Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,168,313 G→C 7.8% intergenic (‑434/‑70) hrpA ← / → PP_4547 ATP‑dependent helicase HrpA/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,168,3130GC 7.8% 147.0 / 2.6 50intergenic (‑434/‑70)hrpA/PP_4547ATP‑dependent helicase HrpA/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base C (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTGTTC  >  NC_002947/5168183‑5168457
                                                                                                                                  |                                                                                                                                                 
gAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCaa                                                                                                                                 >  1:183028/1‑149 (MQ=255)
      ccGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGgc                                                                                                                           >  8:177711/1‑149 (MQ=255)
       cGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCTCCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCggg                                                                                                                            <  2:1984/147‑1 (MQ=255)
       cGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGgca                                                                                                                          >  1:1984/1‑148 (MQ=255)
         gAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGgccgc                                                                                                                        >  6:8007/1‑149 (MQ=255)
         gAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGgccgc                                                                                                                        <  1:230310/149‑1 (MQ=255)
                  cGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTgcg                                                                                                               >  6:253536/1‑149 (MQ=255)
                      gAAAATCCGCTGGTCCACATAATCCTCGCCTTCTGGTCCTTGT‑GAAATGCCTTCACGCGCGACAAACGGACACTTCGCTAACGCAATTGTGACCAAACCAGGAAAGCGTCTGGCCGTTTGACTAAACCGTGCAGCATGCATGCGCGGTg                                                                                                          >  4:86396/1‑149 (MQ=255)
                        aaaTCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTgg                                                                                                         >  4:139979/1‑149 (MQ=255)
                        aaaTCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTgg                                                                                                         <  5:214581/149‑1 (MQ=255)
                           tCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACACCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGa                                                                                                                                      >  1:122621/1‑117 (MQ=255)
                           tCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACACCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGa                                                                                                                                      <  2:122621/117‑1 (MQ=255)
                              gCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTgc                                                                                                   >  2:212884/1‑149 (MQ=255)
                                     cacaTCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCt                                                                                            <  5:253536/148‑1 (MQ=255)
                                       catcatCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTgg                                                                                                                                              >  4:98092/1‑97 (MQ=255)
                                       catcatCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTgg                                                                                                                                              <  3:98092/97‑1 (MQ=255)
                                        atcatcCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTgcgc                                                                                                              >  3:186342/1‑128 (MQ=255)
                                        atcatcCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTgcgc                                                                                                              <  4:186342/128‑1 (MQ=255)
                                                           ccTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACtt                                                                     >  5:90010/1‑149 (MQ=255)
                                                           ccTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACtt                                                                     >  5:34097/1‑149 (MQ=255)
                                                              tgtgGCACTGCCGTCACGCGCGCCCACCGGACTCTTCGCTCACGCCATTGTGACCAAACCAGGACAGCGTCTGGCCGTTTGACTCAACAGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTGTATGAGCGAATTGATCATGCACTTTGc                                                                  >  7:202447/1‑149 (MQ=255)
                                                                  gCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAAtt                                                                                 >  8:95139/1‑130 (MQ=255)
                                                                  gCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAAtt                                                                                 <  7:95139/130‑1 (MQ=255)
                                                                         cGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTagag                                                        >  1:117038/1‑148 (MQ=255)
                                                                            cACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATg                                                                          >  4:74192/1‑127 (MQ=255)
                                                                            cACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATg                                                                          <  3:74192/127‑1 (MQ=255)
                                                                                  cgcCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTAccc                                              <  7:177711/149‑1 (MQ=255)
                                                                                      cACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCg                                                                                     <  2:155128/106‑1 (MQ=255)
                                                                                      cACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCg                                                                                     >  1:155128/1‑106 (MQ=255)
                                                                                          ggACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGCACCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCt                                       <  1:212884/149‑1 (MQ=255)
                                                                                                   gCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATc                                                                             <  5:14069/101‑1 (MQ=255)
                                                                                                   gCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATc                                                                             >  6:14069/1‑101 (MQ=255)
                                                                                                       aCGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAAcccc                          >  3:221530/1‑148 (MQ=255)
                                                                                                       aCGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAAccc                           <  6:90010/147‑1 (MQ=255)
                                                                                                             tcgtgACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCg                   <  2:170296/147‑1 (MQ=255)
                                                                                                                tgACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCTGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAg                 <  3:86396/148‑1 (MQ=255)
                                                                                                                 gACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGCATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCt               >  6:167776/1‑149 (MQ=255)
                                                                                                                 gACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGc                                    <  1:146558/128‑1 (MQ=255)
                                                                                                                 gACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGc                                    >  2:146558/1‑128 (MQ=255)
                                                                                                                     aaaCCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTc           <  2:117038/149‑1 (MQ=255)
                                                                                                                     aaaCCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTc           >  2:122058/1‑149 (MQ=255)
                                                                                                                       aaccGGCCAGACGCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGat                       <  6:180454/133‑1 (MQ=255)
                                                                                                                       aaacGGCCAGACGCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGat                       >  5:180454/4‑135 (MQ=255)
                                                                                                                        aacGGCCAGACGCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGaa                                                                                   >  8:252792/3‑74 (MQ=255)
                                                                                                                        aacGGCCAGACGCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGaa                                                                                   <  7:252792/72‑1 (MQ=255)
                                                                                                                         ccGGCCAGCGTCTGGCCGTTTGACTCAACAGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAAc       >  2:220868/1‑149 (MQ=255)
                                                                                                                           ggCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTg     <  5:8007/149‑1 (MQ=255)
                                                                                                                              cAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTGtt   >  2:192619/1‑148 (MQ=255)
                                                                                                                              cAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTGTTc  <  6:34097/149‑1 (MQ=255)
                                                                                                                              cAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑CCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTGTTc  <  1:220868/149‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                 
GAAGGCCCGGAGCCGTGCCGGGGAAAATCCGCTGGTCCACATCATCCTCGCCTTCTGGGCCTTGTGGCACTGCCGTCACGCGCGCCCACCGGACACTTCGCTCACGCCATTGTGACCAAACCCGGCCAGCGTCTGGCCGTTTGACTCAACCGGGCCGCCTGCTTGCGCGGTGGCCCTGC‑GCGTCTATGAGCGAATTGATCATGCACTTTGCCCCTGTAGAGCAGGCTACCCGTTTTCTGGCCGACAACCCCGATATCGAGCTGTTCGAACTGTTC  >  NC_002947/5168183‑5168457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑