Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	372,628	T→C	12.3%	intergenic (+48/‑131)	dgcA → / → dgcB	dimethylglycine dehydrogenase subunit/dimethylglycine dehydrogenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	372,628	0	T	C	12.3%	100.6 / 5.9	41	intergenic (+48/‑131)	dgcA/dgcB	dimethylglycine dehydrogenase subunit/dimethylglycine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base T (15/20);  new base C (2/3);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

GCGAAGGCTACCTGCTGTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAAC  >  NC_002947/372483‑372767                                                                                                                                                  |                                                                                                                                            gCGAAGGCTACCTGCTGTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTAcc                                                                                                                                          <  3:205104/149‑1 (MQ=255)        cTACCTGCTGTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAAt                                                                                                                                   <  8:206603/149‑1 (MQ=255)           ccTGCTGTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTAAACCcgctcctaca                                                                                                                                <  4:208707/149‑8 (MQ=255)               ctgTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAg                                                                                                                            >  7:221970/1‑149 (MQ=255)                    ccGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACt                                                                                                                        >  8:179749/1‑148 (MQ=255)                           ggCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGacaaca                                                                                                                 <  1:137172/148‑1 (MQ=255)                           ggCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGacaaca                                                                                                                 <  6:233908/148‑1 (MQ=255)                            gCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACt                                                                                                               >  2:96346/1‑149 (MQ=255)                                        cccAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTCACCcgc                                                                                                                                       <  8:98641/113‑1 (MQ=255)                                        cccAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTCACCcgc                                                                                                                                       >  7:98641/1‑113 (MQ=255)                                         ccAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCg                                                                                                   >  2:167182/1‑148 (MQ=255)                                         ccAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCg                                                                                                   >  5:76903/1‑148 (MQ=255)                                           aGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGAc                                                                                                <  7:179749/149‑1 (MQ=255)                                                aaCGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAAGAGCGGGTTCACCCGCGAATGCGTCCGGTCAGGCAACAGTGTTGTCAGTGCTGACGCAtt                                                                                           <  1:110699/149‑1 (MQ=18)                                                  cGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTcg                                                                                         >  7:113695/1‑149 (MQ=255)                                                   gTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAAGAGCGGGTTCACCCGCGAATGCGTCCGGTCAGGCAACAGTGTTGTCAGTGCTGACGCATTcgc                                                                                        <  6:182367/149‑1 (MQ=18)                                                      cATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCgg                                                                                      <  6:242169/148‑1 (MQ=255)                                                      cATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCgg                                                                                      <  8:39926/148‑1 (MQ=255)                                                            gcgATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTc                                                                                                                              <  4:9850/102‑1 (MQ=255)                                                            gcgATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTc                                                                                                                              >  3:9850/1‑102 (MQ=255)                                                                    cgacgCCTTGCGCCTGTGCAAGGACTTCTGATCGTTTACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGTGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCGGACCGGACGCATTCGCGGGTGAACCCGc                                                                            <  5:232406/144‑1 (MQ=255)                                                                    cgacgCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGctc                                                                          <  8:221970/146‑1 (MQ=255)                                                                           ttGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATgggg                                                                >  2:126508/1‑149 (MQ=255)                                                                                   gtgCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGtctc                                                        <  3:36853/149‑1 (MQ=255)                                                                                    tgCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGtctc                                                        <  6:76903/148‑1 (MQ=255)                                                                                           aCTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTg                                                >  4:69405/1‑149 (MQ=255)                                                                                             ttCTGATCGTTAACAGTCCCCGCCATCGGGCGCGATCCCTCTAGCAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCCCGGGTGAACCCGCTCTTACAAGGGCCCAGTCTCGCCCTCTGGt                                              >  2:118658/1‑149 (MQ=255)                                                                                                  aTCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGgcg                                         >  6:230665/1‑149 (MQ=255)                                                                                                      ttAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGgcgc                                        >  8:112191/1‑146 (MQ=255)                                                                                                             tCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGc                                                                                                                                 <  3:213219/50‑1 (MQ=255)                                                                                                             tCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGc                                                                                                                                 >  4:213219/1‑50 (MQ=255)                                                                                                                   ccATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGttt                        <  8:113695/149‑1 (MQ=255)                                                                                                                                   cTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATg        <  3:69405/149‑1 (MQ=255)                                                                                                                                      tAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTg     <  1:167182/149‑1 (MQ=255)                                                                                                                                      tAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTg     <  5:262598/149‑1 (MQ=255)                                                                                                                                      cccccacGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGtt      <  1:96346/142‑1 (MQ=255)                                                                                                                                       aGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCggg                                                                                     >  6:35329/1‑68 (MQ=255)                                                                                                                                       aGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCggg                                                                                     <  5:35329/68‑1 (MQ=255)                                                                                                                                         gAGCGGGTTCACCCGCGAATGCGGGCGGTCAGGCAACAGTGTTGTCAGTGCTGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAac  >  2:75457/1‑149 (MQ=18)                                                                                                                                          aGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTccc           <  7:33851/139‑1 (MQ=255)                                                                                                                                          aGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTccc           >  8:33851/1‑139 (MQ=255)                                                                                                                                                  |                                                                                                                                            GCGAAGGCTACCTGCTGTACCGCATCGGCGACTGCGTGGCCCAGCGCAACGTGCATGCGGCGATCTACGACGCCTTGCGCCTGTGCAAGGACTTCTGATCGTTAACAGTCGCCGCCATCGGGCGCGATCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCAGCACTGACAACACTGTTGCCTGACCGGACGCATTCGCGGGTGAACCCGCTCTTACATGGGGCCAGTCTCGCCCTCTGGTCGGCGCAAGAATCCAGCTGTTTGTGGGAGCCTCCCATGTTGAAC  >  NC_002947/372483‑372767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑