Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,212,488 C→A 10.0% intergenic (+52/+42) PP_4593 → / ← PP_4594 hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,212,4880CA10.0% 148.2 / 6.9 50intergenic (+52/+42)PP_4593/PP_4594hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base A (3/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

CCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCC  >  NC_002947/5212360‑5212631
                                                                                                                                 |                                                                                                                                               
ccGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAc                                                                                                                             >  5:131358/1‑149 (MQ=255)
   tCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGTCAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcg                                                                                                                          <  4:28774/149‑1 (MQ=255)
    cAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCcgc                                                                                                                         >  6:32122/1‑149 (MQ=255)
        cGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGaaa                                                                                                                     <  5:61261/149‑1 (MQ=255)
                cAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCAACCGGCCTATTCGCGGGCAAGCCCGCTCCTACCCCCTCCTATGAGCGGCTTTACCCGCGAAAGGGCCtcc                                                                                                             >  4:166604/1‑147 (MQ=255)
                 aaGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAACCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                 <  7:28428/128‑1 (MQ=255)
                 aaGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAAACCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGt                                                                                                                                 >  8:28428/1‑128 (MQ=255)
                   ggAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGACGGTGTAGGAGCgg                                                                                                                                   >  4:2505/1‑124 (MQ=255)
                   ggAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGACGGTGTAGGAGCgg                                                                                                                                   <  3:2505/124‑1 (MQ=255)
                   ggAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                   >  5:269935/1‑140 (MQ=255)
                   ggAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAgg                                                                                                                   <  6:269935/140‑1 (MQ=255)
                                 tCAAGGACAAGATCGAACGGATCATTCATCCGGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGa                                                                                             >  1:168809/1‑149 (MQ=255)
                                  cAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAcc                                                                                           >  7:90783/1‑149 (MQ=255)
                                        cAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAgcgc                                                                                      <  8:238603/148‑1 (MQ=255)
                                         aaGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCtt                                                                                    >  8:202834/1‑149 (MQ=255)
                                              cGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAAc                                                                               >  4:141594/1‑149 (MQ=255)
                                                aaCGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGc                                                                             <  2:168809/149‑1 (MQ=255)
                                                  cGGATCATTCATGC‑GCCCATATCCTGTCAGTCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCTAGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACggc                                                                            <  2:128707/148‑3 (MQ=255)
                                                   ggATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                          <  4:19192/149‑1 (MQ=255)
                                                   ggATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                          <  6:131358/149‑1 (MQ=255)
                                                    gATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTTCCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                                         >  1:220895/1‑149 (MQ=255)
                                                    gATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCa                                                                         <  3:141594/149‑1 (MQ=255)
                                                     aTCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCAt                                                                        >  3:145770/1‑149 (MQ=255)
                                                        attcatCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATc                                                                       >  6:201126/1‑147 (MQ=255)
                                                         ttcatCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCATACAGCGACCAGCGCTTTCAACGCCCCATCCAg                                                                    <  2:198043/149‑1 (MQ=255)
                                                         ttcatCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAg                                                                    <  8:90783/149‑1 (MQ=255)
                                                            atCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTc                                                                 >  1:132921/1‑149 (MQ=255)
                                                               c‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              <  5:201126/149‑1 (MQ=255)
                                                               c‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              <  5:32122/149‑1 (MQ=255)
                                                                 gCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              >  3:143773/1‑148 (MQ=255)
                                                                 gCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCg                                                             <  4:145770/149‑1 (MQ=255)
                                                                 gCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCg                                                             <  7:202834/149‑1 (MQ=255)
                                                                     aaaTCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                                           >  7:173314/1‑147 (MQ=255)
                                                                     aaaTCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTAACCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGAc                                                           >  7:173313/1‑147 (MQ=255)
                                                                                 gCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                         >  7:20224/1‑137 (MQ=255)
                                                                                 gCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                         <  8:20224/137‑1 (MQ=255)
                                                                                    tCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTGAACGcccc                                                                          >  7:5192/1‑117 (MQ=255)
                                                                                    tCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcccc                                                                          <  8:5192/117‑1 (MQ=255)
                                                                                    tCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGTTCCTCGACCAGGTCACgg                                                 <  8:221798/142‑1 (MQ=255)
                                                                                    tCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACgg                                                 >  7:221798/1‑142 (MQ=255)
                                                                                        ttGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGAt                                      <  2:220895/149‑1 (MQ=255)
                                                                                                    gCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACaa                          >  2:265672/1‑149 (MQ=255)
                                                                                                            gcgGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcagca                  <  1:265672/149‑1 (MQ=255)
                                                                                                                     cccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGc         >  3:186885/1‑149 (MQ=255)
                                                                                                                      ccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                         <  3:258633/100‑1 (MQ=255)
                                                                                                                      ccGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                                         >  4:258633/1‑100 (MQ=255)
                                                                                                                       cGCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCt        >  7:44298/1‑148 (MQ=39)
                                                                                                                        gCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                 >  7:134841/1‑138 (MQ=39)
                                                                                                                        gCTCCTACGACGGTGTAGGAGCGGGCTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGcagcag                 <  8:134841/138‑1 (MQ=39)
                                                                                                                             tACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATccc  >  1:252984/1‑148 (MQ=255)
                                                                                                                              acacCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              >  7:66116/1‑87 (MQ=255)
                                                                                                                              acacCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTc                                                              <  8:66116/87‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                               
CCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCC‑GCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTCTCGCTGATCCC  >  NC_002947/5212360‑5212631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑