Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,384,528 G→C 10.4% G331A (GGC→GCC) ‡ lldP → L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,384,5280GC10.4% 139.8 / 7.6 48G331A (GGC→GCC) ‡lldPL‑lactate permease
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (2/3);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

TCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCA  >  NC_002947/5384381‑5384665
                                                                                                                                                   |                                                                                                                                         
tCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggc                                                                                                                                          >  6:7753/1‑149 (MQ=255)
tCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggc                                                                                                                                          >  1:239044/1‑149 (MQ=255)
  ggCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggcgg                                                                                                                                        <  6:148096/149‑1 (MQ=255)
   gcgcAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGATCGCCGTTCCTGATCCTCAACGTGCTGGTCACCATCTGGACAATGAAGCCGTTCAAGGCGGCTTTCGCGCCCggcggc                                                                                                                                       >  7:40281/1‑149 (MQ=255)
        gCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGAt                                                                                                                                  <  1:74255/149‑1 (MQ=255)
         ccGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGaaa                                                                                                                                 <  3:64502/149‑3 (MQ=255)
           gTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGaaagc                                                                                                                               <  8:103469/149‑5 (MQ=255)
            ttGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTAc                                                                                                                              <  5:7753/149‑1 (MQ=255)
                  aGCCAAGCGTCAGCCAGCCTATCCCGTACAGCTTTGGCGATATCTTCATGGCCTGGTCGCCGTTCCTGATCCTCACTGTGCTTGTCACCATCTTGACTCTGAAGTCGTTCAAGGTGGCTTTTGCGCCGGGCGGCGCGATGTACAACTTc                                                                                                                        <  8:40281/149‑1 (MQ=255)
                     cAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg                                                                                                                     >  5:47796/1‑149 (MQ=255)
                      aaGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtgt                                                                                                                    <  2:226493/149‑1 (MQ=255)
                      aaGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                      >  7:137771/1‑147 (MQ=255)
                      aaGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                      <  8:137771/147‑1 (MQ=255)
                             agccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggcggc                                                                                                                                       >  4:256714/1‑123 (MQ=255)
                             agccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggcggc                                                                                                                                       <  3:256714/123‑1 (MQ=255)
                             agccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACtt                                                                                                             <  2:236432/149‑1 (MQ=255)
                               ccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                      <  7:100584/138‑1 (MQ=255)
                               ccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgt                                                                                                                      >  8:100584/1‑138 (MQ=255)
                               ccagccTAGCCCGTACAGCTTTGGCGAGATCTACAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCg                                                                                                           >  4:210096/1‑149 (MQ=255)
                                cagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGc                                                                                                          <  2:239044/149‑1 (MQ=255)
                                   ccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCAt                                                                                                       <  7:71213/149‑1 (MQ=255)
                                        cccGTACAGCTTTGGCGAGATCTGCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCg                                                                                                   >  1:232203/1‑148 (MQ=255)
                                               aGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGa                                                                                           >  7:73287/1‑149 (MQ=255)
                                                        gagaTCTACAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGt                                                                                  <  3:210096/149‑1 (MQ=255)
                                                                 aaGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAc                                                                         >  7:137944/1‑149 (MQ=255)
                                                                    gCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAcc                                                                        <  7:246049/147‑1 (MQ=255)
                                                                    gCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAcc                                                                        >  8:246049/1‑147 (MQ=255)
                                                                         gTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCg                                                                  >  2:256348/1‑148 (MQ=255)
                                                                         gTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGa                                                                 >  1:124701/1‑149 (MQ=255)
                                                                            gCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCg                                                                                                           >  6:48770/1‑104 (MQ=255)
                                                                            gCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCg                                                                                                           <  5:48770/104‑1 (MQ=255)
                                                                                  ccTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCACCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCcg                                                        >  4:54663/1‑149 (MQ=255)
                                                                                           cACCGTGCTGGTCAGCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCCCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCg                                               >  7:227813/1‑149 (MQ=255)
                                                                                           cACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCg                                               >  7:91080/1‑149 (MQ=255)
                                                                                               gTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCAt                                           >  6:216418/1‑149 (MQ=255)
                                                                                               gTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCAt                                           <  5:216418/149‑1 (MQ=255)
                                                                                                  cTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGc                                         <  8:137944/148‑1 (MQ=255)
                                                                                                  cTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGc                                         <  6:47796/148‑1 (MQ=255)
                                                                                                       cACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcggcgg                                   >  1:233259/1‑149 (MQ=255)
                                                                                                       cACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcgg                                      >  5:11272/1‑146 (MQ=255)
                                                                                                       cACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcgg                                      <  6:11272/146‑1 (MQ=255)
                                                                                                          cATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtgt                                <  8:73287/149‑1 (MQ=255)
                                                                                                                   ccTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCCCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCg                       <  8:227813/149‑1 (MQ=255)
                                                                                                                     tGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAc                     >  5:265183/1‑149 (MQ=255)
                                                                                                                         gCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAccc                   >  4:74787/1‑147 (MQ=255)
                                                                                                                         gCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAccc                   <  3:74787/147‑1 (MQ=255)
                                                                                                                               cAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc            <  8:91080/148‑1 (MQ=255)
                                                                                                                               cAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc            <  2:124701/148‑1 (MQ=255)
                                                                                                                                 aGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTACGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGc          >  1:199417/1‑148 (MQ=255)
                                                                                                                                   gcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCAc       <  1:256348/149‑1 (MQ=255)
                                                                                                                                       cTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCgg    >  4:256177/1‑148 (MQ=255)
                                                                                                                                        catCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtg                                 >  3:195897/3‑118 (MQ=255)
                                                                                                                                        catCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtg                                 <  4:195897/116‑1 (MQ=255)
                                                                                                                                        catCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCa  >  1:116899/3‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
TCGGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCA  >  NC_002947/5384381‑5384665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑