Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,337,381 T→C 8.5% G277G (GGT→GGC)  PP_4696 → nitrogen regulation protein NR(I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,337,3810TC 8.5% 127.5 / 2.6 47G277G (GGT→GGC) PP_4696nitrogen regulation protein NR(I)
Reads supporting (aligned to +/- strand):  ref base T (19/24);  new base C (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ACCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGT  >  NC_002947/5337238‑5337528
                                                                                                                                               |                                                                                                                                                   
aCCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGt                                                                                                                                                <  7:244618/149‑1 (MQ=255)
  cGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCTAGATACGCCGGGTAGGTTCg                                                                                                                                                  <  8:139327/145‑1 (MQ=255)
  cGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCAAGATACGCCGGGTAGGTTCg                                                                                                                                                  >  7:139327/1‑145 (MQ=255)
      gcCAGCGCCGGGCGCGCGGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTc                                                                                                                                          <  2:90869/149‑1 (MQ=255)
          gcgcCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACCCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCa                                                                                                                                       >  1:68076/1‑148 (MQ=255)
            gcCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACCCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCaaaa                                                                                                                                    <  2:68076/149‑1 (MQ=255)
                ggCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTc                                                                                                                                <  4:236718/149‑1 (MQ=255)
                              gtggAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATTTgcgtatccg                                                                                                                  >  7:203230/1‑143 (MQ=255)
                               tggAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCGGTCGCAAAAGGTCGATGTGCGCCTGATc                                                                                                                 >  3:145375/1‑149 (MQ=255)
                                 gAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCGGTCGCAAAAGGTCGATGTGCGCCTGATcgc                                                                                                               <  4:145375/149‑1 (MQ=255)
                                 gAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATcgc                                                                                                               >  2:127607/1‑149 (MQ=255)
                                    gccgccGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATcgccgc                                                                                                            >  2:80529/1‑149 (MQ=255)
                                                     gCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGa                                                                                           <  2:138750/149‑1 (MQ=255)
                                                       tGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGCTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGaa                                                                                          >  2:182013/1‑148 (MQ=255)
                                                       tGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGCTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGaa                                                                                          >  2:122892/1‑148 (MQ=255)
                                                        gTTACTCGATGAAATTGGCGAACTCCCGCTCGAAGCCCACTCTAGCCTGCTGCGTGTGCTGCAGGAAGCCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTCCGCCTGCAAGCCGAGACCCACCGCGACCTGaaa                                                                                         >  5:252246/1‑148 (MQ=255)
                                                         ttCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGaaaaa                                                                                       <  5:105727/149‑1 (MQ=255)
                                                           ccTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAAcc                                                                                     >  7:18490/1‑149 (MQ=255)
                                                               gATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                   <  6:80790/147‑1 (MQ=255)
                                                               gATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTg                                                                                   <  6:196712/147‑1 (MQ=255)
                                                               gATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGc                                                                                 >  6:5319/1‑149 (MQ=255)
                                                               gATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGc                                                                                 >  8:243461/1‑149 (MQ=255)
                                                                        ggCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccg                                                                         <  1:127607/148‑1 (MQ=255)
                                                                        ggCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAggccg                                                                         <  5:5319/148‑1 (MQ=255)
                                                                                 ccGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTcc                                                                <  8:18490/148‑1 (MQ=255)
                                                                                  cGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGt                                                              <  4:72769/149‑1 (MQ=255)
                                                                                       gAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGa                                                         >  1:218338/1‑149 (MQ=255)
                                                                                       gAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGa                                                         <  7:243461/149‑1 (MQ=255)
                                                                                              aGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTAt                                                  <  8:70507/149‑1 (MQ=255)
                                                                                                  tCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGtt                                                                  >  5:201899/1‑129 (MQ=255)
                                                                                                  tCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGtt                                                                  <  6:201899/129‑1 (MQ=255)
                                                                                                         ctgcGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCa                                       <  2:218338/149‑1 (MQ=255)
                                                                                                             gtgtGCTGCAGGAAGGCGTGATACGCCGTGTATGTTCGGTGGAGTCGCAAAAGGTCGATGTTCGCCTGATGGCCGCTACCCACGGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCTTGAAGACTTGTATTACCGACTGCACGTc                                   <  6:252246/149‑1 (MQ=255)
                                                                                                                   tgcGGGACGGCGAGGTGCGCCGGGTGGGTTCGGTGCAGTCGCAAAGGGGCGATGTGCGCCTGCTCGCCGCGGGCCCGCGGGACCTGAAAAACCTGGCCCGGGCCGGCCAGTTCCGTGAGGAGGTGTAGTAGGGCCTGCACGGCCTCggg                             >  6:178489/1‑147 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTAc                                               >  4:138962/1‑126 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTAc                                               <  3:138962/126‑1 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCAc                                      >  2:119274/1‑135 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCAc                                      <  1:119274/135‑1 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGCTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGaa                        <  1:122892/149‑1 (MQ=255)
                                                                                                                        gAAGGCGAGATACGCCGGGTAGGCTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGaa                        <  1:182013/149‑1 (MQ=255)
                                                                                                                              gagaTACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGcc                  >  3:82930/1‑149 (MQ=255)
                                                                                                                                      ccGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTgc          <  5:4857/149‑1 (MQ=255)
                                                                                                                                         ggTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGGGGCCCGCCGCGGCGTGAAAAACCTGGCGAAGGCCGGCCAGTGGGGTGAAGGCTGGTTTGAGCGACTGGCCGTCATGGCCCGGAGGCTGCCGGCCGTGcgcg       >  6:94391/1‑149 (MQ=255)
                                                                                                                                          gTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCGCACGGCCCTgcgc        >  1:228049/1‑147 (MQ=255)
                                                                                                                                           tAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAg     >  6:232468/1‑149 (MQ=255)
                                                                                                                                              gTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGt  <  7:63552/149‑1 (MQ=255)
                                                                                                                                              gTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGt  <  4:82930/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ACCGGCGCCAGCGCCGGGCGCGCCGGCTTGGTGGAGGCCGCCGATGGCGGTACGCTGTTCCTCGATGAAATTGGCGAACTGCCGCTCGAAGCCCAGGCTCGCCTGCTGCGTGTGCTGCAGGAAGGCGAGATACGCCGGGTAGGTTCGGTGCAGTCGCAAAAGGTCGATGTGCGCCTGATCGCCGCGACCCACCGCGACCTGAAAAACCTGGCCAAGGCCGGCCAGTTCCGTGAAGACTTGTATTACCGACTGCACGTCATCGCCCTGAAGCTGCCGGCCCTGCGCGAGCGT  >  NC_002947/5337238‑5337528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑