| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,318,990 | G→C | 6.9% | H128Q (CAC→CAG) | ilvI ← | acetohydroxybutanoate synthase/acetolactate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,318,990 | 0 | G | C | 6.9% | 182.0 / 3.4 | 58 | H128Q (CAC→CAG) | ilvI | acetohydroxybutanoate synthase/acetolactate synthase |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base C (2/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01 | |||||||||||
ACGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATT > NC_002947/5318850‑5319133 | aCGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCAc > 1:203278/1‑149 (MQ=255) tATTCGAACTTCTCCGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGa > 2:126787/1‑148 (MQ=255) aTTCGAACTTCTCTGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGAt < 1:126787/148‑1 (MQ=255) aTTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGa < 4:201572/147‑1 (MQ=255) aTTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGa > 3:201572/1‑147 (MQ=255) ttCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCg > 6:132807/1‑149 (MQ=255) ttCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGATAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCg > 7:174038/1‑149 (MQ=255) gAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCtgt < 6:50765/134‑1 (MQ=255) gAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCtgt > 5:50765/1‑134 (MQ=255) aaCTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGATcaagaa < 3:95389/149‑6 (MQ=255) atccGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATa < 2:203278/146‑1 (MQ=255) gCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATAc > 2:62504/1‑148 (MQ=255) gTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCa < 4:212637/149‑1 (MQ=255) tCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCg < 5:132807/149‑1 (MQ=255) tCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCg > 8:124054/1‑149 (MQ=255) tCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGGTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTc > 1:213367/1‑149 (MQ=255) ttttGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑Ct > 5:263736/1‑149 (MQ=255) ggAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTgg > 7:26381/1‑147 (MQ=255) gAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa < 7:124054/148‑1 (MQ=255) aaTGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAgg < 8:26381/149‑1 (MQ=255) aaTGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAgg > 3:70367/1‑149 (MQ=255) cGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATc > 6:87644/1‑107 (MQ=255) cGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATc < 5:87644/107‑1 (MQ=255) gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCgg > 7:102129/1‑149 (MQ=255) gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCg < 4:70367/148‑1 (MQ=255) gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCg < 8:174038/148‑1 (MQ=255) cACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa < 6:140970/138‑1 (MQ=255) cACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACGTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa > 5:140970/1‑138 (MQ=255) cGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCACGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGccc > 1:77698/1‑149 (MQ=255) tggacctggacGTCCGGATTGCGGCAGGTAGAAAGCTTTTTTCAGAACTTCAGGTATCTCGGTCTGATTCTTGATCATGAAGCTGTGCt < 3:101708/89‑1 (MQ=255) tggacctggacGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTCATCATGAAGCTGTGCt > 4:101708/1‑89 (MQ=255) gacgacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATg > 8:101292/1‑143 (MQ=255) gacgacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATg < 7:101292/143‑1 (MQ=255) gacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCgaga > 7:43838/1‑96 (MQ=255) gacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCgaga < 8:43838/96‑1 (MQ=255) cgcCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGa > 3:77496/1‑110 (MQ=255) cgcCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGa < 4:77496/110‑1 (MQ=255) aGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg > 4:67301/1‑149 (MQ=255) ggTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGATTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg > 2:130249/1‑148 (MQ=255) gAAAGCTTTTTTCCGAACTTCAGGGATCTCGGTCGGATTCTTGCTCATGCAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa < 7:108761/149‑1 (MQ=255) gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa > 8:143947/1‑149 (MQ=255) gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa > 2:221846/1‑149 (MQ=255) gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa > 6:26393/1‑149 (MQ=255) ttttttCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg > 6:225864/1‑149 (MQ=255) ttCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGaa < 1:130249/148‑1 (MQ=255) ttCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCa > 8:184788/1‑149 (MQ=255) cAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCAt < 1:62504/148‑1 (MQ=255) cAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATg > 7:19972/1‑149 (MQ=255) gATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAgg < 6:263736/149‑1 (MQ=255) ctcGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg > 2:43243/1‑128 (MQ=255) ctcGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg < 1:43243/128‑1 (MQ=255) ggtcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTgg < 3:67301/149‑1 (MQ=255) ggtcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTg < 5:26393/148‑1 (MQ=255) tcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGc > 1:116952/1‑148 (MQ=255) ggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg < 1:156176/110‑1 (MQ=255) ggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg > 2:156176/1‑110 (MQ=255) ccgATCGTGAAGCACAGCTTCATGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCgg > 4:19206/3‑148 (MQ=255) ttaTCATTAAGCTGGGATTCACGATCGGCCGCGATATACCAATCATGTCGTTAACTCTGGAACCCCATAGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCgg < 1:221846/146‑1 (MQ=255) tCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAAt < 8:19972/148‑1 (MQ=255) aaGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCAtt < 5:225864/149‑1 (MQ=255) | ACGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATT > NC_002947/5318850‑5319133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |