Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C97.7% 146.4 / ‑3.0 44intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (20/23);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCG  >  NC_002947/307719‑308001
                                                                                                                                                    |                                                                                                                                        
aGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑cc                                                                                                                                        >  7:73124/1‑148 (MQ=255)
   cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑ccgt                                                                                                                                      >  7:36072/1‑145 (MQ=255)
         ctcGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGa                                                                                                                                >  5:101747/1‑149 (MQ=255)
                cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTga                                                                                                                         <  5:118935/149‑1 (MQ=255)
                     cTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACTTGATCGGTgacgac                                                                                                                     >  3:144321/1‑148 (MQ=255)
                     cTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTgacgac                                                                                                                     >  5:90009/1‑148 (MQ=255)
                      ttCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACGTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGTCGAAA‑GCCGTTACCTGATCGGTGAcgacga                                                                                                                   <  1:86471/149‑1 (MQ=255)
                               ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                           <  8:73124/148‑1 (MQ=255)
                                 ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAg                                                                                                                  >  6:258420/1‑139 (MQ=255)
                                 ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAg                                                                                                                  <  5:258420/139‑1 (MQ=255)
                                                gCCTATGCACCGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCTAGACTACCCTTTCGGCTGACGAAATC‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                         >  4:227571/1‑149 (MQ=255)
                                                gCCTATGCACCGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt                                                                                         >  4:231310/1‑149 (MQ=255)
                                                 ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                           >  8:62177/1‑130 (MQ=255)
                                                 ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                        <  6:101747/149‑1 (MQ=255)
                                                    aTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAgg                                                                                                 <  8:155702/137‑1 (MQ=255)
                                                    aTGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAgg                                                                                                 >  7:155702/1‑137 (MQ=255)
                                                     tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                     >  7:71468/1‑148 (MQ=255)
                                                        aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                       >  3:155895/1‑127 (MQ=255)
                                                        aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                       <  4:155895/127‑1 (MQ=255)
                                                        aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                 <  6:90009/149‑1 (MQ=255)
                                                        aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑cc                                                                                                                                        >  1:148793/1‑92 (MQ=255)
                                                        aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑cc                                                                                                                                        <  2:148793/93‑2 (MQ=255)
                                                              cgGCCAACATCGGCGAAGAAGGCGATGTGACCCTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggc                                                                           <  7:183200/149‑1 (MQ=255)
                                                                     cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTTACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAg                                                                             <  4:130680/140‑1 (MQ=255)
                                                                     cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAg                                                                             >  3:130680/1‑140 (MQ=255)
                                                                            gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                             <  8:36072/149‑1 (MQ=255)
                                                                             aagaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg                                                            <  1:99886/149‑1 (MQ=255)
                                                                                aagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGc                                                         <  6:101484/149‑1 (MQ=255)
                                                                                          gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                               >  1:239278/1‑149 (MQ=255)
                                                                                            ccgtgtgCTTCCGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACTTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                             <  4:144321/146‑1 (MQ=255)
                                                                                             cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                  >  8:204718/1‑111 (MQ=255)
                                                                                             cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt                                                                                  <  7:204718/111‑1 (MQ=255)
                                                                                             cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                             >  1:47229/1‑148 (MQ=255)
                                                                                                 ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                        <  2:239278/149‑1 (MQ=255)
                                                                                                    ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                           >  2:48787/1‑127 (MQ=255)
                                                                                                    ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                           <  1:48787/127‑1 (MQ=255)
                                                                                                      cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                    <  3:48439/148‑1 (MQ=255)
                                                                                                        gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                    <  4:200178/146‑1 (MQ=255)
                                                                                                        gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                    >  3:200178/1‑146 (MQ=255)
                                                                                                        gCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAg                                 >  3:72349/1‑149 (MQ=255)
                                                                                                                           gACTACCCTTTCGGCTGACGAAA‑GCCGTTAACAGATCGGTGCCGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGGCCTGTCCGAGAAGAACGCGCCGGTCATCTGGAAAGCCa              >  1:11482/1‑149 (MQ=255)
                                                                                                                             cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                              <  8:154266/131‑1 (MQ=255)
                                                                                                                             cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                              >  7:154266/1‑131 (MQ=255)
                                                                                                                             cTACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc            <  2:47229/149‑1 (MQ=255)
                                                                                                                              tACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                             <  1:97656/99‑1 (MQ=255)
                                                                                                                              tACCCTTTCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                             >  2:97656/1‑99 (MQ=255)
                                                                                                                                     tCGGCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACg     <  4:72349/148‑1 (MQ=255)
                                                                                                                                       ggCTGACGAAA‑GCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg  >  7:219168/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
AGCCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAA‑G‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCG  >  NC_002947/307719‑308001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑