgg > 2:20555/1‑149 (MQ=255) ttCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTcc > 6:56619/1‑149 (MQ=255) ttCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTcc > 5:264082/1‑149 (MQ=255) cAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCcgt > 6:20047/1‑149 (MQ=255) gggCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCcgtcg < 8:53365/149‑1 (MQ=255) tgtgACATGAAGTGGCGCATGCCGGCGT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGc > 1:140622/1‑147 (MQ=255) tgtgACATGAAGTGGCGCATGCCGGCCT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGc > 1:145016/1‑147 (MQ=255) tgtgACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGc > 3:247789/1‑147 (MQ=255) tgACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCaa < 3:134053/147‑1 (MQ=255) tgACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAgg < 1:20555/149‑1 (MQ=255) aCATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGtt < 6:167263/133‑1 (MQ=255) aCATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGtt > 5:167263/1‑133 (MQ=255) aCATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGc < 5:20047/148‑1 (MQ=255) aTGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATcgcg < 6:256382/96‑1 (MQ=255) aTGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATcgcg > 5:256382/1‑96 (MQ=255) ccggTGGCG‑ATGCCGGCATGCGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGCCCAGGTGCAAGGCCTcgc > 6:124622/4‑149 (MQ=255) ccggTGGCG‑ATGCCGGCATGCGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTcgc > 6:223486/4‑149 (MQ=255) cggTGGCG‑ATGCCGGCATGCGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTcgcg > 4:141407/3‑149 (MQ=255) tggcgaTGCCGGCATGCGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACt < 7:44638/112‑1 (MQ=255) tggcgaTGCCGGCATGCGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACt > 8:44638/6‑117 (MQ=255) gcgcATGCCGGCAT‑CGCCACCGGCCAGGGTATACGTAATGAATGTTCCCATGAACGACCATGGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCaga < 6:264082/149‑1 (MQ=255) cgcATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCagag < 1:243554/149‑1 (MQ=255) aTGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAgcgc < 2:268580/149‑1 (MQ=255) gCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCtt > 6:37583/1‑149 (MQ=255) gCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCtt > 6:41725/1‑149 (MQ=255) cGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCg < 5:24194/149‑1 (MQ=255) gCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAg > 6:180092/1‑149 (MQ=255) | GATGCTGCGCTCGCCCTGTTGCTCGGAAGTGCCGCTCACCACATCGTCGATCAGCTTGTCGGTCAGTTCCGGTGCTGGCAGGTACGTCCAGGGCAGCTTCAGGGCTGGGCCGAACTGTGACATGAAGTGGCGCATGCCGGCAT‑CGCCACCGGCCAGGGTATAGGTAAGGAATGTGCCCATGAACGACCAGCGCAAGCCTGCGCCAAAGCGAATCGCGTCGTCGATTTCGCCCGTGGTGGCAACTCCGTCGTTGACCAGGTGCAAGGCCTCGCGCCAGAGCGCTTCGAG > NC_002947/362613‑362900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |