Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,387,364 C→T 8.5% Q292* (CAG→TAG)  dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,387,3640CT 8.5% 129.3 / 3.0 47Q292* (CAG→TAG) dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GCACCTGCTGGTCGGCTCCGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCCTGCTGC  >  NC_002947/5387216‑5387511
                                                                                                                                                    |                                                                                                                                                    
gcaCCTGCTGGTCGGCTCCGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAagcagc                                                                                                                                                      <  5:95435/149‑1 (MQ=255)
    ctgctgGTCGGCTCCGAAGGCACGCTGGGCTTCCTCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCCGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGcc                                                                                                                                                  >  7:193151/1‑149 (MQ=255)
        tgGTCGGCTCCGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGtc                                                                                                                                              >  7:222494/1‑149 (MQ=255)
          gTCGGCTCCGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGtc                                                                                                                                              <  3:74830/147‑1 (MQ=255)
                  cGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTa                                                                                                                                     <  5:95288/148‑1 (MQ=255)
                                  ttCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGAccgccg                                                                                                                    >  5:58393/1‑149 (MQ=255)
                                  ttCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGAccgccg                                                                                                                    >  5:153482/1‑149 (MQ=255)
                                     atcaGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCa                                                                                                                  <  5:229375/148‑1 (MQ=255)
                                     atcaGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAg                                                                                                                 >  2:259599/1‑149 (MQ=255)
                                                 aCGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGTTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCgg                                                                                                     <  3:138884/148‑1 (MQ=255)
                                                  cGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTg                                                                                                   <  8:8247/149‑1 (MQ=255)
                                                       gACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGaa                                                                                              >  1:113963/1‑149 (MQ=255)
                                                       gACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGaa                                                                                              <  7:58219/149‑1 (MQ=255)
                                                       gACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTACCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCACGCAACAGCCGGTCTCGGCCGGAGAACTGCTCGACCGCCGCAG‑CCTCCGCTCGGTGCAGaa                                                                                              >  5:200933/1‑149 (MQ=255)
                                                         cacCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACa                                                                                            >  3:98120/1‑149 (MQ=255)
                                                          acCGTGCCCGACCACCCGCACAAGGGCTGCGCCCTGCTGGTGTTCCCCAGCTTTGAAAGCTGCGGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACAt                                                                                           <  7:246936/149‑1 (MQ=255)
                                                                cccGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCgg                                                                                     >  2:80971/1‑149 (MQ=255)
                                                                        cccGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGc                                                                             >  5:260772/1‑149 (MQ=255)
                                                                              caAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCgg                                                                                     >  4:214092/1‑135 (MQ=255)
                                                                              caAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCgg                                                                                     <  3:214092/135‑1 (MQ=255)
                                                                               aaGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGACCGGCATGCCGCTGTggg                                                                       >  6:6021/1‑148 (MQ=255)
                                                                                      gcgcCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGc                                                               <  4:196659/149‑1 (MQ=255)
                                                                                          ccTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGTAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTga                                                           >  1:230617/1‑148 (MQ=255)
                                                                                          ccTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGTAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGt                                                           >  5:71526/1‑149 (MQ=255)
                                                                                             gctgGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCg                                                         >  5:30216/1‑148 (MQ=255)
                                                                                                   gtTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATg                                                  >  3:248290/1‑149 (MQ=255)
                                                                                                    ttCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGc                                                 <  2:113963/149‑1 (MQ=255)
                                                                                                    ttCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGc                                                 <  6:260772/149‑1 (MQ=255)
                                                                                                      ccccAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAGATCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGc                                                 <  1:80971/148‑1 (MQ=255)
                                                                                                       cccAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGc                                                                                >  4:204095/1‑115 (MQ=255)
                                                                                                       cccAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGc                                                                                <  3:204095/115‑1 (MQ=255)
                                                                                                       cccAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTg                                              >  5:275270/1‑149 (MQ=255)
                                                                                                       cccAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTg                                              <  6:275270/149‑1 (MQ=255)
                                                                                                         cAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTgcg                                            <  6:58393/149‑1 (MQ=255)
                                                                                                              ttGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGTAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctg                                       <  2:230617/149‑1 (MQ=255)
                                                                                                              ttGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGTAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctg                                       <  6:71526/149‑1 (MQ=255)
                                                                                                              ttGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCACGGCATGCCGCTGTGGGTAAAAGGCATGTCGGACAATGCCTGCGCCctg                                       >  2:103416/1‑149 (MQ=255)
                                                                                                              ttGAAAGCGGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctg                                       <  8:193151/149‑1 (MQ=255)
                                                                                                                 aaaGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctgctg                                    <  6:30216/149‑1 (MQ=255)
                                                                                                                 aaaGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctgct                                     <  6:191462/148‑1 (MQ=255)
                                                                                                                       gctgcCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGa                               >  2:46074/1‑148 (MQ=255)
                                                                                                                        ctgcCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGaa                              <  4:248290/148‑1 (MQ=255)
                                                                                                                            cgcgcgGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctg                                       <  3:27645/135‑1 (MQ=255)
                                                                                                                            cgcgcgGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCctg                                       >  4:27645/1‑135 (MQ=255)
                                                                                                                                     cccGTGGTCAAGCATCAGCCGGTCTCGGCCGTAGAACTGCTCGACCTCCGCAG‑CCTGCGCTCGGTGCATATCATGCCGGGCATGCCGCTGTGGGTTAAAGGCCTCTCGGACAATGCCTGCGCCCTGCTGATCGAATCGCGCGCCgcca                 <  6:200933/148‑1 (MQ=255)
                                                                                                                                       cGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCgccagc               >  4:26862/1‑148 (MQ=255)
                                                                                                                                        gTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCgccagcca             >  6:209194/1‑149 (MQ=255)
                                                                                                                                            tCAAGCAGCAGCCGGTCTCGGCCGTAGAACTCCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGc         >  1:18719/1‑149 (MQ=255)
                                                                                                                                               agcagcagcCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCctg      >  4:193139/1‑149 (MQ=255)
                                                                                                                                                   gcagcCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCctgctg   >  5:16164/1‑148 (MQ=255)
                                                                                                                                                   gcagcCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCCtgctgc  <  5:209194/149‑1 (MQ=255)
                                                                                                                                                   gcagcCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCCtgctgc  >  1:199954/1‑149 (MQ=255)
                                                                                                                                                   gcagcCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCCCCAGCCAGAGCctgctg   >  5:20117/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCACCTGCTGGTCGGCTCCGAAGGCACGCTGGGCTTCATCAGCGCCGTCACGTACGACACCGTGCCCGACCACCCGCACAAGGCCAGCGCCCTGCTGGTGTTCCCCAGCGTTGAAAGCTGCTGCCGCGCGGTACCCGTGCTCAAGCAGCAGCCGGTCTCGGCCGTAGAACTGCTCGACCGCCGCAG‑CCTGCGCTCGGTGCAGAACATGCCCGGCATGCCGCTGTGGGTAAAAGGCCTGTCGGACAATGCCTGCGCCCTGCTGATCGAATCCCGCGCCGCCAGCCAGAGCCTGCTGC  >  NC_002947/5387216‑5387511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑