Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,269,314 A→T 8.3% L342H (CTC→CAC) ‡ radA ← DNA repair enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,269,3140AT 8.3% 136.9 / 3.6 48L342H (CTC→CAC) ‡radADNA repair enzyme
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base T (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACG  >  NC_002947/5269166‑5269454
                                                                                                                                                    |                                                                                                                                            
cAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGa                                                                                                                                              <  1:10632/149‑1 (MQ=255)
     cATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCAcc                                                                                                                                         >  2:79652/1‑149 (MQ=255)
       tGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGc                                                                                                                                       <  1:69529/149‑1 (MQ=255)
          gCCCGTGGCCGGGTGCGCAAGCGCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGCTCCACCGCcat                                                                                                                                    <  4:19252/149‑2 (MQ=255)
          gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCCGCAGGGCCAGGTCCGAAGCCGGTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACAGCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGCTCCACCGCcat                                                                                                                                    >  3:19252/1‑148 (MQ=255)
          gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGCTCCACCGCcat                                                                                                                                    <  3:10479/149‑2 (MQ=255)
                   cGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGc                                                                                                                           <  2:27588/149‑1 (MQ=255)
                          gcAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACgg                                                                                                                    >  2:33383/1‑149 (MQ=255)
                           cAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGt                                                                                                                   >  2:210390/1‑149 (MQ=255)
                             aGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTc                                                                                                                 >  2:193977/1‑149 (MQ=255)
                             aGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTc                                                                                                                 <  1:210390/149‑1 (MQ=255)
                              gCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCACCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCt                                                                                                                >  4:79409/1‑149 (MQ=255)
                                tCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTgg                                                                                                              >  3:97708/1‑149 (MQ=255)
                                      tCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGCTAATCGTCCAgg                                                                                                        >  8:120607/1‑149 (MQ=255)
                                        aCCGCCGCCAGCAGGGTCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAgg                                                                                                        >  8:247703/1‑147 (MQ=255)
                                        aCCGCCGCCAGCAGGGTCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAgg                                                                                                        <  7:247703/147‑1 (MQ=255)
                                        aCCGCCGCCAGCAGGGCCAGGTCCGAAGTCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGcc                                                                                                      >  7:20034/1‑149 (MQ=255)
                                           gccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCaa                                                                                                   <  1:79652/149‑1 (MQ=255)
                                            ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                  >  8:108765/1‑149 (MQ=255)
                                            ccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCACCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTTGTCCAGGCCCAAg                                                                                                  <  3:79409/149‑1 (MQ=255)
                                                          aGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCgg                                                                                    <  7:42531/149‑1 (MQ=255)
                                                           ggTCCGAAGTCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCggg                                                                                   <  8:20034/149‑1 (MQ=255)
                                                           ggTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCggg                                                                                   <  1:193977/149‑1 (MQ=255)
                                                                       tttCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATgg                                                                       <  7:108765/149‑1 (MQ=255)
                                                                            aGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtc                                                                  >  2:82553/1‑149 (MQ=255)
                                                                               aCCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGCTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc                                                               <  7:166351/149‑1 (MQ=255)
                                                                                 cTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGa                                                             <  1:33383/149‑1 (MQ=255)
                                                                                       cccGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGCCGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCACATGGCTATCGTCGACCAgcg                                                       >  8:71787/1‑149 (MQ=255)
                                                                                       cccGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACAGCCATGGCGGTGGAGAACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCCCCGCGGGTTGGCCAGATTGCTATCGTCGACCAgc                                                        >  5:157693/1‑148 (MQ=255)
                                                                                        ccgccgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcgc                                                      >  2:186011/1‑149 (MQ=255)
                                                                                            cgACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGc                                                  <  1:186011/149‑1 (MQ=255)
                                                                                               ccACGTTGATTAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCAAAGGTTACCCGCCTCGGGTTGGCCAGATGGCTATCGTCTACCAGCGCCTGCAc                                                <  6:157693/148‑1 (MQ=255)
                                                                                                cACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgc                                                        <  5:154308/139‑1 (MQ=255)
                                                                                                cACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgc                                                        >  6:154308/1‑139 (MQ=255)
                                                                                                         aaaCACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCa                                     >  2:204540/1‑149 (MQ=255)
                                                                                                             acaTCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTgg                                 >  3:45543/1‑149 (MQ=255)
                                                                                                                  cTGGTCATGGGTAGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCgg                             <  7:120607/148‑1 (MQ=255)
                                                                                                                      tCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTAcc                        >  2:7822/1‑149 (MQ=255)
                                                                                                                           ggTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                            <  5:109489/108‑1 (MQ=255)
                                                                                                                           ggTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                            >  6:109489/1‑108 (MQ=255)
                                                                                                                           ggTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTcc                    >  6:260552/1‑148 (MQ=255)
                                                                                                                             tCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAg                 >  4:29622/1‑149 (MQ=255)
                                                                                                                                ggATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCCGCATTGGCCGGGTACCCTCCCAGGtt              <  1:7822/149‑1 (MQ=255)
                                                                                                                                 tccaCCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg             >  6:181828/4‑149 (MQ=255)
                                                                                                                                 gATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTg             >  7:136517/1‑149 (MQ=255)
                                                                                                                                    aCCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGcc           >  8:199861/1‑148 (MQ=255)
                                                                                                                                        ccATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACACGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTACCAGGTTGCCATcac      >  8:151825/1‑149 (MQ=255)
                                                                                                                                          aTGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca    <  5:260552/149‑1 (MQ=255)
                                                                                                                                            ggCGGTGCTCCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACg  >  5:113246/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACG  >  NC_002947/5269166‑5269454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑