Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,393,124 C→T 6.9% H22H (CAC→CAT) ‡ hsdR → type I restriction modification system endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,393,1240CT 6.9% 170.7 / 2.3 58H22H (CAC→CAT) ‡hsdRtype I restriction modification system endonuclease
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base T (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

TGAGAGCCCCCCGACGATAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGCCAACCTGCCTATCGCTGT  >  NC_002947/5392977‑5393270
                                                                                                                                                    |                                                                                                                                                  
tGAGAGCCCCCCGACGATAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACg                                                                                                                                                   >  2:41273/1‑149 (MQ=255)
         cccGACGATAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCtgggtgg                                                                                                                                           >  7:217715/1‑148 (MQ=255)
          ccGACGATAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGa                                                                                                                                         >  7:46495/1‑149 (MQ=255)
           cgacgaTAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGAc                                                                                                                                        <  8:20823/149‑1 (MQ=255)
              cgaTAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATg                                                                                                                                     <  8:46495/149‑1 (MQ=255)
               gaTAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATACCATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATg                                                                                                                                     <  1:44911/149‑1 (MQ=255)
                  aGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGcaca                                                                                                                                 <  5:83521/149‑1 (MQ=255)
                     ggCAGCATACCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACaaa                                                                                                                               >  1:98412/1‑148 (MQ=255)
                      gcagcaTGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAAc                                                                                                                              <  5:132338/148‑1 (MQ=255)
                       cagcaTGCCTCTAGCTATTTTTTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAg                                                                                                                            <  8:82277/149‑1 (MQ=255)
                          caTGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCtgggtgg                                                                                                                                           <  4:194076/131‑1 (MQ=255)
                          caTGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCtgggtgg                                                                                                                                           >  3:194076/1‑131 (MQ=255)
                           aTGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGc                                                                                                                        <  1:111217/149‑1 (MQ=255)
                           aTGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGc                                                                                                                        <  2:221834/149‑1 (MQ=255)
                              ccTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGt                                                                                                                      >  3:212891/1‑148 (MQ=255)
                                tctAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTg                                                                                                                     >  7:137293/1‑147 (MQ=255)
                                  tAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGaa                                                                                                                   <  8:217715/147‑1 (MQ=255)
                                      tATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGt                                                                                                              >  3:164604/1‑148 (MQ=255)
                                        ttttGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTc                                                                                                             <  1:41273/147‑1 (MQ=255)
                                        ttttGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCa                                                                                                            <  8:137293/148‑1 (MQ=255)
                                            gTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCtt                                                                                                        <  3:114043/148‑1 (MQ=255)
                                                  ttCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCaaa                                                                                                 <  6:127639/149‑1 (MQ=255)
                                                      gCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGcc                                                                                             >  8:207250/1‑149 (MQ=255)
                                                           acGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGc                                                                                                                        <  5:51302/117‑1 (MQ=255)
                                                           acGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCAGCCAGCACGCTGGGTGGGACATGCACAAACAGGTGc                                                                                                                        >  6:51302/1‑117 (MQ=255)
                                                                    ggACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGt                                                                               >  5:95437/1‑149 (MQ=255)
                                                                         tAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGc                                                                            >  7:27969/1‑147 (MQ=255)
                                                                             cAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATg                                                                                     >  8:94106/1‑134 (MQ=255)
                                                                             cAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATg                                                                                     <  7:94106/134‑1 (MQ=255)
                                                                             cAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCt                                                                       >  3:19142/1‑148 (MQ=255)
                                                                              aGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCaa                                                                                                  >  3:89829/1‑120 (MQ=255)
                                                                              aGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCaa                                                                                                  <  4:89829/120‑1 (MQ=255)
                                                                                       cAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCggg                                                            >  8:52331/1‑149 (MQ=255)
                                                                                        aaGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCgggg                                                           <  7:207250/149‑1 (MQ=255)
                                                                                          gAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGc                                                                                                          >  4:216316/1‑100 (MQ=255)
                                                                                          gAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGc                                                                                                          <  3:216316/100‑1 (MQ=255)
                                                                                           aaaTCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCg                                                         >  3:97995/1‑148 (MQ=255)
                                                                                                  tttCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGc                                                 >  8:4681/1‑149 (MQ=255)
                                                                                                            ggACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACt                                       >  7:190267/1‑149 (MQ=255)
                                                                                                                 tCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATc                                  >  4:183190/1‑149 (MQ=255)
                                                                                                                   tGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCct                                >  7:246779/1‑149 (MQ=255)
                                                                                                                     catcaAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATc                                  <  4:164604/142‑1 (MQ=255)
                                                                                                                       gcaAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTac                            <  4:97995/149‑1 (MQ=255)
                                                                                                                          aaTA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTaccac                         <  6:95437/149‑1 (MQ=255)
                                                                                                                           aTA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTaccac                         >  7:70725/1‑148 (MQ=255)
                                                                                                                               cATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCaa                      <  7:52331/148‑1 (MQ=255)
                                                                                                                               cATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAg                     <  2:98412/149‑1 (MQ=255)
                                                                                                                               cATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAg                     >  2:146374/1‑149 (MQ=255)
                                                                                                                                aTCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGc                    >  4:155470/1‑149 (MQ=255)
                                                                                                                                aTCGCTCCAGCCATCCAGCACGCTGGGTGGGACAAGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCGTCTACCA‑CAAGcc                   >  6:220842/1‑149 (MQ=255)
                                                                                                                                          ccACCCAGCGTGCTGGATGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTa                             <  5:2915/130‑1 (MQ=255)
                                                                                                                                          ccACCCAGCGTGCTGGATGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTa                             >  6:2915/1‑130 (MQ=255)
                                                                                                                                           cacccAGCGTGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGCCAACCTGc            <  6:86644/143‑1 (MQ=255)
                                                                                                                                           cacccAGCGTGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGCCAACCTGc            >  5:86644/4‑146 (MQ=255)
                                                                                                                                           cATCGAGCTCGATGTGTTGGACATGCACAAACATGTTCGTGAATAGGTCATCTTCAACAAAGGCCGTATTATTGTCCGTTTAAAGATTCACAGCCGGGGCGAATCACTCCGTGCAGACTTCATCCTATACCACCAAGCCAACCTGCCt          <  5:50612/148‑1 (MQ=255)
                                                                                                                                               cAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGc                                                          >  4:20322/1‑96 (MQ=255)
                                                                                                                                               cAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCGTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGc                                                          <  3:20322/96‑1 (MQ=255)
                                                                                                                                                  cACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGCCAACCTGCCTATCGCtgt  <  7:4681/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGAGAGCCCCCCGACGATAGTGGCAGCATGCCTCTAGCTATTTTGTTAAATTCGGCAACACGCCATAGGGACATAGCCAGCAATGGACAAGAAATCACTTTCCGAGCGGGACATCTGCAGCAAATA‑CATCGCTCCAGCCATCCAGCACGCTGGGTGGGACATGCACAAACAGGTGCGTGAAGAGGTCAGCTTCACCAAAGGCCGGATTATGGTCCGTGGCAAGCTTCACAGCCGGGGCGAAGCACGCCGTGCAGACTTCATCCTCTACCACCAAGCCAACCTGCCTATCGCTGT  >  NC_002947/5392977‑5393270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑