Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,276,846 C→T 6.2% intergenic (‑150/‑194) cioA ← / → PP_4652 cyanide insensitive ubiquinol oxidase subunit I/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,276,8460CT 6.2% 189.2 / 2.0 66intergenic (‑150/‑194)cioA/PP_4652cyanide insensitive ubiquinol oxidase subunit I/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

GTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGAT  >  NC_002947/5276698‑5276994
                                                                                                                                                    |                                                                                                                                                     
gTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGAc                                                                                                                                                       <  7:228353/149‑1 (MQ=255)
  gCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGCACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGAcc                                                                                                                                                      >  3:8284/1‑148 (MQ=255)
   gttCCTCTTCAGATAATACTGCGTGGGGGATTGTGGCACAAGTCGACTACTGCCCCCTCTGGGTAGAGTACGGCTTGTTTTAATTGTTCTGTTCGATCGCAGGGATTCCGTCCTGACGGCCAATTCATTGATCTGGAACGATTGAcca                                                                                                                                                     <  5:104148/147‑2 (MQ=255)
      ccTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAg                                                                                                                                                   <  5:183990/147‑1 (MQ=255)
            cAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatg                                                                                                                                            >  7:219535/1‑148 (MQ=255)
              gATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatgaat                                                                                                                                         >  7:195004/1‑149 (MQ=255)
               aTAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCaatgaatg                                                                                                                                        >  3:137847/1‑149 (MQ=255)
                  aTCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCt                                                                                                                                      <  7:51623/148‑1 (MQ=255)
                  aTCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTa                                                                                                                                     <  8:219535/149‑1 (MQ=255)
                            cGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAg                                                                                                                           >  4:196049/1‑149 (MQ=255)
                                    tgGCCCCAGTCGCCTACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGaa                                                                                                                   <  7:131733/148‑1 (MQ=255)
                                    tgGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGaa                                                                                                                   <  3:259295/148‑1 (MQ=255)
                                         ccAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCGATGAATGCTAGGAAGCATAGTCCCGAATGGccc                                                                                                             >  7:170154/1‑149 (MQ=255)
                                         ccAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTGCTGTTCGATCGCAGGGATTCCGGCCAGCCGCCCAATTCATTGATCTGGAACGCTTGAGCCCGATCAATGAATGCTAGGACGCATAGTCCCGAATGGcac                                                                                                             >  7:170156/1‑147 (MQ=255)
                                              cGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTgg                                                                                                        >  1:162953/1‑149 (MQ=255)
                                               gCCCCCTGCCCCCCCGGGGTCGAGTACGGCGTGGTGGAATTGTTCTGTTCGATCGCAGGGATTCCGTCCAGACGGCCAATTCATTGATCTGGAACGATTTACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTggg                                                                                                       <  8:170156/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCGATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTggg                                                                                                       <  8:170154/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTggg                                                                                                       <  4:137847/149‑1 (MQ=255)
                                               gCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTAGATCGCAGGCATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTggg                                                                                                       >  6:98544/1‑149 (MQ=255)
                                                cccACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGc                                                                                                      >  8:145479/1‑149 (MQ=255)
                                                 ccACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGcc                                                                                                     >  8:221969/1‑149 (MQ=255)
                                                         cccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCgtgggttg                                                                                             >  3:159211/1‑144 (MQ=255)
                                                         cccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTgtggtg                                                                                             <  7:221969/149‑1 (MQ=255)
                                                          ccACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGtggtgg                                                                                            >  3:72049/1‑149 (MQ=255)
                                                          ccACTGGGTCGAGCACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGtggtgg                                                                                            <  4:8284/149‑1 (MQ=255)
                                                           cACTGAGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCATTGAATGCTAGGAAGCATAGTCGCGAATGGCCCGGTGGGCCGTGTGGTGGa                                                                                           <  6:15956/149‑1 (MQ=255)
                                                              tGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTg                                                                                        >  2:159500/1‑149 (MQ=255)
                                                                    gAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGt                                                                                  >  3:110847/1‑149 (MQ=255)
                                                                    gAGTAAGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGt                                                                                  <  8:247595/149‑1 (MQ=255)
                                                                     aGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTCGATTGCAGCGt                                                                                  >  7:146605/1‑148 (MQ=255)
                                                                           gCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGt                                                                           >  7:79277/1‑149 (MQ=255)
                                                                              tgtTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGc                                                                        <  2:162953/149‑1 (MQ=255)
                                                                               gtTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGc                                                                        >  6:203799/1‑148 (MQ=255)
                                                                                   gAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAc                                                                   <  4:72049/149‑1 (MQ=255)
                                                                                    aaTTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAcc                                                                  >  6:180147/1‑149 (MQ=255)
                                                                                    aaTTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCAcc                                                                  >  6:268327/1‑149 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                          <  6:25951/136‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCGTCACCCTCtt                                                             <  4:110847/149‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCtt                                                             <  6:237613/149‑1 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCtt                                                             >  8:206423/1‑149 (MQ=255)
                                                                                         ttctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCAGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                          >  5:25951/1‑136 (MQ=255)
                                                                                           ctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCttt                                                            <  5:98544/148‑1 (MQ=255)
                                                                                           ctgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCttt                                                            <  5:180147/148‑1 (MQ=255)
                                                                                            tgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTgtggtg                                                                                             <  8:97213/114‑1 (MQ=255)
                                                                                            tgttcGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTgtggtg                                                                                             >  7:97213/1‑114 (MQ=255)
                                                                                                 gATCGCAGGGATTCCGGCCAGAAGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAg                                                      <  7:206423/148‑1 (MQ=255)
                                                                                                   tCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAg                                                   >  4:229020/1‑149 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGAt                                                         <  7:54650/141‑1 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGAt                                                         >  8:54650/1‑141 (MQ=255)
                                                                                                     gCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGt                                                  <  8:195004/148‑1 (MQ=255)
                                                                                                      cAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCATCACCCTCTTTGATTAGCAGTg                                                 >  8:114356/1‑148 (MQ=255)
                                                                                                          gATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGaaaa                                            <  3:229020/149‑1 (MQ=255)
                                                                                                           aTTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAg                                           >  7:158487/1‑149 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGTATGGTAGGTAGGATAGTCTCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGtt                                                                          <  1:59313/115‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGcc                                       <  3:196049/149‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGc                                        <  1:159500/148‑1 (MQ=255)
                                                                                                               cGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGc                                        <  2:189803/148‑1 (MQ=255)
                                                                                                                       cGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGTTGGATTGACGCGTGACCTGTTGCGTCAGCCTCTTTGATTAGCAGTGGAAAATTGGCGACATGAg                               >  8:193299/1‑149 (MQ=255)
                                                                                                                             aTTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATCGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACa                         <  7:145479/149‑1 (MQ=255)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCa                                                    >  6:153279/1‑118 (MQ=38)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCa                                                    <  5:153279/118‑1 (MQ=38)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                                >  7:234538/1‑138 (MQ=39)
                                                                                                                                 aTTGATCTGGGTCAATCGTTCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                                <  8:234538/138‑1 (MQ=39)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGTTGGGCCGTGAGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGt             >  4:248915/1‑148 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                                <  5:73105/129‑1 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGa                                >  6:73105/1‑129 (MQ=255)
                                                                                                                                          gAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGt             <  8:158487/148‑1 (MQ=255)
                                                                                                                                             cGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTTATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACa         <  7:193299/149‑1 (MQ=255)
                                                                                                                                                   aCCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAg    <  5:268327/148‑1 (MQ=255)
                                                                                                                                                    cccAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAgat  >  1:232882/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGCTTCCTCTTCAGATAATCCGGCGTCCGGGCTTGTGGCCCCAGTCGCCCACTGCCCCCACTGGGTCGAGTACGGCGTGTTGGAATTGTTCTGTTCGATCGCAGGGATTCCGGCCAGACGGCCAATTCATTGATCTGGAACGATTGACCCAGATCAATGAATGCTAGGAAGCATAGTCCCGAATGGCCCGGTGGGCCGTGTGGTGGATTGCCGCGTGACCTGTTGCCTCACCCTCTTTGATTAGCAGTGGAAAAGTGCCGACCTGAGCAAACAGTAACCGCTTGTTACAAACAGAT  >  NC_002947/5276698‑5276994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: