| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,125,096 | A→T | 13.8% | intergenic (+70/‑112) | PP_4511 → / → PP_4512 | AraC family transcriptional regulator/DMT superfamily permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,125,096 | 0 | A | T | 13.8% | 76.2 / 4.5 | 36 | intergenic (+70/‑112) | PP_4511/PP_4512 | AraC family transcriptional regulator/DMT superfamily permease |
| Reads supporting (aligned to +/- strand): ref base A (15/16); new base T (3/2); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGCCTTTTCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAACCACCGCACCGCCCTCGGCGCACTGCA > NC_002947/5124949‑5125239 | aGTGCCTTTTCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTa > 5:25733/1‑148 (MQ=255) ccTTTTCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAAcc < 6:25733/148‑1 (MQ=255) ggtctggtggccagtttggcgaggacgccggtaaggccgaagaacagcgcgccaatgtgcagtgcgccgagggcggtgcggttgttcatggtgggtccttgatggagatctctacagtcagtgccggccctTTCGCGGGTAAACCCGc > 8:234203‑M1/132‑148 (MQ=255) ttCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGc < 8:115782/147‑1 (MQ=255) tCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTc < 5:210331/148‑1 (MQ=255) tCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTc > 3:229017/1‑148 (MQ=255) cAGCGGCGATTCTGCGGGCGTTCGGGAGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTTTACCCGcgaa < 6:227402/148‑4 (MQ=255) aGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGTCGGCCCCTTCGCGGGTTTACCCGcgaag < 7:167009/148‑5 (MQ=255) aGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTa > 4:51606/1‑149 (MQ=255) gcgATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAgg < 3:51606/149‑1 (MQ=255) tgcGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTTAAAACCCGCCAAAAGGGTGcccc > 3:44843/1‑149 (MQ=255) gcGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGccccc > 1:125179/1‑149 (MQ=255) gCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGGCCCTTCGCGGGTAAAACCCCTCCAACACGATGCCCCCGc > 5:233342/1‑147 (MQ=255) gCGTTCGGGGGGACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGGCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCt < 4:195824/149‑1 (MQ=255) gTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTTAAACCCCACCAAAACGATGGCccccccgg > 3:127036/1‑146 (MQ=255) cGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACa > 3:76876/1‑130 (MQ=255) cGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACa < 4:76876/130‑1 (MQ=255) ggTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAAtgtt < 2:67511/149‑1 (MQ=255) ccTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCACCTACAGACTGCCCCCGCCAGAAAGTTGTGCaaaa > 2:81442/1‑149 (MQ=255) cgaagaacagcgcgccaatgtgcagtgcgccgagggcggtgcggtggttcatggtgggtccttgatggagatctctacaggcagtgccggccctTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCGGAATGTTGTGCAAAAcc < 1:41662‑M1/55‑1 (MQ=255) tGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGGCTGAATGTTGTGGaaaaacgg > 6:216990/1‑145 (MQ=255) tttGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAAACCCCTGCAACAAGCTGCCCCCCCCGGAAAGTGGTGCAAAAccggg > 2:224930/1‑143 (MQ=255) cggcgAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCg < 2:125179/149‑1 (MQ=255) ggcgAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGTCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCgg < 6:164597/149‑1 (MQ=255) ggCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTTAACACGCACCTACAGGATGCCGCCGCCGGTAAGTTGTGCAAAAACGGTAGGTGCCGGTTATCCcgg > 7:184726/1‑147 (MQ=255) ccgagggcggtgcggtggttcatggtgggtccttgatggagatctctacaggcagtgccggccctTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCGGGTTTACCCGCGAAAGGGc > 2:41662‑M1/66‑149 (MQ=255) aCAGCTGTGTTGCTACATATTGTGTTGGCCTGTGGTTTTACACTTTGCCTTTGCCGGCCCGTTCGCGGGAAAACCCGCTCCTACTGGATGCCCCCGCCTTAATGTTTTGAAAACCCTTTTGGCGGGGTTTTACCCGCGAAAGGGCCgg < 6:233342/148‑1 (MQ=255) cagTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGc > 8:120911/1‑121 (MQ=255) cagTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGc < 7:120911/121‑1 (MQ=255) tggtgggtccttgatggagatctctacaggcagtgccggccctTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGt < 7:131664‑M1/58‑1 (MQ=255) tggtgggtccttgatggagatctctacaggcagtgccggccctTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGt > 8:131664‑M1/44‑101 (MQ=255) gCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGCATGTTGTGCAAAACCTGTTGGAGCGGGTTTACCCGCGGAAGGGCCGGCCCTGCCTGTAGAGATGTCCATCAAGGa > 1:39007/1‑149 (MQ=38) acTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCCCTCCAACAAGAAGCCCCCCCCGGAATTTTGTGCAAAAACTGTAGGATCGGGTTTAACCGCGAAATGGCCCGCACAGCCAGTATAGAAATCCATAAACGACCCACCAAAAaccc > 6:250742/1‑148 (MQ=255) atttgcctttgccGGGCCCTTCGCTGGTAAACGCGCTCCTACTGGATTCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAaccac < 8:184726/148‑1 (MQ=38) cctttgccGGCCCCTTCGCGGTTAACCCCGCTCCTACAGGATCCCCCCGCCTTATTGTTGTGCAAAACCTTTAGGAGGGGTTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAACcaccgca < 8:24222/148‑1 (MQ=38) CGGGTAACCCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAACCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAACCACCGCACCGCCCTCGGCGCACTGca > 2:39007‑M1/1‑149 (MQ=255) CGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAACCACCGCACCGCCCTCGGCGCACTGca < 4:186093‑M1/149‑1 (MQ=255) | AGTGCCTTTTCAGCGGCGATGCTGCGGGCGTTCGGGTGTACACCTTTGGCTTTGCGGCGAGAGTCTGGCGATAACTGACAGCAGTCTTGCGACAGAATGTGTTTGCCTGAGGCTTTACACTTTGCCTTTGCCGGCCCCTTCGCGGGTAAACCCGCTCCTACAGGATGCCCCCGCCTGAATGTTGTGCAAAACCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGCCTGTAGAGATCTCCATCAAGGACCCACCATGAACCACCGCACCGCCCTCGGCGCACTGCA > NC_002947/5124949‑5125239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |