Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,400,166	C→G	6.6%	L327V (CTT→GTT) ‡	PP_4743 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,400,166	0	C	G	6.6%	183.9 / 3.9	61	L327V (CTT→GTT) ‡	PP_4743	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base G (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

TCTGAAAAACACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCAAAACAACT  >  NC_002947/5400021‑5400314                                                                                                                                                  |                                                                                                                                                           tctGAAAAACACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCttt                                                                                                                                                         >  5:188793/1‑149 (MQ=255)    gAAAAACACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCttt                                                                                                                                                         <  3:241486/146‑1 (MQ=255)    gAAAAACACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCttt                                                                                                                                                         >  4:241486/1‑146 (MQ=255)     aaaaaCACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCtttt                                                                                                                                                        >  6:56493/1‑146 (MQ=255)     aaaaaCACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCtttt                                                                                                                                                        <  5:56493/146‑1 (MQ=255)      aaaaCACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTtctc                                                                                                                                                     <  2:263769/148‑1 (MQ=255)       aaaCACGACAGGCTCCGTATTGTTTACTTAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACATGATTTTTCTGAGAAACCTTTTCTCAc                                                                                                                                                   <  8:172238/149‑1 (MQ=255)         acacGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCGAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGt                                                                                                                                                 <  3:216667/149‑1 (MQ=255)           acGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGACAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAAAGGTTTCTCagaaa                                                                                                                                                >  7:46351/1‑144 (MQ=255)           acGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTg                                                                                                                                                >  5:219192/1‑148 (MQ=255)             gACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGACAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAAAGGTTTCTCagaaaaat                                                                                                                                             <  8:46351/149‑8 (MQ=255)             gACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTg                                                                                                                                                >  1:17216/1‑146 (MQ=255)             gACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAag                                                                                                                                             <  1:34724/149‑1 (MQ=255)                             tttACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACtt                                                                                                                             >  6:276284/1‑149 (MQ=255)                             tttACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACtt                                                                                                                             >  7:80438/1‑149 (MQ=255)                               tACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTc                                                                                                                            <  5:276284/148‑1 (MQ=255)                                     aGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGAt                                                                                                                      <  2:130180/148‑1 (MQ=255)                                      ggCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCg                                                                                                                    <  6:188793/149‑1 (MQ=255)                                          tAACTGATGATATGATTCTGGAACCTGCTTGGAAGAAAATATACCCAGCTCAGAAACGAAGCTTTGCAATCCAGAATGCTTTCGACTGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCGCGATCGAATc                                                                                                                <  8:123050/149‑1 (MQ=255)                                          tAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATc                                                                                                                <  1:135688/149‑1 (MQ=255)                                           aaCTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGaaaaaaa                                                                                                                                >  7:198171/1‑132 (MQ=255)                                             cTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATg                                                                                                             >  6:3404/1‑149 (MQ=255)                                             cTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATg                                                                                                             <  2:5849/149‑1 (MQ=255)                                              tgatgaAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATTCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGt                                                                                                            >  2:254359/1‑149 (MQ=255)                                                        aTTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCttt                                                                                                   >  4:74491/1‑148 (MQ=255)                                                           cTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGa                                                                                               >  6:50527/1‑149 (MQ=255)                                                                aaCTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTtg                                                                                          <  1:170370/149‑1 (MQ=255)                                                                          aCGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AAGCata                                                                           >  2:134440/1‑149 (MQ=255)                                                                            gAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATatg                                                                         <  3:74491/149‑1 (MQ=255)                                                                             aaaaTATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATatg‑a                                                                       <  8:80438/149‑1 (MQ=255)                                                                                  atTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGAtt                                                                  >  7:187355/1‑149 (MQ=255)                                                                                   ttCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTTAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCACCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGAtt                                                                  <  5:33634/148‑1 (MQ=255)                                                                                      ccAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGaa                                                              >  6:51640/1‑149 (MQ=255)                                                                                          cTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGt                                                          <  2:17216/149‑1 (MQ=255)                                                                                           tCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCTTGTTCGCTGTTTTCGATTTTGATG‑‑‑‑‑AGGCTTATG‑ATGATTGGAATGGtc                                                         >  6:203301/1‑149 (MQ=255)                                                                                           tCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGtc                                                         >  5:142445/1‑149 (MQ=255)                                                                                             aGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACATGATTTTTCTGAGAAACCTTTTCTCAGGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGt                                                          <  6:255338/146‑1 (MQ=255)                                                                                             aGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGtct                                                        >  5:158276/1‑148 (MQ=255)                                                                                             aGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTATTCTGAGAAACCTTTTCTCACCTGAAGAGCTTGAAAAAAACTACCCCTAACGAATCATGTTCGATCTATTCGATTTTGATG‑‑‑‑‑AGGAATATG‑ATGATTGGAATGGtct                                                        >  4:264499/1‑148 (MQ=255)                                                                                                          tGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGt                                           <  8:187355/148‑1 (MQ=255)                                                                                                           gCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGGCTCAAAAAAGAAAGTg                                          <  6:219192/148‑1 (MQ=255)                                                                                                                 cAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAg                                    <  5:3404/148‑1 (MQ=255)                                                                                                                             gACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTa                       >  4:170822/1‑149 (MQ=255)                                                                                                                              aCAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTa                       >  2:58665/1‑148 (MQ=255)                                                                                                                                aGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGa                             <  2:111113/140‑1 (MQ=255)                                                                                                                                aGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGa                             >  1:111113/1‑140 (MQ=255)                                                                                                                                aGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTAc                      >  1:201449/1‑147 (MQ=255)                                                                                                                                  gATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAgg                  >  7:241269/1‑149 (MQ=255)                                                                                                                                    tttttCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGt                >  3:34013/1‑149 (MQ=255)                                                                                                                                    tttttCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGt                <  5:51640/149‑1 (MQ=255)                                                                                                                                       ttCTGAGAAAACTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑ATGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGttt              <  5:50527/148‑1 (MQ=255)                                                                                                                                        tCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGAATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTa             >  3:197412/1‑148 (MQ=255)                                                                                                                                         gtGAGAAAAGGTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAgg                  <  6:160306/141‑1 (MQ=255)                                                                                                                                         gtGAGAAAAGGTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAgg                  >  5:160306/2‑142 (MQ=255)                                                                                                                                          tGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATGGTTTAAGATGTATGTATGATTGAAATGGTCTAAAAAAAGAAAGTGACTAAGAAAAAGACCCCTACAAGGGt                >  1:86766/1‑149 (MQ=255)                                                                                                                                           gagaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCa          >  7:169465/1‑148 (MQ=255)                                                                                                                                           gagaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCa          >  6:117053/1‑148 (MQ=255)                                                                                                                                           gagaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCa          >  8:215672/1‑148 (MQ=255)                                                                                                                                            agaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATc           <  1:58665/146‑1 (MQ=255)                                                                                                                                            agaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCaa         <  2:201449/148‑1 (MQ=255)                                                                                                                                             gaAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCaaaa       <  6:142445/149‑1 (MQ=255)                                                                                                                                                aacTTTTCTCACGTGAAGATCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCTATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAATGGTTTATCAAaacaa    <  3:264499/147‑1 (MQ=255)                                                                                                                                                  cTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCAAAACAACt  <  4:34013/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                           TCTGAAAAACACGACAGGCGCCGTATTGTTTACTGAAGGCATAACTGATGAAATGATTCTGGAAACTGCATGGACGAAAATATTCCCAGCTCAGAAACGCAGCTTTGCAATCCAGAATGCTTTCGACAGGATTTTTCTGAGAAACCTTTTCTCACGTGAAGAGCTTGAAAAAAACTTCCCCGATCGAATCATGTTCGCTCTTTTCGATTTTGATG‑‑‑‑‑AGGCATATG‑ATGATTGGAATGGTCTCAAAAAAGAAAGTGACTCAGAAACAGACCCCTACAAGGGTTTATCAAAACAACT  >  NC_002947/5400021‑5400314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑