Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	100%	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	1 (0.020)	31 (0.760) +CGGG	28/250	NT	97.0%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	1 (0.020)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

TACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681278‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTG  >  NC_002947/5681416‑5681563                                                                                                                                            ||||                                                                                                                                                      TACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTG                                                                                                                                                >  7:101777/1‑149    ATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGAATGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTTTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCAATGCATGAACCGCAGGGGCTGCTGCG                                                                                                                                              >  1:60030/1‑149    ATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCGGCGC                                                                                                                                             >  8:143321/1‑149     TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTAGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCG                                                                                                                                              >  8:76753/1‑147        AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                                         >  7:115018/1‑149        AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                                         <  8:101777/149‑1              AGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCCGGCTTTAATTGCCACTGCATGAACCGCCCGGGCTGCAGCGCAGCCCTATCG                                                                                                                                   >  8:3166/1‑149               GACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCGGCGCAGCCCTATCGC                                                                                                                                  <  7:143321/149‑1                ACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGC                                                                                                                                  >  7:118171/1‑148                 CCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGA                                                                                                                                <  3:74720/149‑1                     CTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACAC                                                                                                                             <  8:118171/148‑1                              GCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACGCAAGGCCGCTC                                                                                                                   <  5:121855/149‑1                              GCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGGCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTC                                                                                                                   <  5:127846/149‑1                                   GTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCT                                                                                                                                                 >  2:189397/1‑114                                   GTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAACCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCT                                                                                                                                                 <  1:189397/114‑1                                             TGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGCCACAAGGCCGCTCCCACTCCAGACCACC                                                                                                    >  6:135002/1‑149                                                         GCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTACATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGC                                                                                                                                          <  4:199242/100‑1                                                         GCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTACATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGC                                                                                                                                          >  3:199242/1‑100                                                               ttgTTTGTGGGCGCGGCCTTGGGCCGCTAACGGGGCGCTAAGCGGCCCCAGGCTTTCATTCCCACTGCATGAACCGCCGGGGCGGCTGCGCAGCCCTATCGCCACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACA                                                                                   <  5:84271/146‑1                                                               TTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTC                                                                                                                   <  6:207156/117‑1                                                               TTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTC                                                                                                                   >  5:207156/1‑117                                                                              GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGGCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGCCCACCAGGCCCTGCGCCTCACATCCTAACCAAGGT                                                                      >  1:22040/1‑147                                                                                       cgctactGGGGCGCTAAGGGGCCCAAGGATTTCATTGCCACTGCATTACCCGCGGGGGCTGCTGCGCAGCCCTATCGCCACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGACAGGTTGCAGA                                                           <  8:115018/142‑1                                                                                          GAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTGCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCC                                                        >  3:167055/1‑149                                                                                             AGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCCCTCCAGCCCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCG                                                     >  5:277630/1‑149                                                                                                              CCAGGCTTTCTTTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACATGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTC                                   <  7:3166/149‑1                                                                                                                    TTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGC                             >  7:82493/1‑149                                                                                                                      TCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGC                             <  4:167055/147‑1                                                                                                                        ATTGCCACTGAATTAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACAAATGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGAC                          <  2:60030/148‑1                                                                                                                            CCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGACCAGGT                     <  2:22040/149‑1                                                                                                                                  CATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACCAGGCCGCTCCCACACCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCAATCCAATGCGACCAGGTCCGGT                >  5:196402/1‑148                                                                                                                                       ACCGCCGGGGCTGCGTCCCACCCCTAACGCGACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCG           <  7:76753/148‑1                                                                                                                                              GGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACCGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACC    <  1:192003/149‑1                                                                                                                                               GGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCGAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTG  <  6:196402/149‑1                                                                                                                                            ||||                                                                                                                                                      TACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATG‑CTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681278‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCA‑CGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTG  >  NC_002947/5681416‑5681563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.