Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,372,335 A→G 7.1% S279P (TCC→CCC) ‡ carA ← carbamoyl‑phosphate synthase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,372,3350AG 7.1% 160.4 / 2.6 57S279P (TCC→CCC) ‡carAcarbamoyl‑phosphate synthase small subunit
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CGAACGTTGCCCGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGAACACGCCGTCCGGGTTGAGTGC  >  NC_002947/5372193‑5372482
                                                                                                                                              |                                                                                                                                                    
cgAACGTTGCCCGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAg                                                                                                                                               >  8:251869/1‑149 (MQ=255)
        gCCCGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGAGCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCcagc                                                                                                                                        <  8:122496/148‑1 (MQ=255)
         cccGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCcagcag                                                                                                                                      >  1:158639/1‑149 (MQ=255)
          ccGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGTCCGGCGC‑Caaga                                                                                                                                             <  4:198089/141‑4 (MQ=255)
          ccGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGTCCGGCGC‑Caaga                                                                                                                                             >  3:198089/1‑138 (MQ=255)
          ccGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCagcagc                                                                                                                                     >  2:115516/1‑149 (MQ=255)
            ggCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCtg                                                                                                                                   >  2:5727/1‑149 (MQ=255)
              cAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCtggt                                                                                                                                 >  1:157217/1‑149 (MQ=255)
                  gTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGcc                                                                                                                             >  4:158984/1‑149 (MQ=255)
                             aaCGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCcagcag                                                                                                                                      <  8:172220/129‑1 (MQ=255)
                             aaCGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCcagcag                                                                                                                                      >  7:172220/1‑129 (MQ=255)
                              aCGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGcc                                                                                                                 >  2:224497/1‑149 (MQ=255)
                                ggCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCtggt                                                                                                                                 <  1:182189/131‑1 (MQ=255)
                                ggCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCtggt                                                                                                                                 >  2:182189/1‑131 (MQ=255)
                                       ccGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCgg                                                                                                        <  4:26887/149‑1 (MQ=255)
                                        cGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCggg                                                                                                       <  3:158984/149‑1 (MQ=255)
                                         gTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGa                                                                                                      <  8:16745/149‑1 (MQ=255)
                                            gTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGAtct                                                                                                   >  5:227974/1‑149 (MQ=255)
                                              tCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCg                                                                                                 >  5:72276/1‑149 (MQ=255)
                                               ctggctggTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCg                                                                                                 <  2:157217/148‑1 (MQ=255)
                                                  gctggTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCgg                                                                                                <  2:158639/146‑1 (MQ=255)
                                                  gctggTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTCGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGttt                                                                                             <  3:204105/149‑1 (MQ=255)
                                                       tGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAg                                                                                         >  7:136061/1‑148 (MQ=255)
                                                            aTGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAgg                                                                                                                                              >  4:89252/1‑90 (MQ=255)
                                                            aTGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAgg                                                                                                                                              <  3:89252/90‑1 (MQ=255)
                                                            aTGACCACACCAGTATCCAGGTCCTGGACCGGGTGGATGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGAttt                                                                                    >  2:203517/1‑148 (MQ=255)
                                                               aCCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCtt                                                                                <  8:136061/149‑1 (MQ=255)
                                                                  acacCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGAt                                                                             <  8:192371/149‑1 (MQ=255)
                                                                    acCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCg                                                                                                                            <  6:178484/100‑1 (MQ=255)
                                                                    acCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCg                                                                                                                            >  5:178484/1‑100 (MQ=255)
                                                                       aGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCt                                                                        <  1:115516/149‑1 (MQ=255)
                                                                        gTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTg                                                                       <  7:251869/149‑1 (MQ=255)
                                                                           tCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGa                                                                                                      >  7:129589/1‑115 (MQ=255)
                                                                           tCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGa                                                                                                      <  8:129589/115‑1 (MQ=255)
                                                                           tCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGAt                                                                    >  2:173857/1‑149 (MQ=255)
                                                                           tCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGAt                                                                    >  7:106295/1‑149 (MQ=255)
                                                                             cAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATc                                                                   <  1:5727/148‑1 (MQ=255)
                                                                                 tCCTGGACCGGGTGGATGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTa                                                              <  1:203517/149‑1 (MQ=255)
                                                                                   cTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCt                                                                        <  8:123215/137‑1 (MQ=255)
                                                                                   cTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCt                                                                        >  7:123215/1‑137 (MQ=255)
                                                                                                ttGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAgg                                               <  1:173857/149‑1 (MQ=255)
                                                                                                      ccGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCAcc                                         <  1:90804/149‑1 (MQ=255)
                                                                                                       cgggggggccggggccCATTTTCCGGGTCTTGGGCCCGTCCCCACTGGTTTAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCAcc                                         <  1:224497/143‑1 (MQ=255)
                                                                                                           gtggccgtggccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCAc                                          <  4:29778/143‑1 (MQ=255)
                                                                                                           gtggccgtggccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCAc                                          >  3:29778/1‑143 (MQ=255)
                                                                                                                cgtggccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCCCAAGGGCCGTTg                               >  2:87560/1‑149 (MQ=255)
                                                                                                                 gtggccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAg                                                             <  2:75988/118‑1 (MQ=255)
                                                                                                                 gtggccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAg                                                             >  1:75988/1‑118 (MQ=255)
                                                                                                                    gccCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGAAGCCGAATACCCGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGAAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGa                             >  5:170764/1‑147 (MQ=255)
                                                                                                                     cccATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAg                          >  2:251220/1‑149 (MQ=255)
                                                                                                                      ccATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAg                          >  2:143221/1‑148 (MQ=255)
                                                                                                                                 gccctGGCGTCCGGCGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGaa                       >  6:104607/5‑140 (MQ=255)
                                                                                                                                 gccctGGCGTCCGGCGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGaa                       <  5:104607/136‑1 (MQ=255)
                                                                                                                                      ggCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGAACACGCCGTCCgg           <  6:72276/147‑1 (MQ=255)
                                                                                                                                       gcgcCGGAAGC‑CAGGGCCAGCAGCTGGTGGACGAGGCACATGCCGAAAACCGGGAACTCGGTTTCGAGCATTTCCTTGAAAGCCTGCATCGCGTAGTCGCACGGCTCAGGGTCACCAGCGCCGTTGGACAGGAACACGCCGACCGGCtt        >  1:141569/1‑149 (MQ=255)
                                                                                                                                           cGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAg                          <  6:179166/127‑1 (MQ=255)
                                                                                                                                           cGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAg                          >  5:179166/1‑127 (MQ=255)
                                                                                                                                              aCGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGAACACGCCGTCCGGGTTGAGTGc  >  3:164363/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGAACGTTGCCCGGCAGGGTCGCCTCGTCAACGGCGAAACCGTGGTTCTGGCTGGTGATCATGACCACACCAGTATCCAGGTCCTGGACCGGGTGGTTGGCACCGTGGTGGCCGTGGCCCATTTTCACGGTCTTGGCGCCGGACGC‑CAGGGCCAGCAGCTGGTGGCCGAGGCAGATGCCGAATACCGGGATCTCGGTTTCGAGGATTTCCTTGATCGCCTGGATCGCGTAGTCGCACGGCTCAGGGTCACCAGGGCCGTTGGACAGGAACACGCCGTCCGGGTTGAGTGC  >  NC_002947/5372193‑5372482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑