Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,222,188 C→A 8.7% E134D (GAG→GAT) ‡ PP_4602 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,222,1880CA 8.7% 142.3 / 4.4 46E134D (GAG→GAT) ‡PP_4602AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (18/24);  new base A (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

ACTGCGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCGGTTG  >  NC_002947/5222057‑5222328
                                                                                                                                   |                                                                                                                                             
aCTGCGGCCAGGAGATGGTAGGGGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATTCCGACCAGGCCGATGATTTCGCCTGCAGGGTTGTAGAGCGGGCGATTGTGGGTCAGGCACCTGCCGGGCTCACGGCTGCCGTagag                                                                                                                              <  5:202588/149‑1 (MQ=255)
   gCGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGTGAGCCCGGCTgg                                                                                                                                     >  6:61861/1‑138 (MQ=255)
   gCGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGTGAGCCCGGCTgg                                                                                                                                     <  5:61861/139‑2 (MQ=255)
    cGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGCTGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGc                                                                                                                          <  3:175737/149‑1 (MQ=255)
    cGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGt                                                                                                                            <  2:21001/147‑1 (MQ=255)
      gCCTGGCGCTGGTAGGCGTGGTGGCTGTCAGGGGCTGACTGCAAGTCGACCGAGATGCCGTGCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCTGGCGGTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAg                                                                                                                        <  2:99115/149‑1 (MQ=255)
         aGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTc                                                                                                                     >  4:85484/1‑149 (MQ=255)
             gcTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAAc                                                                                                                 <  1:222383/149‑1 (MQ=255)
                     gCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGtcctc                                                                                                         >  2:235155/1‑149 (MQ=255)
                      cGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGCTGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGtcctc                                                                                                         >  2:187917/1‑148 (MQ=255)
                      cGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTgg                                                                                                              <  1:125286/143‑1 (MQ=255)
                      cGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTgg                                                                                                              >  2:125286/1‑143 (MQ=255)
                         ggTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGc                                                                                                     <  3:41386/149‑1 (MQ=255)
                         ggTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGc                                                                                                     <  4:164265/149‑1 (MQ=255)
                          gTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCa                                                                                                    >  3:104786/1‑149 (MQ=255)
                                 tCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGc                                                                                              <  3:85484/148‑1 (MQ=255)
                                      ggCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGccttcct                                                                                        >  3:214757/1‑149 (MQ=255)
                                           aCTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTgag                                                                                    <  2:138347/148‑1 (MQ=255)
                                           aCTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTga                                                                                     <  8:155221/147‑1 (MQ=255)
                                               cAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGccttcctt                                                                                       <  7:136030/141‑1 (MQ=255)
                                               cAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGccttcctt                                                                                       >  8:136030/1‑141 (MQ=255)
                                                 aGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGt                                                                             <  4:214757/149‑1 (MQ=255)
                                                 aGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGt                                                                             <  3:258390/149‑1 (MQ=255)
                                                   tCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCg                                                                           <  8:87510/149‑1 (MQ=255)
                                                        cGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGc                                                                                              <  4:188840/125‑1 (MQ=255)
                                                        cGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGc                                                                                              >  3:188840/1‑125 (MQ=255)
                                                            aTGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGTGAGCCCGGCTg                                                                                                                                      <  1:235415/81‑1 (MQ=255)
                                                            aTGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGTGAGCCCGGCTg                                                                                                                                      >  2:235415/1‑81 (MQ=255)
                                                                       gCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCgg                                                      >  1:34485/1‑149 (MQ=255)
                                                                         cGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCgggg                                                    <  1:225523/149‑1 (MQ=255)
                                                                             gatTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCa                                                 >  5:27803/1‑148 (MQ=255)
                                                                             gatTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCa                                                 >  8:181650/1‑148 (MQ=255)
                                                                              atTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCCGTATAGCCGGGGCCaaa                                               >  7:43976/1‑149 (MQ=255)
                                                                                             ttGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAacac                                <  1:235155/149‑1 (MQ=255)
                                                                                                  cAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACAGGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCgg                           <  7:181650/149‑1 (MQ=255)
                                                                                                     cGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGccttcctt                                                                                       >  2:90732/1‑87 (MQ=255)
                                                                                                     cGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGccttcctt                                                                                       <  1:90732/87‑1 (MQ=255)
                                                                                                     cGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTGCCTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGgcg                         <  1:187917/149‑1 (MQ=255)
                                                                                                     cGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGgcgc                        <  6:27803/149‑1 (MQ=255)
                                                                                                            ttGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCCCCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCtt                 >  8:226462/1‑149 (MQ=255)
                                                                                                              gtgGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTg                <  3:45538/148‑1 (MQ=255)
                                                                                                                  gTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCcag           >  7:164017/1‑149 (MQ=255)
                                                                                                                         aCCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCATCTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCGGt    >  8:85526/1‑149 (MQ=255)
                                                                                                                             gCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCg      >  7:11525/1‑143 (MQ=255)
                                                                                                                             gCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCg      <  8:11525/143‑1 (MQ=255)
                                                                                                                             gCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACATCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCGGTTg  >  6:177568/1‑147 (MQ=255)
                                                                                                                                   |                                                                                                                                             
ACTGCGGCCAGGCGCTGGTAGGCGGGGTGGCTGTCAGCGGCTGACTGCAAGTCGACCGAGATGCCGACCAGGCCGATGATTTCGCCGGCAGGGTTGTACAGCGGGCGCTTGTGGGTCAGGCACCAGCCGGGCTCACGGCTGCCGTAGAGGTGCAGCTCCAACTGGTCCTCCAGCACCAGGCCTTCCTTGAGAACACGTCGATCCTG‑CTCGGTATAGCCGGGGCCAAACTGTGCCGGAAACACTTCGGCGCTGGTCTTGCCCAGCAGCGGTTG  >  NC_002947/5222057‑5222328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑