Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,245,073 G→A 8.0% E160E (GAG→GAA)  hmgR → Hmg transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,245,0730GA 8.0% 144.9 / 2.4 50E160E (GAG→GAA) hmgRHmg transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CTTCATCGCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATGCGCTGTCGGCGC  >  NC_002947/5244926‑5245221
                                                                                                                                                    |                                                                                                                                                    
ctTCATCGCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCcgagc                                                                                                                                                     >  3:78142/1‑149 (MQ=255)
 ttCATCGCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCcgagc                                                                                                                                                     >  8:89023/1‑148 (MQ=255)
     tCGCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCgaga                                                                                                                                                 >  4:205610/1‑148 (MQ=255)
       gCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAAc                                                                                                                                              >  4:193519/1‑149 (MQ=255)
         cGTGTGGGGCTACGAGGGGGCCAGGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCTGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCTCCGCCTACCTGCCCGAGCGAGAAAccc                                                                                                                                            <  3:173436/149‑1 (MQ=255)
         cGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGGCGCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAAcc                                                                                                                                             <  1:48520/149‑1 (MQ=255)
          gtgtGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCa                                                                                                                                           <  8:86200/149‑1 (MQ=255)
             tgGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAgg                                                                                                                                         <  1:28765/148‑1 (MQ=255)
                ggCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCt                                                                                                                                     >  6:36325/1‑149 (MQ=255)
                           ggCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAgg                                                                                                                           >  7:72473/1‑148 (MQ=255)
                                       aaGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCtt                                                                                                              >  4:238298/1‑149 (MQ=255)
                                          cATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGc                                                                                                           <  7:29569/149‑1 (MQ=255)
                                           aTCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGc                                                                                                           >  4:113815/1‑148 (MQ=255)
                                           aTCGAACCTGCCGTGCGCGCGGTCACAGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGc                                                                                                           >  3:147792/1‑148 (MQ=255)
                                             cGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGcaa                                                                                                         >  7:112023/1‑148 (MQ=255)
                                               aaCCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGcaaca                                                                                                       >  7:126094/1‑148 (MQ=255)
                                                     ccGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGc                                                                                                 <  3:113815/148‑1 (MQ=255)
                                                      cGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCa                                                                                               <  7:89023/149‑1 (MQ=255)
                                                          cgcgcgGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCCGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGa                                                                                           <  4:78142/149‑1 (MQ=255)
                                                             tgggTCACCACGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTa                                                                                        >  8:172051/3‑149 (MQ=255)
                                                             gcgGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTa                                                                                        <  5:36325/149‑1 (MQ=255)
                                                                gTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATca                                                                                     <  3:205610/149‑1 (MQ=255)
                                                                gTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATca                                                                                     <  3:193519/149‑1 (MQ=255)
                                                                         gtgACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACActgct                                                                            <  8:112023/149‑1 (MQ=255)
                                                                                      gttcggtGCTGCCGTTGCGCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCcgc                                                               <  8:78443/149‑1 (MQ=255)
                                                                                          ggtGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGaa                                                            <  8:72473/148‑1 (MQ=255)
                                                                                               tgcCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGc                                                      >  7:65062/1‑149 (MQ=255)
                                                                                                gcCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCttt                                                                                                             >  4:75286/1‑93 (MQ=255)
                                                                                                gcCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCttt                                                                                                             >  4:153816/1‑93 (MQ=255)
                                                                                                gcCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCttt                                                                                                             <  3:75286/93‑1 (MQ=255)
                                                                                                gcCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCttt                                                                                                             <  3:153816/93‑1 (MQ=255)
                                                                                                     tGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTg                                                   >  4:75102/1‑146 (MQ=255)
                                                                                                     tGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTg                                                   <  3:75102/146‑1 (MQ=255)
                                                                                                        tCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCAt                                             >  7:51450/1‑149 (MQ=255)
                                                                                                         cAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCAtg                                            >  3:218900/1‑149 (MQ=255)
                                                                                                          aGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCAtgt                                           <  3:238298/149‑1 (MQ=255)
                                                                                                           gTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCACCAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCAtgtg                                          >  8:211857/1‑149 (MQ=255)
                                                                                                               gTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATg                                      >  4:81264/1‑149 (MQ=255)
                                                                                                                  gctcGG‑GCAGGTAGGCGGCGAATACCAGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCCTGTGCATGGcc                                   >  4:155815/4‑149 (MQ=255)
                                                                                                                           tGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATg                           >  4:190532/1‑149 (MQ=255)
                                                                                                                                ttCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCggg                      >  1:253202/1‑149 (MQ=255)
                                                                                                                                 tCGCCGCCTACCTGCCCGAACGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAgg                                                        >  7:124578/1‑114 (MQ=255)
                                                                                                                                 tCGCCGCCTACCTGCCCGAACGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAgg                                                        <  8:121089/114‑1 (MQ=255)
                                                                                                                                 tCGCCGCCTACCTGCCCGAACGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAgg                                                        <  8:124578/114‑1 (MQ=255)
                                                                                                                                 tCGCCGCCTACCTGCCCGAACGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAgg                                                        >  7:121089/1‑114 (MQ=255)
                                                                                                                                      gccTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGa                >  3:41835/1‑149 (MQ=255)
                                                                                                                                       ccTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGAt               <  4:218900/149‑1 (MQ=255)
                                                                                                                                           ccTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGTACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGc                          <  1:219979/134‑1 (MQ=255)
                                                                                                                                           ccTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGc                          >  2:219979/1‑134 (MQ=255)
                                                                                                                                           ccTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATgcg            <  2:253202/148‑1 (MQ=255)
                                                                                                                                            cTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATGCGCt          >  5:201857/1‑149 (MQ=255)
                                                                                                                                               cccGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATGCGCTGTc       >  8:252795/1‑149 (MQ=255)
                                                                                                                                                    gcgagAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATGCGCTGTCGgcgc  <  3:190532/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTCATCGCCGTGTGGGGCAACCAGGGGGCCACGGTGGTAAGCATCGAACCTGCCGTGCGCGCGGTCACCGTGGTGACCCAGATCGGTTCGGTGCTGCCGTTGCTCAGTTCGTCCACCGG‑GCTGGTATTCGCCGCCTACCTGCCCGAGCGAGAAACCCAGGAGCTGCGCGACAGGGAACTGGCCGCTTTGCAACAAACCGCCAGTGACTATCACACACTGCTGGCCGGCATCCGCGAAAAGGGCCTGCACCATGTGCATGGCCTGCTGATGCCGGGGGTGGATGCGCTGTCGGCGC  >  NC_002947/5244926‑5245221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑