Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,307,341	C→G	10.5%	intergenic (‑90/+96)	recC ← / ← PP_5SG	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,307,341	0	C	G	10.5%	163.7 / 10.1	57	intergenic (‑90/+96)	recC/PP_5SG	multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base G (2/4);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.34e-01

GAGGCGGTTGCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGC  >  NC_002947/5307195‑5307488                                                                                                                                                    |                                                                                                                                                    gAGGCGGTTGCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGcc                                                                                                                                                    <  2:249015/148‑1 (MQ=255)     cGGTTGCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGcc                                                                                                                                               <  2:20545/149‑1 (MQ=255)      ggTTGCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGccc                                                                                                                                              >  6:223489/1‑149 (MQ=255)           ccGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTcc                                                                                                                                         <  1:105730/149‑1 (MQ=255)           ccGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTc                                                                                                                                          <  8:200450/148‑1 (MQ=255)             gTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGGCACGGCCGCTCCCg                                                                                                                                       <  7:59060/149‑1 (MQ=255)              tGGACGATCATGCAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGGCACGGCCGCTCCCGc                                                                                                                                      <  5:200138/149‑1 (MQ=255)                     tCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGAtt                                                                                                                               >  2:231904/1‑149 (MQ=255)                         gAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTcgc                                                                                                                            >  7:10031/1‑148 (MQ=255)                         gAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTcgc                                                                                                                            <  8:10031/148‑1 (MQ=255)                          aaGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTcgcg                                                                                                                           <  5:223489/148‑1 (MQ=255)                             ccAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAgg                                                                                                                       >  5:160567/1‑149 (MQ=255)                               aGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTc                                                                                                                     <  2:242256/149‑1 (MQ=255)                                gggTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGt                                                                                                                                                  >  5:121080/1‑119 (MQ=255)                                gggTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGt                                                                                                                                                  <  6:121080/119‑1 (MQ=255)                                    ggAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCTGGAGCGGCCGTGCCCTCTCCCGCAGCGATTCGCGCAGCTCGAGtt                                                                                                                >  8:188214/1‑149 (MQ=255)                                        aTGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGtttttt                                                                                                            >  3:179441/1‑149 (MQ=255)                                          gggCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTg                                                                                                           <  8:203966/148‑1 (MQ=255)                                            gCGGTGTTGATCATCGGAAGGTCTGTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACTTGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGccc                                                                                                        <  7:188214/149‑1 (MQ=255)                                                    gAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGa                                                                                                 <  4:179441/148‑1 (MQ=255)                                                      gCATCGTAAGGTCTCTGGAGGCGCTGATGGCGGATCGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACaa                                                                                              <  6:160567/149‑1 (MQ=255)                                                            gAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACAGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAAccc                                                                                         >  1:48937/1‑149 (MQ=255)                                                            gAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAAccc                                                                                         >  8:163290/1‑148 (MQ=255)                                                               ggTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTccc                                                                                                                                        >  6:46505/1‑98 (MQ=255)                                                               ggTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTccc                                                                                                                                        <  5:46505/98‑1 (MQ=255)                                                                       gAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCtgcgtgc                                                                             <  1:231904/149‑1 (MQ=255)                                                                            gcTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCtgcgtgcg                                                                            >  6:138254/1‑145 (MQ=255)                                                                                aTGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAgg                                                                    <  5:138254/149‑1 (MQ=255)                                                                                     tGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGttt                                                               >  1:186619/1‑149 (MQ=255)                                                                                         agGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGc                                                            >  3:136887/1‑148 (MQ=255)                                                                                           gggAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGa                                                          >  4:231214/1‑148 (MQ=255)                                                                                               aC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGGCACGGCCGCTCCCg                                                                                                                                       >  1:127676/1‑67 (MQ=25)                                                                                               aC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGGCACGGCCGCTCCCg                                                                                                                                       <  2:127676/67‑1 (MQ=25)                                                                                                c‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGaaa                                                        <  4:184339/145‑1 (MQ=255)                                                                                                c‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGaaa                                                        >  3:184339/1‑145 (MQ=255)                                                                                                  gATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAgg                                                                    >  2:12818/1‑132 (MQ=255)                                                                                                  gATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAgg                                                                    <  1:12818/132‑1 (MQ=255)                                                                                                  gATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGaa                                                    >  8:151177/1‑148 (MQ=255)                                                                                                      tCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCtt                                                <  8:145979/148‑1 (MQ=255)                                                                                                      tCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCtt                                                >  2:40557/1‑148 (MQ=255)                                                                                                        aCGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGAc                                             <  3:231214/149‑1 (MQ=255)                                                                                                        aCGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGAc                                             <  2:48937/149‑1 (MQ=255)                                                                                                                   aaaCGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTAc                                   >  2:270160/1‑148 (MQ=255)                                                                                                                     aCGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAg                                                                     >  7:116552/1‑112 (MQ=255)                                                                                                                     aCGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAg                                                                     <  8:116552/112‑1 (MQ=255)                                                                                                                        gCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGAc                                       <  1:249886/139‑1 (MQ=255)                                                                                                                        gCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGAc                                       >  2:249886/1‑139 (MQ=255)                                                                                                                         ccGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACaaa                                                                                                   >  3:225828/1‑78 (MQ=255)                                                                                                                         ccGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACaaa                                                                                                   <  4:225828/78‑1 (MQ=255)                                                                                                                                 cATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAggg                                                                   <  7:243262/102‑1 (MQ=255)                                                                                                                                 cATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAggg                                                                   >  8:243262/1‑102 (MQ=255)                                                                                                                                    cAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAAt                                                       >  4:194909/1‑111 (MQ=255)                                                                                                                                    cAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAAt                                                       <  3:194909/111‑1 (MQ=255)                                                                                                                                            gggAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTAc         >  8:114973/1‑149 (MQ=255)                                                                                                                                             ggAGCGGGCACGGCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTa          >  8:173216/1‑147 (MQ=255)                                                                                                                                             ggAGCGGGCACGGCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTa          <  7:173216/147‑1 (MQ=255)                                                                                                                                                gCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATc     <  4:136887/149‑1 (MQ=255)                                                                                                                                                   gCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGc  >  8:6888/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GAGGCGGTTGCCGTGGACGATCATGAAGCCAGGGTGGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAAC‑GATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGC  >  NC_002947/5307195‑5307488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑