Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,674,750:1 +G 100% intergenic (+112/+28) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7501.G98.4% 205.3 / ‑3.8 61intergenic (+112/+28)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (27/33);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674605‑5674888
                                                                                                                                                   |                                                                                                                                          
tGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAg‑c‑ggc                                                                                                                                         <  7:62393/149‑3 (MQ=255)
    cgcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAg‑c‑ggccccc                                                                                                                                     >  6:16166/1‑143 (MQ=255)
     gcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAg‑c‑ggccccct                                                                                                                                    >  3:76239/1‑142 (MQ=255)
        cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCgg                                                                                                                                  <  7:56541/149‑1 (MQ=255)
                ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCa                                                                                                                            <  1:141004/147‑1 (MQ=255)
                ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGc                                                                                                                          <  4:76239/149‑1 (MQ=255)
                ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTAGCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCa                                                                                                                            <  7:196554/147‑1 (MQ=255)
                         aaGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTc                                                                                                                             <  2:205349/137‑1 (MQ=255)
                         aaGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAAGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTc                                                                                                                             >  1:205349/1‑137 (MQ=255)
                          aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                <  2:31855/149‑1 (MQ=255)
                            ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTa                                                                                                              <  5:74911/149‑1 (MQ=255)
                                          ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCCCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGGGGCCTAGTGTCGTGATAGGGCCGCAAAGAG‑GCCCCCACGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCacac                                                                                                >  2:242267/1‑149 (MQ=38)
                                           cGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                               >  6:213462/1‑149 (MQ=255)
                                             aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                             >  2:132739/1‑149 (MQ=255)
                                                  gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                            <  8:74075/145‑1 (MQ=255)
                                                  gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                          >  7:74075/1‑147 (MQ=255)
                                                    tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTAGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAC‑CGGCCCCCACGGTTTTCAGCTGGCAGAAATTAATTCTGCTTCACACCTACGacaat                                                                                      >  8:99572/1‑147 (MQ=37)
                                                                cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtc                                                                          >  4:195910/1‑149 (MQ=255)
                                                                   tCACGACACAAGGCCGCTCCTGCAGAGCATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                       >  7:64123/1‑149 (MQ=255)
                                                                     aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  5:16166/148‑1 (MQ=255)
                                                                      cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                     >  8:185752/1‑148 (MQ=255)
                                                                      cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                     >  4:44044/1‑148 (MQ=255)
                                                                      cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                    >  2:187199/1‑149 (MQ=255)
                                                                        acacAAAGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                  <  4:116613/149‑1 (MQ=255)
                                                                            aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                >  5:265679/1‑131 (MQ=255)
                                                                            aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                <  6:265679/131‑1 (MQ=255)
                                                                            aaGGCCGCTACTGCAGAGAATTTATTGATCTATACAGGAGCGGCCGTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCGCCGTTTTTAGCTGGCAGAAATTACTTCTGCTTGACACCTTCGACCCTGATATGCAGGTCCAGGGTCTggg                                                               >  4:179146/1‑146 (MQ=255)
                                                                             aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                             <  8:64123/149‑1 (MQ=255)
                                                                                      cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGATGGCAGAAATTACTTATGCTTCAAACCTTCGACACTGATATCCAGGTCCAGGGTATGCGAAGTAGCAccc                                                    >  5:3009/1‑149 (MQ=38)
                                                                                         cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCggg                                                 >  4:263591/1‑149 (MQ=255)
                                                                                         cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                  <  8:171781/148‑1 (MQ=255)
                                                                                         cAGAGAATTTCTGGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCACCTCGGTTTTCAGCTGGCAGAAATTAATTCTGCTTCACACCTTCGACACAGATATCCAGGTCAAGGGTCTGCGAAGTAGCACCCtgg                                                 >  4:228569/1‑149 (MQ=38)
                                                                                            agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                              <  5:213462/149‑1 (MQ=255)
                                                                                            agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCt                                              >  5:273214/1‑149 (MQ=255)
                                                                                              aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                            >  2:20360/1‑149 (MQ=255)
                                                                                               aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                             >  4:204995/1‑83 (MQ=25)
                                                                                               aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                             <  3:204995/83‑1 (MQ=25)
                                                                                               aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                            <  1:132739/148‑1 (MQ=255)
                                                                                                tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTTAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                          <  3:179146/149‑1 (MQ=255)
                                                                                                  tCTTGCTCTCTACCGGACCGGCCTTGTGTCGTGATATGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                         <  6:3009/148‑1 (MQ=255)
                                                                                                    ttGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTg                                           <  8:244443/144‑1 (MQ=255)
                                                                                                    ttGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTg                                           >  7:244443/1‑144 (MQ=255)
                                                                                                      gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                     <  1:171833/148‑1 (MQ=255)
                                                                                                      gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                     <  4:232085/148‑1 (MQ=255)
                                                                                                        tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                  >  1:143137/1‑149 (MQ=255)
                                                                                                          tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                <  7:99572/149‑1 (MQ=255)
                                                                                                            tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  5:144317/144‑1 (MQ=255)
                                                                                                              cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttc                            <  2:202048/149‑1 (MQ=255)
                                                                                                                 gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         <  7:185752/149‑1 (MQ=255)
                                                                                                                 gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         <  1:187199/149‑1 (MQ=255)
                                                                                                                  aGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                        >  6:63722/1‑149 (MQ=255)
                                                                                                                   gCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGCAGTAGCACCCGGGCCTGTGATGCCGCAGTCgttcaggtt                       >  4:78103/1‑149 (MQ=255)
                                                                                                                     ggCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      >  7:245569/1‑100 (MQ=255)
                                                                                                                     ggCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                      <  8:245569/100‑1 (MQ=255)
                                                                                                                       ccTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                   >  8:4104/1‑149 (MQ=255)
                                                                                                                              tCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg             <  3:263591/148‑1 (MQ=255)
                                                                                                                               cGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        >  4:11324/1‑88 (MQ=255)
                                                                                                                               cGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                        <  3:11324/88‑1 (MQ=255)
                                                                                                                                gTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          <  3:44044/149‑1 (MQ=255)
                                                                                                                                gTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  7:139237/1‑149 (MQ=255)
                                                                                                                                 tGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg         >  6:233662/1‑149 (MQ=255)
                                                                                                                                  gATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCTACTCTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc        <  3:228569/149‑1 (MQ=255)
                                                                                                                                   aTAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc        <  3:195910/148‑1 (MQ=255)
                                                                                                                                        gCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  1:20360/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
TGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674605‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑