Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,346,338 T→C 7.8% V579A (GTC→GCC)  acsA‑II → acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,346,3380TC 7.8% 141.0 / 3.0 51V579A (GTC→GCC) acsA‑IIacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATAC  >  NC_002947/5346200‑5346476
                                                                                                                                          |                                                                                                                                          
tgctgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGACCAGGGCCAGCagctcg                                                                                                                                  <  8:3332/149‑6 (MQ=255)
   tgcATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc                                                                                                                               >  3:39835/1‑149 (MQ=255)
     cATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc                                                                                                                               <  1:140020/147‑1 (MQ=255)
     cATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATc                                                                                                                               <  5:121088/147‑1 (MQ=255)
       tGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCgg                                                                                                                             >  2:99896/1‑147 (MQ=255)
             cGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCg                                                                                                                     >  7:118912/1‑149 (MQ=255)
                    gAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGcc                                                                                                              >  1:210210/1‑149 (MQ=255)
                    gAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGcc                                                                                                              >  3:183010/1‑149 (MQ=255)
                         cgcgGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGc                                                                                                         <  4:153628/149‑1 (MQ=255)
                                 tCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGACCAGGGCCAGCag                                                                                                                                      >  6:225571/1‑111 (MQ=255)
                                 tCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGACCAGGGCCAGCag                                                                                                                                      <  5:225571/112‑2 (MQ=255)
                                   ggCTACCCCCACGTCCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGt                                                                                                <  3:147031/148‑1 (MQ=255)
                                   ggCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTg                                                                                               <  1:99896/149‑1 (MQ=255)
                                   ggCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTg                                                                                               <  8:176960/149‑1 (MQ=255)
                                    gCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTgg                                                                                              <  3:212847/149‑1 (MQ=255)
                                    gCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTgg                                                                                              >  4:212847/1‑149 (MQ=255)
                                        cccccACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgcgc                                                                                          <  6:31579/149‑1 (MQ=255)
                                         ccccACGACCTCAACGGCCAAGTCGTCTACGCCTTCGTCACGACAAAGAATCGCGTGACACAAGATGACACCCTCAAGGCCGAGATGCTGGCCCTGGTCAGCAAGGAAATCGCCAGCTTCGCAAACCCTGAGATGATTCAGACGgcgc                                                                                          >  4:182401/1‑148 (MQ=255)
                                                  cTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAcc                                                                                >  5:183567/1‑149 (MQ=255)
                                                    cAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAccc                                                                               >  1:121706/1‑148 (MQ=255)
                                                      aGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAg                                                                                                          >  3:88292/1‑119 (MQ=255)
                                                      aGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAg                                                                                                          <  4:88292/119‑1 (MQ=255)
                                                           cAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGAcgcg                                                                       >  8:230488/1‑149 (MQ=255)
                                                            aaGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACgcgc                                                                      <  2:121706/149‑1 (MQ=255)
                                                               gCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCa                                                                   <  7:230488/149‑1 (MQ=255)
                                                                    tACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGc                                                                >  7:144910/1‑147 (MQ=255)
                                                                         cTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCa                                                         >  2:139473/1‑149 (MQ=255)
                                                                           tCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATg                                                       >  3:239646/1‑149 (MQ=255)
                                                                              tCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATgcgg                                                    <  7:123691/149‑1 (MQ=255)
                                                                                aCGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATgcggcg                                                  >  7:77275/1‑149 (MQ=255)
                                                                                    ccATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGtata                                              <  3:40253/149‑1 (MQ=255)
                                                                                      aTGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACt                                            <  6:183567/149‑1 (MQ=255)
                                                                                       tGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTg                                           <  4:39835/149‑1 (MQ=255)
                                                                                         aaTGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgcg                                         <  2:210210/149‑1 (MQ=255)
                                                                                         aaTGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgc                                          <  4:239646/148‑1 (MQ=255)
                                                                                           tGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGa                                                            <  7:206175/128‑1 (MQ=255)
                                                                                           tGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGa                                                            >  8:206175/1‑128 (MQ=255)
                                                                                                  acacCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGc                                <  8:144910/149‑1 (MQ=255)
                                                                                                   cacCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGcc                               <  3:177499/149‑1 (MQ=255)
                                                                                                    acCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCt                              >  8:82883/1‑149 (MQ=255)
                                                                                                      cAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTg                             >  8:84266/1‑148 (MQ=255)
                                                                                                        gATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgc                                          <  1:9642/133‑1 (MQ=255)
                                                                                                        gATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTgc                                          >  2:9642/1‑133 (MQ=255)
                                                                                                                   cAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGaa                >  5:179062/1‑148 (MQ=255)
                                                                                                                    aaGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGaaaa              <  7:84266/149‑1 (MQ=255)
                                                                                                                    aaGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGaaaa              >  3:74047/1‑149 (MQ=255)
                                                                                                                         cGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTgg         <  2:21593/149‑1 (MQ=255)
                                                                                                                          tccttgctgACCAGGGCCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTggg        >  7:212714/5‑149 (MQ=255)
                                                                                                                          gAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTggg        <  8:77275/149‑1 (MQ=255)
                                                                                                                           aGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGc       <  1:8167/149‑1 (MQ=255)
                                                                                                                                ctgGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATAc  >  5:193454/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTCAGGCAAGATCATGCGGCGTATACTGCGCAAAATCGCCTGCAATGAGCTGGAAAACCTGGGCGATAC  >  NC_002947/5346200‑5346476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑