Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 375,629 G→A 7.8% A307T (GCA→ACA)  PP_0312 → electron transfer flavoprotein subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947375,6290GA 7.8% 145.9 / 3.4 51A307T (GCA→ACA) PP_0312electron transfer flavoprotein subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TCAACAAGCGTGGTGCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATC  >  NC_002947/375482‑375766
                                                                                                                                                   |                                                                                                                                         
tCAACAAGCGTGGTGCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCg                                                                                                                                           >  6:272714/1‑148 (MQ=255)
  aacaaGCGTGGTGCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGCCTGGGACCTGTACCACAAGGCCACCGCAg                                                                                                                                        >  3:52735/1‑149 (MQ=255)
         gtggtgCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCgg                                                                                                                                 <  3:154147/149‑1 (MQ=255)
         gtggggCGTCGCCGGCCGCGCCTCGAAGTCCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGGCTCGGGCGGCAACGGGGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCgg                                                                                                                                 <  8:254473/149‑1 (MQ=255)
          tggtgCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGgc                                                                                                                                >  3:76976/1‑149 (MQ=255)
               cgcAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTAc                                                                                                                           <  4:38700/149‑1 (MQ=255)
                     aggctgcgcATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAgg                                                                                                                     >  5:233139/6‑149 (MQ=255)
                             cATCGAAGACCTTGGCTCGGTGGCCGTCGACTCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTcgc                                                                                                             >  3:68501/1‑149 (MQ=255)
                                   aGACCTTTGCGCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCTAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGTAGGTGCCTCGCGGGTgg                                                                                                       <  1:58359/149‑1 (MQ=255)
                                      ccTTGGCTCGGTGGCCGTCGCCCCGGCCACCATTGCCATGGCCGCAGCGGAGTTCATCGTCGCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAg                                                                                                    >  5:212385/1‑149 (MQ=255)
                                       cTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGt                                                                                                                    <  6:243239/132‑1 (MQ=255)
                                       cTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGt                                                                                                                    >  5:243239/1‑132 (MQ=255)
                                       cTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGt                                                                                                   <  5:272714/149‑1 (MQ=255)
                                       cTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCACAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGt                                                                                                   >  4:236206/1‑149 (MQ=255)
                                              cGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAG‑CCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATg                                                                                           <  7:183478/149‑1 (MQ=255)
                                                     cGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAG‑CCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCAt                                                                                    >  8:180401/1‑149 (MQ=255)
                                                                  accaTTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCg                                                                        >  3:184925/1‑149 (MQ=255)
                                                                     aTTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAG‑CCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAg                                                                    <  7:180401/149‑1 (MQ=255)
                                                                           aTGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTggg                                                               >  7:154058/1‑149 (MQ=255)
                                                                           aTGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTggg                                                               <  4:52735/149‑1 (MQ=255)
                                                                            tGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTgggg                                                              <  4:184925/149‑1 (MQ=255)
                                                                              gCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTggggg                                                             <  4:76976/148‑1 (MQ=255)
                                                                                 gAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTAc                                                         <  4:49530/149‑1 (MQ=255)
                                                                                       gAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATg                                                                                           <  3:169030/109‑1 (MQ=255)
                                                                                       gAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATg                                                                                           >  4:169030/1‑109 (MQ=255)
                                                                                          ttCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGt                                                                                                                    >  7:179794/1‑81 (MQ=255)
                                                                                          ttCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGt                                                                                                                    <  8:179794/81‑1 (MQ=255)
                                                                                              tCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGt                                             >  3:10328/1‑148 (MQ=255)
                                                                                                   tcGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAg                                                                    <  4:245040/120‑1 (MQ=255)
                                                                                                   tcGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAg                                                                    >  3:245040/1‑120 (MQ=255)
                                                                                                    cGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAg                                                                    <  5:260470/119‑1 (MQ=255)
                                                                                                    cGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAg                                                                    >  6:260470/1‑119 (MQ=255)
                                                                                                               ggTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTAtgt                           <  8:154058/149‑1 (MQ=255)
                                                                                                                     aaGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCtgt                     >  1:146705/1‑149 (MQ=255)
                                                                                                                       ggACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTgg                   <  6:233139/149‑1 (MQ=255)
                                                                                                                        gACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTggg                  >  7:38358/1‑149 (MQ=255)
                                                                                                                         aCTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCtgt                     <  4:222283/145‑1 (MQ=255)
                                                                                                                         aCTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCtgt                     >  3:222283/1‑145 (MQ=255)
                                                                                                                           tGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTAt               >  2:255997/1‑149 (MQ=255)
                                                                                                                             ggACCTGTACCACAAGGCCCCCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTgggg                                                              <  1:12282/100‑1 (MQ=255)
                                                                                                                             ggACCTGTACCACAAGGCCCCCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTgggg                                                              >  2:12282/1‑100 (MQ=255)
                                                                                                                               aCCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCATCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCg           >  2:65402/1‑149 (MQ=255)
                                                                                                                                  tGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTgg                   <  1:231773/138‑1 (MQ=255)
                                                                                                                                  tGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGAAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTgg                   >  2:231773/1‑138 (MQ=255)
                                                                                                                                   gTACCACAAGGCCACCACAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCggg         >  2:103038/1‑147 (MQ=255)
                                                                                                                                   gTACCACAAGGCCACCACAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCggg         <  1:103038/147‑1 (MQ=255)
                                                                                                                                    tACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTAtg                            >  1:44416/1‑127 (MQ=255)
                                                                                                                                    tACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTAtg                            <  2:44416/127‑1 (MQ=255)
                                                                                                                                    tACCACAAGGCCACCACAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGt        <  3:236206/147‑1 (MQ=255)
                                                                                                                                        acaAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTAt               >  7:171130/1‑136 (MQ=255)
                                                                                                                                        acaAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTAt               <  8:171130/136‑1 (MQ=255)
                                                                                                                                        acaAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATc  <  2:146705/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
TCAACAAGCGTGGTGCGCAGCCTGCCGCGCATCGAAGACCTTGGCTCGGTGGCCGTCGACCCGGCCACCATTGCCATGGCCGAAGCGGAGTTCATCGTCTCGGGCGGCAACGGTGTCAAGGACTGGGACCTGTACCACAAGGCCACCGCAGCCCTCGGCGCTACCGAAGGTGCCTCGCGGGTGGCAGTGGACGATGGCTTCATGCCGCGCAACCGCCAGGTGGGGGCTACCGGTACCTGGGTTACCGCGCGTGTCTATGTGGCTGTGGGTATCTCGGGTGCGATC  >  NC_002947/375482‑375766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑