Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,276,843 T→C 5.9% intergenic (‑147/‑197) cioA ← / → PP_4652 cyanide insensitive ubiquinol oxidase subunit I/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,276,8430TC 5.9% 204.6 / 2.2 68intergenic (‑147/‑197)cioA/PP_4652cyanide insensitive ubiquinol oxidase subunit I/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base C (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACA  >  NC_002947/5276698‑5276987
                                                                                                                                                 |                                                                                                                                                 
gTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGAc                                                                                                                                                <  7:228353/149‑1 (MQ=255)
  gCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGCACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGAcc                                                                                                                                               >  3:8284/1‑148 (MQ=255)
   gttCCTCTTCAGATAATACTGCGTGGGGGATTGTGGCACAAGTCGACTACTGCCCCCTCTGGGTAGAGTACGGCTTGTTTTAATTGTTCTGTTCGATCGCAGGGATTCCGTCCTGACGGCCAATTCATTGATCTGGAACGATTGAcca                                                                                                                                              <  5:104148/147‑2 (MQ=255)
      ccTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAg                                                                                                                                            <  5:183990/147‑1 (MQ=255)
            cAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatg                                                                                                                                     >  7:219535/1‑148 (MQ=255)
              gATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatgaat                                                                                                                                  >  7:195004/1‑149 (MQ=255)
               aTAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatgaatg                                                                                                                                 >  3:137847/1‑149 (MQ=255)
                  aTCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCt                                                                                                                               <  7:51623/148‑1 (MQ=255)
                  aTCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTa                                                                                                                              <  8:219535/149‑1 (MQ=255)
                            cGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAg                                                                                                                    >  4:196049/1‑149 (MQ=255)
                                    tgGCCCCAGTCGCCTACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGaa                                                                                                            <  7:131733/148‑1 (MQ=255)
                                    tgGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGaa                                                                                                            <  3:259295/148‑1 (MQ=255)
                                         ccAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCGATGAATGCTAGGAAGCATAGTC‑CCGAATGGccc                                                                                                      >  7:170154/1‑149 (MQ=255)
                                         ccAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTGCTGTTCGATCGCAGGGATTCCGGCCAGCCGCCCAATTCATTGATCTGGAACGCTTGAGCCCGATCAATGAATGCTAGGACGCATAGTC‑CCGAATGGcac                                                                                                      >  7:170156/1‑147 (MQ=255)
                                              cGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTgg                                                                                                 >  1:162953/1‑149 (MQ=255)
                                               gCCCCCTGCCCCCCCGGGGTCGAGTACGGCGTGGTGGAATTGTTCTGTTCGATCGCAGGGATTCCGTCCAGACGGCCAATTCATTGATCTGGAACGATTTACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTggg                                                                                                <  8:170156/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCGATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTggg                                                                                                <  8:170154/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTggg                                                                                                <  4:137847/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTAGATCGCAGGCATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTggg                                                                                                >  6:98544/1‑149 (MQ=255)
                                                cccACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGc                                                                                               >  8:145479/1‑149 (MQ=255)
                                                 ccACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGcc                                                                                              >  8:221969/1‑149 (MQ=255)
                                                         cccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCgtgggttg                                                                                      >  3:159211/1‑144 (MQ=255)
                                                         cccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTgtggtg                                                                                      <  7:221969/149‑1 (MQ=255)
                                                          ccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGtggtgg                                                                                     >  3:72049/1‑149 (MQ=255)
                                                          ccACTGGGTCGAGCACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGtggtgg                                                                                     <  4:8284/149‑1 (MQ=255)
                                                           cACTGAGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCATTGAATGCTAGGAAGCATAGTC‑GCGAATGGCCCGGTGGGCCGTGTGGTGGa                                                                                    <  6:15956/149‑1 (MQ=255)
                                                              tGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTg                                                                                 >  2:159500/1‑149 (MQ=255)
                                                                    gAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGt                                                                           >  3:110847/1‑149 (MQ=255)
                                                                    gAGTAAGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGt                                                                           <  8:247595/149‑1 (MQ=255)
                                                                     aGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTCGATTGCAGCGt                                                                           >  7:146605/1‑148 (MQ=255)
                                                                           gCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGt                                                                    >  7:79277/1‑149 (MQ=255)
                                                                              tgtTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGc                                                                 <  2:162953/149‑1 (MQ=255)
                                                                               gtTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGc                                                                 >  6:203799/1‑148 (MQ=255)
                                                                                   gAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAc                                                            <  4:72049/149‑1 (MQ=255)
                                                                                    aaTTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAcc                                                           >  6:268327/1‑149 (MQ=255)
                                                                                    aaTTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAcc                                                           >  6:180147/1‑149 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                   <  6:25951/136‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCGTCACCCTCtt                                                      <  4:110847/149‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCtt                                                      <  6:237613/149‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCtt                                                      >  8:206423/1‑149 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCAGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                   >  5:25951/1‑136 (MQ=255)
                                                                                           ctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCttt                                                     <  5:98544/148‑1 (MQ=255)
                                                                                           ctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCttt                                                     <  5:180147/148‑1 (MQ=255)
                                                                                            tgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTgtggtg                                                                                      <  8:97213/114‑1 (MQ=255)
                                                                                            tgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTgtggtg                                                                                      >  7:97213/1‑114 (MQ=255)
                                                                                                 gATCGCAGGGATTCCGGCCAGAAGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAg                                               <  7:206423/148‑1 (MQ=255)
                                                                                                   tCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAg                                            >  4:229020/1‑149 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGAt                                                  <  7:54650/141‑1 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGAt                                                  >  8:54650/1‑141 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGt                                           <  8:195004/148‑1 (MQ=255)
                                                                                                      cAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCATCACCCTCTTTGATTAGCAGTg                                          >  8:114356/1‑148 (MQ=255)
                                                                                                          gATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGaaaa                                     <  3:229020/149‑1 (MQ=255)
                                                                                                           aTTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAg                                    >  7:158487/1‑149 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGTATGGTAGGTAGGATAGTCTCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                   <  1:59313/115‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGcc                                <  3:196049/149‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGc                                 <  1:159500/148‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGc                                 <  2:189803/148‑1 (MQ=255)
                                                                                                                       cGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGTTGGATTGACGCGTGACCTGTTGCGTCAGCCTCTTTGATTAGCAGTGGAAAATTGGCGACATGAg                        >  8:193299/1‑149 (MQ=255)
                                                                                                                             aTTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATCGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACa                  <  7:145479/149‑1 (MQ=255)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCa                                             >  6:153279/1‑118 (MQ=38)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCa                                             <  5:153279/118‑1 (MQ=38)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                         <  8:234538/138‑1 (MQ=39)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                         >  7:234538/1‑138 (MQ=39)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGTTGGGCCGTGAGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGt      >  4:248915/1‑148 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                         <  5:73105/129‑1 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                         >  6:73105/1‑129 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGt      <  8:158487/148‑1 (MQ=255)
                                                                                                                                             cGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTTATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACa  <  7:193299/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTC‑CCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACA  >  NC_002947/5276698‑5276987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑