Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	362,377	G→C	5.7%	L44V (CTG→GTG) ‡	PP_0301 ←	betainyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	362,377	0	G	C	5.7%	222.5 / 2.8	70	L44V (CTG→GTG) ‡	PP_0301	betainyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (33/33);  new base C (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ACACGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTAATCGC  >  NC_002947/362233‑362524                                                                                                                                                    |                                                                                                                                                    acacGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGcc                                                                                                                                                  >  3:216417/1‑148 (MQ=255)     gTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGc                                                                                                                                             >  4:233266/1‑149 (MQ=255)        aaGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGt                                                                                                                                          <  3:233266/149‑1 (MQ=255)             gCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGCAAAATGAGATTAATATGCCCAACCAGCTTAAAAACCAATTAACCGATTTCCCCCCGGATAAGGGGGACCAGCCCGATGCggac                                                                                                                                         >  1:218787/1‑146 (MQ=255)               ttGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCAAt                                                                                                                                      >  6:38856/1‑146 (MQ=255)                   gATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtag                                                                                                                                             <  8:240671/135‑3 (MQ=255)                   gATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtag                                                                                                                                             >  7:240671/1‑133 (MQ=255)                    aTCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCAt                                                                                                                              <  7:129697/149‑1 (MQ=255)                       aaaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtagc                                                                                                                                            <  1:197145/132‑4 (MQ=255)                       aaaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtagc                                                                                                                                            >  2:197145/1‑129 (MQ=255)                        aaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGt                                                                                                                          >  1:247325/1‑149 (MQ=255)                          gCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTgg                                                                                                                        >  3:212543/1‑149 (MQ=255)                             gAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCg                                                                                                                      >  3:126615/1‑148 (MQ=255)                             gAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGt                                                                                                                     <  5:15981/149‑1 (MQ=255)                               ggATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTa                                                                                                                    <  4:33287/148‑1 (MQ=255)                                gATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGc                                                                                                                  <  4:260963/149‑1 (MQ=255)                                            cACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcag                                                                                                        <  4:98544/147‑1 (MQ=255)                                            cACCCACACCTCCGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTCAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcag                                                                                                        <  4:94756/147‑1 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                                  <  1:7271/118‑1 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                                  >  2:7271/1‑118 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcagc                                                                                                       <  4:247225/145‑1 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcagc                                                                                                       >  3:247225/1‑145 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAgg                                                                                                    >  6:7874/1‑148 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGCTGGCGTAGCTGAAGATCAGCAgg                                                                                                    >  2:20665/1‑148 (MQ=255)                                               ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATAGGTTGCGTAGCTGAAGATCAGCAgg                                                                                                    >  4:169572/1‑148 (MQ=255)                                                    ccTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                              >  1:81873/1‑149 (MQ=255)                                                     cTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCgg                                                                                                                                           >  6:244088/1‑103 (MQ=255)                                                     cTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAgg                                                                                             >  5:189061/1‑149 (MQ=255)                                                        ggTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCACCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCa                                                                                           <  5:232293/148‑1 (MQ=255)                                                            cccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCACCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATcgcg                                                                                      >  2:21854/1‑149 (MQ=255)                                                             ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGTCCGATGCCGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATagcg                                                                                      >  2:50742/1‑148 (MQ=255)                                                             ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTa                                                                                                  >  1:253827/1‑136 (MQ=255)                                                             ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTa                                                                                                  <  2:253827/136‑1 (MQ=255)                                                               aGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAg                                                                                   <  4:212543/149‑1 (MQ=255)                                                                 cTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGt                                                                                 <  2:24964/149‑1 (MQ=255)                                                                        tCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTATGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGtt                                                                          >  1:202182/1‑149 (MQ=255)                                                                           tGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGt                                                                        >  6:4929/1‑148 (MQ=255)                                                                            gC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGa                                                                                                               <  3:48658/108‑1 (MQ=255)                                                                            gC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGa                                                                                                               >  4:48658/1‑108 (MQ=255)                                                                             c‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTaa                                                                      <  4:126615/148‑1 (MQ=255)                                                                               aaaT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATc                                                                    <  6:151740/149‑1 (MQ=255)                                                                               aaaT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATc                                                                    <  6:189061/149‑1 (MQ=255)                                                                                  t‑a‑atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                              <  3:215506/120‑1 (MQ=255)                                                                                  t‑a‑atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                              >  4:215506/1‑120 (MQ=255)                                                                                      atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                                  >  7:189209/1‑82 (MQ=255)                                                                                      atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                                  <  8:189209/82‑1 (MQ=255)                                                                                      atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGAcc                                                                <  2:218787/148‑1 (MQ=255)                                                                                                       gCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCgtg                                              <  4:216417/149‑1 (MQ=255)                                                                                                        cGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCgtgg                                             >  2:110743/1‑149 (MQ=255)                                                                                                           gAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGc                                          >  6:147045/1‑149 (MQ=255)                                                                                                                    gTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGa                                 >  3:4977/1‑149 (MQ=255)                                                                                                                     ttACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGGGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGAt                                <  3:169572/149‑1 (MQ=255)                                                                                                                             ttGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCACGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                        <  2:247325/149‑1 (MQ=255)                                                                                                                              tGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGTATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                        <  2:81873/148‑1 (MQ=255)                                                                                                                              tGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                        <  5:147045/148‑1 (MQ=255)                                                                                                                                ccggcgGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGTAGATCAGCAGGTTGAAGGGATCGCGCAGGTGGCCGTTGTAATCGTCGCTGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCa                     <  1:50742/146‑1 (MQ=255)                                                                                                                                cccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCa                     >  5:215577/1‑149 (MQ=255)                                                                                                                                 ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCgg                                        <  1:226182/129‑1 (MQ=255)                                                                                                                                 ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCgg                                        >  2:226182/1‑129 (MQ=255)                                                                                                                                 ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCAt                    >  1:267342/1‑149 (MQ=255)                                                                                                                                    cgGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCtt                 <  5:7874/149‑1 (MQ=255)                                                                                                                                     tgCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATATGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCGTTTGTAATCTACCCAGTCCTCCTGGACCGTGGTTCGGTAGGTGATCAGAGCGGGCATCTTg                <  1:20665/148‑1 (MQ=255)                                                                                                                                        tATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGtc             >  3:133549/1‑149 (MQ=255)                                                                                                                                           cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTg                <  6:205705/143‑1 (MQ=255)                                                                                                                                           cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTg                >  5:205705/1‑143 (MQ=255)                                                                                                                                           cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc          >  1:31587/1‑149 (MQ=255)                                                                                                                                            ggCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc          <  5:4929/148‑1 (MQ=255)                                                                                                                                            ggCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc          <  1:110743/148‑1 (MQ=255)                                                                                                                                               gTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTaa      <  2:202182/149‑1 (MQ=255)                                                                                                                                                   aGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTAATCGc  >  8:171925/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACACGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTAATCGC  >  NC_002947/362233‑362524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑