Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,259,675 G→A 8.1% A73T (GCT→ACT)  yqeF → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,259,6750GA 8.1% 139.9 / 2.5 49A73T (GCT→ACT) yqeFacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

AGTGCCTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCTTGCCC  >  NC_002947/5259527‑5259822
                                                                                                                                                    |                                                                                                                                                   
aGTGCCTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAgg                                                                                                                                                     <  3:160692/149‑1 (MQ=255)
 gTGCCTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCAAGGCAGCGATCAAGGc                                                                                                                                                    <  5:67145/149‑1 (MQ=255)
    ccTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTgg                                                                                                                                                 >  6:229529/1‑149 (MQ=255)
     cTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGc                                                                                                                                                >  2:196481/1‑149 (MQ=255)
        cGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTa                                                                                                                                             <  4:86366/149‑1 (MQ=255)
        cGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTa                                                                                                                                             >  3:175843/1‑149 (MQ=255)
          gTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTAcc                                                                                                                                           <  7:78752/149‑1 (MQ=255)
                   ggCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCg                                                                                                                                   >  3:190474/1‑148 (MQ=255)
                          ccgTGATCAAGCAACTGCTGAAACACACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAATTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGcc                                                                                                                           <  3:152964/149‑1 (MQ=255)
                          ccgTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGcc                                                                                                                           <  5:115710/149‑1 (MQ=255)
                                 cAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGccctgaccc                                                                                                                    >  6:241108/1‑149 (MQ=255)
                                 cAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGAGCAAGGCTGGCCTACCCTTCAGCGTACCGGccctgaccc                                                                                                                    >  6:76703/1‑149 (MQ=255)
                                  aaGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGccctgaccct                                                                                                                   <  8:153528/149‑1 (MQ=255)
                                      aaCTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGaa                                                                                                                >  4:198257/1‑148 (MQ=255)
                                         tgctgAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAg                                                                                                            <  1:131577/149‑1 (MQ=255)
                                           ctgAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAg                                                                                                            <  4:175843/147‑1 (MQ=255)
                                            tgAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTc                                                                                                         >  8:3142/1‑149 (MQ=255)
                                               aacaaacCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGc                                                                                                      <  5:229529/149‑1 (MQ=255)
                                                    acCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACACTGAACAAGGTCTGCGGCTc                                                                                                 >  6:167658/1‑149 (MQ=255)
                                                      cGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCgg                                                                                               <  3:198257/149‑1 (MQ=255)
                                                       gggCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCggg                                                                                              >  1:263087/1‑149 (MQ=255)
                                                        ggCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCTGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGc                                                                                             <  2:223089/149‑1 (MQ=255)
                                                        ggCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGc                                                                                             <  1:196481/149‑1 (MQ=255)
                                                          cTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCt                                                                                           <  6:78676/149‑1 (MQ=255)
                                                               cccGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGACTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAgg                                                                                      >  3:161729/1‑149 (MQ=255)
                                                               cccGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGACTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAgg                                                                                      >  3:165035/1‑149 (MQ=255)
                                                                        ggTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCAcc                                                                             <  4:190474/149‑1 (MQ=255)
                                                                         gTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGc                                                                                                                            <  8:118136/101‑1 (MQ=255)
                                                                          tCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGcc                                                                                                                           >  1:26171/1‑101 (MQ=255)
                                                                          tCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGcc                                                                                                                           <  2:26171/101‑1 (MQ=255)
                                                                                 aGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTgccgcc                                                                     >  7:247131/1‑148 (MQ=255)
                                                                                     aTCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCa                                                                                                                                      >  2:244359/1‑79 (MQ=255)
                                                                                     aTCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCa                                                                                                                                      <  1:244359/79‑1 (MQ=255)
                                                                                       ccTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGa                                                              >  8:42808/1‑149 (MQ=255)
                                                                                            gCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCg                                                          <  1:41716/148‑1 (MQ=255)
                                                                                            gCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCg                                                          >  2:41716/1‑148 (MQ=255)
                                                                                             ccAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCt                                                        >  5:59277/1‑149 (MQ=255)
                                                                                             ccAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCt                                                        <  2:263087/149‑1 (MQ=255)
                                                                                                       aCTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATg                                               <  6:113208/148‑1 (MQ=255)
                                                                                                           ccggtgccggtCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAg                                          >  4:10117/1‑149 (MQ=255)
                                                                                                            cggtgccggtCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAgg                                         <  7:42808/149‑1 (MQ=255)
                                                                                                               tgccggtCAGAACCCGGCACGCCAGGCAGCGATCAAGACTGGCCTACCCTTCAGCGTACCGGGCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAggtgg                                      <  4:165035/149‑1 (MQ=255)
                                                                                                               tgccggtCAGAACCCGGCACGCCAGGCAGCGATCAAGACTGGCCTACCCTTCAGCGTACCGGGCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAggtgg                                      <  4:161729/149‑1 (MQ=255)
                                                                                                                     tCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTTAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGCTCCGCTGTGGCGATGCCGAGGTGGTGATTg                                >  7:41569/1‑149 (MQ=255)
                                                                                                                             cGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGc                        <  2:206237/149‑1 (MQ=255)
                                                                                                                                    ccAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGTACCTTGCCGCCCAGGCGATCCGCTGCGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGga                   <  4:262851/147‑1 (MQ=255)
                                                                                                                                    ccAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGTACCTTGCCGACCAGGCGATCCGCTGAGGCGATGCCGAGGTGGTGATTGACGGTGGCCAGgaga                 >  3:262851/1‑149 (MQ=255)
                                                                                                                                               tCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCtt      <  8:247131/149‑1 (MQ=255)
                                                                                                                                                 aaGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCTTGc    >  6:212235/1‑149 (MQ=255)
                                                                                                                                                 aaGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCTTGc    >  1:135593/1‑149 (MQ=255)
                                                                                                                                                   ggCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCATGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCTTGccc  >  7:211293/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGTGCCTGCGGTTGACCTCGGCGCCGCCGTGATCAAGCAACTGCTGAAACAAACCGGGCTGGACCCGGCGCAGGTCGACGAAGTCATCCTCGGCCAAGTGCTCACTGCCGGTGCCGGTCAGAACCCGGCACGCCAGGCAGCGATCAAGGCTGGCCTACCCTTCAGCGTACCGGCCCTGACCCTGAACAAGGTCTGCGGCTCGGGCCTCAAGGCCCTGCACCTTGCCGCCCAGGCGATCCGCTGTGGCGATGCCGAGGTGGTGATTGCCGGTGGCCAGGAGAACATGAGCCTTGCCC  >  NC_002947/5259527‑5259822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑