Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,318,998	C→T	7.0%	V126M (GTG→ATG)	ilvI ←	acetohydroxybutanoate synthase/acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,318,998	0	C	T	7.0%	166.0 / 2.4	57	V126M (GTG→ATG)	ilvI	acetohydroxybutanoate synthase/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base T (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCG  >  NC_002947/5318850‑5319140                                                                                                                                                     |                                                                                                                                                aCGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCAc                                                                                                                                                 >  1:203278/1‑149 (MQ=255)    tATTCGAACTTCTCCGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGa                                                                                                                                               >  2:126787/1‑148 (MQ=255)     aTTCGAACTTCTCTGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGAt                                                                                                                                              <  1:126787/148‑1 (MQ=255)     aTTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGa                                                                                                                                               <  4:201572/147‑1 (MQ=255)     aTTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGa                                                                                                                                               >  3:201572/1‑147 (MQ=255)      ttCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCg                                                                                                                                            >  6:132807/1‑149 (MQ=255)      ttCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGATAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCg                                                                                                                                            >  7:174038/1‑149 (MQ=255)          aaCTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCGTGAAGCACAGCTTCATGATcaagaa                                                                                                                                        <  3:95389/149‑6 (MQ=255)                  atccGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATa                                                                                                                                 <  2:203278/146‑1 (MQ=255)                   gCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATAc                                                                                                                                >  2:62504/1‑148 (MQ=255)                        gTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCa                                                                                                                          <  4:212637/149‑1 (MQ=255)                             tCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCg                                                                                                                     <  5:132807/149‑1 (MQ=255)                             tCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCg                                                                                                                     >  8:124054/1‑149 (MQ=255)                                  tCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGGTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTc                                                                                                                >  1:213367/1‑149 (MQ=255)                                    ttttGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑Ct                                                                                                             >  5:263736/1‑149 (MQ=255)                                        ggAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTgg                                                                                                           >  7:26381/1‑147 (MQ=255)                                         gAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa                                                                                                         <  7:124054/148‑1 (MQ=255)                                          aaTGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAgg                                                                                                       >  3:70367/1‑149 (MQ=255)                                          aaTGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAgg                                                                                                       <  8:26381/149‑1 (MQ=255)                                               cGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATc                                                                                                                                             >  6:87644/1‑107 (MQ=255)                                               cGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATc                                                                                                                                             <  5:87644/107‑1 (MQ=255)                                                gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCgg                                                                                                 >  7:102129/1‑149 (MQ=255)                                                gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCg                                                                                                  <  8:174038/148‑1 (MQ=255)                                                gACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCg                                                                                                  <  4:70367/148‑1 (MQ=255)                                                   cACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa                                                                                                         <  6:140970/138‑1 (MQ=255)                                                   cACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACGTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGaa                                                                                                         >  5:140970/1‑138 (MQ=255)                                                     cGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCACGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGccc                                                                                            >  1:77698/1‑149 (MQ=255)                                                                 gacgacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATg                                                                                      >  8:101292/1‑143 (MQ=255)                                                                 gacgacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATg                                                                                      <  7:101292/143‑1 (MQ=255)                                                                    gacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCgaga                                                                                                                                   >  7:43838/1‑96 (MQ=255)                                                                    gacCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCgaga                                                                                                                                   <  8:43838/96‑1 (MQ=255)                                                                              cgcCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGa                                                                                                          <  4:77496/110‑1 (MQ=255)                                                                              cgcCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGa                                                                                                          >  3:77496/1‑110 (MQ=255)                                                                                  aGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg                                                               >  4:67301/1‑149 (MQ=255)                                                                                   ggTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGATTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg                                                               >  2:130249/1‑148 (MQ=255)                                                                                       gAAAGCTTTTTTCCGAACTTCAGGGATCTCGGTCGGATTCTTGCTCATGCAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa                                                          <  7:108761/149‑1 (MQ=255)                                                                                       gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa                                                          >  6:26393/1‑149 (MQ=255)                                                                                       gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa                                                          >  8:143947/1‑149 (MQ=255)                                                                                       gAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGa                                                          >  2:221846/1‑149 (MQ=255)                                                                                             ttttttCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg                                                    >  6:225864/1‑149 (MQ=255)                                                                                                 ttCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGaa                                                 <  1:130249/148‑1 (MQ=255)                                                                                                         ttCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCa                                        >  8:184788/1‑149 (MQ=255)                                                                                                           cAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCAt                                       <  1:62504/148‑1 (MQ=255)                                                                                                           cAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATg                                      >  7:19972/1‑149 (MQ=255)                                                                                                               gATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAgg                                  <  6:263736/149‑1 (MQ=255)                                                                                                                  ctcGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg                                                    >  2:43243/1‑128 (MQ=255)                                                                                                                  ctcGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCg                                                    <  1:43243/128‑1 (MQ=255)                                                                                                                     ggtcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTgg                            <  3:67301/149‑1 (MQ=255)                                                                                                                     ggtcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTg                             <  5:26393/148‑1 (MQ=255)                                                                                                                       tcggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGc                           >  1:116952/1‑148 (MQ=255)                                                                                                                         ggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg                                                               >  2:156176/1‑110 (MQ=255)                                                                                                                         ggATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAg                                                               <  1:156176/110‑1 (MQ=255)                                                                                                                               ccgATCGTGAAGCACAGCTTCATGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCgg                   >  4:19206/3‑148 (MQ=255)                                                                                                                                ttaTCATTAAGCTGGGATTCACGATCGGCCGCGATATACCAATCATGTCGTTAACTCTGGAACCCCATAGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCgg                   <  1:221846/146‑1 (MQ=255)                                                                                                                                   tCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAAt               <  8:19972/148‑1 (MQ=255)                                                                                                                                        aaGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCAtt         <  5:225864/149‑1 (MQ=255)                                                                                                                                              tgCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGt      <  2:116952/146‑1 (MQ=255)                                                                                                                                                cTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTcgcg  <  2:213367/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                ACGTATTCGAACTTCTCAGCCGGGTTGGTCATATCTTTTGGAATGTCGACCACGACTGGACCTGGACGACCGGATTGCGCCAGGTAGAAAGCTTTTTTCAGAACTTCAGGGATCTCGGTCGGATTCTTGATCATGAAGCTGTGCTTCACGATCGGCCGCGAGATACCAATCATGTCGGTTTC‑CTGGAAGGCATCGGTGCCCACCATGGTGCTAGGCACCTGGCCGGACAGGATGACCATCGGAATCGAGTCCATGTAGGCGGTGGCAATACCGGTAATGGCATTGGTCGCG  >  NC_002947/5318850‑5319140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑