Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,769,864:1 +A 7.0% coding (1008/4716 nt) PP_5060 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,769,8641.A 7.0% 223.4 / 10.3 57coding (1008/4716 nt)PP_5060hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (31/22);  new base A (2/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCCGGCAGACCGTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGG  >  NC_002947/5769721‑5770011
                                                                                                                                                  |                                                                                                                                                   
tCCGGCAGACCGTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATg                                                                                                                                                >  2:268399/1‑149 (MQ=255)
           gTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACGGAACt                                                                                                                                     >  4:207288/1‑149 (MQ=255)
                   tGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGCGGGGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCg                                                                                                                                >  1:77389/1‑148 (MQ=255)
                      ccTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtgg                                                                                                                                     <  3:65600/138‑5 (MQ=255)
                      ccTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtgg                                                                                                                                     >  4:65600/1‑134 (MQ=255)
                       cTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtg                                                                                                                                      >  1:114986/1‑133 (MQ=255)
                       cTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTGACCATGG‑CACTggtg                                                                                                                                      <  2:114986/136‑4 (MQ=255)
                        tGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCAATGAACTCGGCGAAAAatga                                                                                                                        >  1:108806/1‑149 (MQ=255)
                         gcgcTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCCACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAatcat                                                                                                                       >  7:55966/1‑149 (MQ=255)
                          cgcTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAatgatg                                                                                                                      >  6:49662/1‑149 (MQ=255)
                             tGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCgg                                                                                                                   >  3:240464/1‑149 (MQ=255)
                                 ctgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTAGCGACAGGCCCTTGTGAAGCGCCAGGCCCAGACCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATTAATCGGGGTc                                                                                                               >  4:143158/1‑149 (MQ=255)
                                  tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACt                                                                                                                                          <  1:205315/121‑1 (MQ=255)
                                  tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACt                                                                                                                                          >  2:205315/1‑121 (MQ=255)
                                  tgtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCg                                                                                                              >  4:164423/1‑149 (MQ=255)
                                   gtgctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGc                                                                                                             >  3:41056/1‑149 (MQ=255)
                                     gctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAg                                                                                                           >  5:71057/1‑149 (MQ=255)
                                      ctgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAg                                                                                                           <  7:19612/148‑1 (MQ=255)
                                       tgtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGc                                                                                                         >  3:137008/1‑149 (MQ=255)
                                        gtTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGc                                                                                                         >  7:31184/1‑148 (MQ=255)
                                         ttGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGcc                                                                                                        <  6:71057/148‑1 (MQ=255)
                                                 gCGACAAGTCGATCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                               <  8:104706/149‑1 (MQ=255)
                                                  cGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                               >  2:189142/1‑148 (MQ=255)
                                                  cGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCag                                                                                               <  1:189142/148‑1 (MQ=255)
                                                   gACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CTATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCagta                                                                                             <  7:21144/149‑1 (MQ=255)
                                                    aCAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGaa                                                                                                                              >  4:101175/1‑115 (MQ=255)
                                                    aCAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGaa                                                                                                                              <  3:101175/115‑1 (MQ=255)
                                                      aaGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTg                                                                                          <  5:17400/149‑1 (MQ=255)
                                                         tCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTc                                                                                       >  7:56532/1‑149 (MQ=255)
                                                           gAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTcc                                                                                      <  5:49662/148‑1 (MQ=255)
                                                               aTGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                 <  7:165841/149‑1 (MQ=255)
                                                                    ttGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGa                                                                            >  7:32906/1‑149 (MQ=255)
                                                                         ggTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTg                                                                       >  1:102947/1‑149 (MQ=255)
                                                                          gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                 >  8:67721/1‑138 (MQ=255)
                                                                          gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTg                                                                                 <  7:67721/138‑1 (MQ=255)
                                                                          gTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGt                                                                      >  2:198351/1‑149 (MQ=255)
                                                                             aGC‑‑GGTCTGGCTATCCGTATCGGCTCGCCACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGa                                                                   >  1:152218/1‑149 (MQ=255)
                                                                               c‑‑GGTCTGGCTATCCGTATCGGGTCGGGACAGGCCCTTGGGAAGCGACAGGCCCAGCCCACCAGTG‑GCATGGTGACTGAAGTCTTCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATc                                                                 <  8:32906/149‑1 (MQ=255)
                                                                                          tATCCGTATCGGCTCGCGACAGACCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAg                                                        >  7:41520/1‑149 (MQ=255)
                                                                                            tCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAgc                                                       <  8:31184/148‑1 (MQ=255)
                                                                                              cGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAgcgc                                                     <  1:268399/148‑1 (MQ=255)
                                                                                               gTATCGGGTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAg                                                   >  3:215692/1‑149 (MQ=255)
                                                                                               gTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAg                                                   >  2:228579/1‑149 (MQ=255)
                                                                                                    ggCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCgg                                               <  8:41520/148‑1 (MQ=255)
                                                                                                              aGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTc                                    <  2:77389/149‑1 (MQ=255)
                                                                                                                  ccTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAg                                 >  6:62807/1‑148 (MQ=255)
                                                                                                                       ggAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCTACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGa                            <  1:198351/148‑1 (MQ=255)
                                                                                                                       ggAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGa                            <  5:62807/148‑1 (MQ=255)
                                                                                                                         aaGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGAttt                         >  7:27138/1‑149 (MQ=255)
                                                                                                                            cgcCAGGCCCAGCCCTCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCca                      <  4:240464/149‑1 (MQ=255)
                                                                                                                             gcCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCcag                     <  4:215692/149‑1 (MQ=255)
                                                                                                                              ccAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCcag                     >  8:49766/1‑148 (MQ=255)
                                                                                                                                    ccagcccaCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAg              >  7:112162/1‑149 (MQ=255)
                                                                                                                                    ccagcccaCCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAg              >  8:106543/1‑149 (MQ=255)
                                                                                                                                          caccaGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTg        <  3:164423/149‑1 (MQ=255)
                                                                                                                                           accaGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTg        >  6:132305/1‑148 (MQ=255)
                                                                                                                                                tG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAgg  <  3:207288/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCCGGCAGACCGTCCTGAATGCCCTGCGCTGGGCTGTGCTGTTGCAGTAGCGACAAGTCGAGCATGGCTTGGAGGTAAGC‑‑GGTCTGGCTATCCGTATCGGCTCGCGACAGGCCCTTGGGAAGCGCCAGGCCCAGCCCACCAGTG‑CCATGGTCACTGAACTCGGCGAAAAATGATGCGGGGTCGCAGGCCAAGTGGCAGTAGTGCTCCAGTTGGTCGACGCTGTCGATCGCACTCCAGCGCAGGCGGGCCACGCGCTCGAGCAAGATTTCCAGCAGCGAGGCGCTGTACAGG  >  NC_002947/5769721‑5770011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑